Enterococcus species diversity in fecal samples of wild marine species by real-time PCR
Analyses using culture-independent molecular techniques have improved our understanding of microbial composition. The aim of this work was to identify and quantify enterococci in fecal samples of wild marine species using real-time quantitative PCR (qPCR). Seven Enterococcus species were examined in...
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ftunivtoronto:oai:localhost:1807/75218 2023-05-15T15:48:28+02:00 Enterococcus species diversity in fecal samples of wild marine species by real-time PCR Weber Medeiros, Aline Blaese Amorim, Derek Tavares, Mauricio de Moura, Tiane Franco, Ana Claudia d’Azevedo, Pedro Alves Frazzon, Jeverson Frazzon, Ana Paula Guedes 2016-09-18 http://hdl.handle.net/1807/75218 http://www.nrcresearchpress.com/doi/abs/10.1139/cjm-2016-0427 unknown NRC Research Press (a division of Canadian Science Publishing) 0008-4166 http://hdl.handle.net/1807/75218 http://www.nrcresearchpress.com/doi/abs/10.1139/cjm-2016-0427 Article 2016 ftunivtoronto 2020-06-17T12:02:26Z Analyses using culture-independent molecular techniques have improved our understanding of microbial composition. The aim of this work was to identify and quantify enterococci in fecal samples of wild marine species using real-time quantitative PCR (qPCR). Seven Enterococcus species were examined in fecal DNA of South American fur seals (Arctocephalus australis), Subantarctic fur seals (Arctocephalus tropicalis), green turtles (Chelonia mydas), Magellanic penguins (Spheniscus magellanicus), snowy-crowned Tern (Sterna trudeaui), white-backed Stilt (Himantopus melanurus), white-chinned Petrels (Procellaria aequinoctialis), red knot (Calidris canutus), black-browed albatross (Thalassarche melanophris). All Enterococcus species evaluated were detected in all fecal samples of wild marine species, with a concentration ranged between 106 to 1012 copies/ng of total DNA. Differences in the enterococci distribution were observed. Enterococcus faecalis and E. mundtii were most abundant in marine mammals. Enterococcus faecalis was frequent in green turtles, Magellanic penguins, snowy-crowned Tern, red knot, black-browed and albatross. Enterococcus hirae and E. gallinarum showed elevated occurrence in white-backed Stilt, and E. faecium in white-chinned Petrel. This study showed highest diversity of enterococci in feces of wild marine species than currently available data, and ensure that culture-independent analysis help us to enhance our understanding about enterococci in gastrointestinal tracts of wild marine species. The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author. Article in Journal/Newspaper Calidris canutus Red Knot University of Toronto: Research Repository T-Space |
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Open Polar |
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University of Toronto: Research Repository T-Space |
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ftunivtoronto |
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unknown |
description |
Analyses using culture-independent molecular techniques have improved our understanding of microbial composition. The aim of this work was to identify and quantify enterococci in fecal samples of wild marine species using real-time quantitative PCR (qPCR). Seven Enterococcus species were examined in fecal DNA of South American fur seals (Arctocephalus australis), Subantarctic fur seals (Arctocephalus tropicalis), green turtles (Chelonia mydas), Magellanic penguins (Spheniscus magellanicus), snowy-crowned Tern (Sterna trudeaui), white-backed Stilt (Himantopus melanurus), white-chinned Petrels (Procellaria aequinoctialis), red knot (Calidris canutus), black-browed albatross (Thalassarche melanophris). All Enterococcus species evaluated were detected in all fecal samples of wild marine species, with a concentration ranged between 106 to 1012 copies/ng of total DNA. Differences in the enterococci distribution were observed. Enterococcus faecalis and E. mundtii were most abundant in marine mammals. Enterococcus faecalis was frequent in green turtles, Magellanic penguins, snowy-crowned Tern, red knot, black-browed and albatross. Enterococcus hirae and E. gallinarum showed elevated occurrence in white-backed Stilt, and E. faecium in white-chinned Petrel. This study showed highest diversity of enterococci in feces of wild marine species than currently available data, and ensure that culture-independent analysis help us to enhance our understanding about enterococci in gastrointestinal tracts of wild marine species. The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author. |
format |
Article in Journal/Newspaper |
author |
Weber Medeiros, Aline Blaese Amorim, Derek Tavares, Mauricio de Moura, Tiane Franco, Ana Claudia d’Azevedo, Pedro Alves Frazzon, Jeverson Frazzon, Ana Paula Guedes |
spellingShingle |
Weber Medeiros, Aline Blaese Amorim, Derek Tavares, Mauricio de Moura, Tiane Franco, Ana Claudia d’Azevedo, Pedro Alves Frazzon, Jeverson Frazzon, Ana Paula Guedes Enterococcus species diversity in fecal samples of wild marine species by real-time PCR |
author_facet |
Weber Medeiros, Aline Blaese Amorim, Derek Tavares, Mauricio de Moura, Tiane Franco, Ana Claudia d’Azevedo, Pedro Alves Frazzon, Jeverson Frazzon, Ana Paula Guedes |
author_sort |
Weber Medeiros, Aline |
title |
Enterococcus species diversity in fecal samples of wild marine species by real-time PCR |
title_short |
Enterococcus species diversity in fecal samples of wild marine species by real-time PCR |
title_full |
Enterococcus species diversity in fecal samples of wild marine species by real-time PCR |
title_fullStr |
Enterococcus species diversity in fecal samples of wild marine species by real-time PCR |
title_full_unstemmed |
Enterococcus species diversity in fecal samples of wild marine species by real-time PCR |
title_sort |
enterococcus species diversity in fecal samples of wild marine species by real-time pcr |
publisher |
NRC Research Press (a division of Canadian Science Publishing) |
publishDate |
2016 |
url |
http://hdl.handle.net/1807/75218 http://www.nrcresearchpress.com/doi/abs/10.1139/cjm-2016-0427 |
genre |
Calidris canutus Red Knot |
genre_facet |
Calidris canutus Red Knot |
op_relation |
0008-4166 http://hdl.handle.net/1807/75218 http://www.nrcresearchpress.com/doi/abs/10.1139/cjm-2016-0427 |
_version_ |
1766383437624639488 |