Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda
Based on the EST sequence from a hemocyte cDNA library, the cathepsin L cDNA of Exopalaemon carinicauda (EcCatL) was cloned by rapid amplification of cDNA ends (RACE). The EcCatL cDNA was 1136 bp in length, which contains an open reading frame (ORF) of 960 bp, encoding a 319 amino-acid polypeptide....
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Kunming Institute of Zoology, Chinese Academy of Sciences
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ftunivtoronto:oai:localhost:1807/64829 2023-05-15T17:54:32+02:00 Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda DUAN, Ya-Fei LIU, Ping LI, Ji-Tao LI, Jian GAO, Bao-Quan CHEN, Ping 2013-03-14 http://hdl.handle.net/1807/64829 http://www.bioline.org.br/abstract?id=zr13007 http://www.bioline.org.br/zr http://www.zoores.ac.cn/ en eng Kunming Institute of Zoology, Chinese Academy of Sciences http://hdl.handle.net/1807/64829 http://www.bioline.org.br/abstract?id=zr13007 http://www.bioline.org.br/zr http://www.zoores.ac.cn/ Copyright 2013 - Kunming Institute of Zoology, the Chinese Academy of Sciences Exopalaemon carinicauda Cathepsin L Gene cloning Gene expression Article 2013 ftunivtoronto 2020-06-17T11:51:49Z Based on the EST sequence from a hemocyte cDNA library, the cathepsin L cDNA of Exopalaemon carinicauda (EcCatL) was cloned by rapid amplification of cDNA ends (RACE). The EcCatL cDNA was 1136 bp in length, which contains an open reading frame (ORF) of 960 bp, encoding a 319 amino-acid polypeptide. Homology analysis revealed that the amino acid sequence of EcCatL was highly conserved with its homologs in other crustaceans. The similarities of EcCatL with the CatL of Palaemonetes varians and Pandalus borealis were 92% and 76%, respectively. Phylogenetic analysis showed that EcCatL was in the same branch as that of Palaemonetes varians. The expression levels of EcCatL in different tissues were analyzed by quantitative real-time PCR. Expression of EcCatL was detected in all tested tissues of E. carinicauda, including hemocytes, gill, hepatopancreas, muscle, ovary, intestine, stomach and eyestalk, with the highest expression level in hepatopancreas. After challenged with Vibrio anguillarum or white spot syndrome virus, the expression of EcCatL were up-regulated in the hemocytes and hepatopancreas of E. carinicauda. Our results implied that EcCatL might play an important role in the prawn immune response. Article in Journal/Newspaper Pandalus borealis University of Toronto: Research Repository T-Space |
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University of Toronto: Research Repository T-Space |
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ftunivtoronto |
language |
English |
topic |
Exopalaemon carinicauda Cathepsin L Gene cloning Gene expression |
spellingShingle |
Exopalaemon carinicauda Cathepsin L Gene cloning Gene expression DUAN, Ya-Fei LIU, Ping LI, Ji-Tao LI, Jian GAO, Bao-Quan CHEN, Ping Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda |
topic_facet |
Exopalaemon carinicauda Cathepsin L Gene cloning Gene expression |
description |
Based on the EST sequence from a hemocyte cDNA library, the cathepsin L cDNA of Exopalaemon carinicauda (EcCatL) was cloned by rapid amplification of cDNA ends (RACE). The EcCatL cDNA was 1136 bp in length, which contains an open reading frame (ORF) of 960 bp, encoding a 319 amino-acid polypeptide. Homology analysis revealed that the amino acid sequence of EcCatL was highly conserved with its homologs in other crustaceans. The similarities of EcCatL with the CatL of Palaemonetes varians and Pandalus borealis were 92% and 76%, respectively. Phylogenetic analysis showed that EcCatL was in the same branch as that of Palaemonetes varians. The expression levels of EcCatL in different tissues were analyzed by quantitative real-time PCR. Expression of EcCatL was detected in all tested tissues of E. carinicauda, including hemocytes, gill, hepatopancreas, muscle, ovary, intestine, stomach and eyestalk, with the highest expression level in hepatopancreas. After challenged with Vibrio anguillarum or white spot syndrome virus, the expression of EcCatL were up-regulated in the hemocytes and hepatopancreas of E. carinicauda. Our results implied that EcCatL might play an important role in the prawn immune response. |
format |
Article in Journal/Newspaper |
author |
DUAN, Ya-Fei LIU, Ping LI, Ji-Tao LI, Jian GAO, Bao-Quan CHEN, Ping |
author_facet |
DUAN, Ya-Fei LIU, Ping LI, Ji-Tao LI, Jian GAO, Bao-Quan CHEN, Ping |
author_sort |
DUAN, Ya-Fei |
title |
Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda |
title_short |
Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda |
title_full |
Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda |
title_fullStr |
Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda |
title_full_unstemmed |
Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda |
title_sort |
cloning and expression analysis of cathepsin l cdna of exopalaemon carinicauda |
publisher |
Kunming Institute of Zoology, Chinese Academy of Sciences |
publishDate |
2013 |
url |
http://hdl.handle.net/1807/64829 http://www.bioline.org.br/abstract?id=zr13007 http://www.bioline.org.br/zr http://www.zoores.ac.cn/ |
genre |
Pandalus borealis |
genre_facet |
Pandalus borealis |
op_relation |
http://hdl.handle.net/1807/64829 http://www.bioline.org.br/abstract?id=zr13007 http://www.bioline.org.br/zr http://www.zoores.ac.cn/ |
op_rights |
Copyright 2013 - Kunming Institute of Zoology, the Chinese Academy of Sciences |
_version_ |
1766162310138691584 |