cDNA cloning and tissue-differential expression of Na+/K+/2Cl−− cotransporter 1-α isoform in Sarotherodon melanotheron
The gills are the major apparatus for osmoregulation in fish to acclimate the changes of salinities. Na+/K+/2Cl− 1-α (NKCC1α) is one of the key ion cotransporter locoalized in gill chloride cells which has been associated with the maintence of osmotic homeostasis. The transport process mediated by N...
Main Authors: | , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | Chinese |
Published: |
Kunming Institute of Zoology, Chinese Academy of Sciences
2011
|
Subjects: | |
Online Access: | http://hdl.handle.net/1807/64603 http://www.bioline.org.br/abstract?id=zr10083 http://www.bioline.org.br/zr http://www.zoores.ac.cn/ |
id |
ftunivtoronto:oai:localhost:1807/64603 |
---|---|
record_format |
openpolar |
spelling |
ftunivtoronto:oai:localhost:1807/64603 2023-05-15T13:28:08+02:00 cDNA cloning and tissue-differential expression of Na+/K+/2Cl−− cotransporter 1-α isoform in Sarotherodon melanotheron Fan, Wu-Jiang Li, Si-fa 2011-06-08 http://hdl.handle.net/1807/64603 http://www.bioline.org.br/abstract?id=zr10083 http://www.bioline.org.br/zr http://www.zoores.ac.cn/ cn chi Kunming Institute of Zoology, Chinese Academy of Sciences http://hdl.handle.net/1807/64603 http://www.bioline.org.br/abstract?id=zr10083 http://www.bioline.org.br/zr http://www.zoores.ac.cn/ Copyright 2010 Kunming Institute of Zoology, the Chinese Academy of Sciences Sarotherodon melanotheron NKCC1α Salt-tolerance Osmoregulation; qRT-PCR Article 2011 ftunivtoronto 2020-06-17T11:51:29Z The gills are the major apparatus for osmoregulation in fish to acclimate the changes of salinities. Na+/K+/2Cl− 1-α (NKCC1α) is one of the key ion cotransporter locoalized in gill chloride cells which has been associated with the maintence of osmotic homeostasis. The transport process mediated by NKCC1α is characterized by electroneutrality with a stoichiometry of 1Na:1K:2Cl. Sarotherodon melanotheron is one of the most euryhaline teleosts able to withstand variations in environmental salinity ranging from freshwater to hyper-saline waters. In this study, the reverse transcription-polymerase chain reaction and rapid amplification of 3' and 5'cDNA ends methods were used to identify the full cDNA of the NKCC1α with an Open Reading Frame which contains 1 151aa of S.melanotheron. The amino acid multiple alignment and phylogenetic analysis showed that this isoform is more similar with isoforms in Oreochromis mossambicus, Salmo salar and Anguilla anguilla, and there is the highest homologous of 99% between Sarotherodon and Mossambique. The predicted protein secondly structure of NKCC1α contains 10 transmenbrane domains, which were highly conserved in sequences and locoalization sites relatively to other species. The quantitative real time polymerase chain reaction (qRT-PCR) assay was developed to estimate the mRNA expression levels in gill, liver, intestine and kidney in freshwater, the results showed a tissue-specific model. Furthermore, the sanility significantly affects the relative expression level of NKCC1α mRNA in gill with a 4.9 times higher in 136 salinity water than that in 0 salinity. The results suggest that the NKCC1α is closely related to the salt tolerance in S.melanotheron. Article in Journal/Newspaper Anguilla anguilla Salmo salar University of Toronto: Research Repository T-Space |
institution |
Open Polar |
collection |
University of Toronto: Research Repository T-Space |
op_collection_id |
ftunivtoronto |
language |
Chinese |
topic |
Sarotherodon melanotheron NKCC1α Salt-tolerance Osmoregulation; qRT-PCR |
spellingShingle |
Sarotherodon melanotheron NKCC1α Salt-tolerance Osmoregulation; qRT-PCR Fan, Wu-Jiang Li, Si-fa cDNA cloning and tissue-differential expression of Na+/K+/2Cl−− cotransporter 1-α isoform in Sarotherodon melanotheron |
topic_facet |
Sarotherodon melanotheron NKCC1α Salt-tolerance Osmoregulation; qRT-PCR |
description |
The gills are the major apparatus for osmoregulation in fish to acclimate the changes of salinities. Na+/K+/2Cl− 1-α (NKCC1α) is one of the key ion cotransporter locoalized in gill chloride cells which has been associated with the maintence of osmotic homeostasis. The transport process mediated by NKCC1α is characterized by electroneutrality with a stoichiometry of 1Na:1K:2Cl. Sarotherodon melanotheron is one of the most euryhaline teleosts able to withstand variations in environmental salinity ranging from freshwater to hyper-saline waters. In this study, the reverse transcription-polymerase chain reaction and rapid amplification of 3' and 5'cDNA ends methods were used to identify the full cDNA of the NKCC1α with an Open Reading Frame which contains 1 151aa of S.melanotheron. The amino acid multiple alignment and phylogenetic analysis showed that this isoform is more similar with isoforms in Oreochromis mossambicus, Salmo salar and Anguilla anguilla, and there is the highest homologous of 99% between Sarotherodon and Mossambique. The predicted protein secondly structure of NKCC1α contains 10 transmenbrane domains, which were highly conserved in sequences and locoalization sites relatively to other species. The quantitative real time polymerase chain reaction (qRT-PCR) assay was developed to estimate the mRNA expression levels in gill, liver, intestine and kidney in freshwater, the results showed a tissue-specific model. Furthermore, the sanility significantly affects the relative expression level of NKCC1α mRNA in gill with a 4.9 times higher in 136 salinity water than that in 0 salinity. The results suggest that the NKCC1α is closely related to the salt tolerance in S.melanotheron. |
format |
Article in Journal/Newspaper |
author |
Fan, Wu-Jiang Li, Si-fa |
author_facet |
Fan, Wu-Jiang Li, Si-fa |
author_sort |
Fan, Wu-Jiang |
title |
cDNA cloning and tissue-differential expression of Na+/K+/2Cl−− cotransporter 1-α isoform in Sarotherodon melanotheron |
title_short |
cDNA cloning and tissue-differential expression of Na+/K+/2Cl−− cotransporter 1-α isoform in Sarotherodon melanotheron |
title_full |
cDNA cloning and tissue-differential expression of Na+/K+/2Cl−− cotransporter 1-α isoform in Sarotherodon melanotheron |
title_fullStr |
cDNA cloning and tissue-differential expression of Na+/K+/2Cl−− cotransporter 1-α isoform in Sarotherodon melanotheron |
title_full_unstemmed |
cDNA cloning and tissue-differential expression of Na+/K+/2Cl−− cotransporter 1-α isoform in Sarotherodon melanotheron |
title_sort |
cdna cloning and tissue-differential expression of na+/k+/2cl−− cotransporter 1-α isoform in sarotherodon melanotheron |
publisher |
Kunming Institute of Zoology, Chinese Academy of Sciences |
publishDate |
2011 |
url |
http://hdl.handle.net/1807/64603 http://www.bioline.org.br/abstract?id=zr10083 http://www.bioline.org.br/zr http://www.zoores.ac.cn/ |
genre |
Anguilla anguilla Salmo salar |
genre_facet |
Anguilla anguilla Salmo salar |
op_relation |
http://hdl.handle.net/1807/64603 http://www.bioline.org.br/abstract?id=zr10083 http://www.bioline.org.br/zr http://www.zoores.ac.cn/ |
op_rights |
Copyright 2010 Kunming Institute of Zoology, the Chinese Academy of Sciences |
_version_ |
1766402422853337088 |