Microsatellites within genes and ESTs of the Pacific oyster Crassostrea gigas and their transferability in five other Crassostrea species
We developed 15 novel polymorphic microsatellites for the Pacific oyster Crassostrea gigas by screening genes and expressed sequence tags (ESTs) found in GenBank. The number of alleles per locus ranged from two to 24 with an average of 8.7, and the values of observed heterozygosity (Ho) and expected...
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Universidad Católica de Valparaíso
2009
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ftunivtoronto:oai:localhost:1807/49024 2023-05-15T15:57:47+02:00 Microsatellites within genes and ESTs of the Pacific oyster Crassostrea gigas and their transferability in five other Crassostrea species Li, Q. Liu, S. Kong, L. 2009-07-30 http://hdl.handle.net/1807/49024 http://www.bioline.org.br/abstract?id=ej09033 http://www.bioline.org.br/ej http://www.ejbiotechnology.info en eng Universidad Católica de Valparaíso http://hdl.handle.net/1807/49024 http://www.bioline.org.br/abstract?id=ej09033 http://www.bioline.org.br/ej http://www.ejbiotechnology.info Copyright 2009 - Pontificia Universidad Católica de Valparaíso -- Chile surface attachment infections indwelling medical devices Article 2009 ftunivtoronto 2020-06-17T11:33:00Z We developed 15 novel polymorphic microsatellites for the Pacific oyster Crassostrea gigas by screening genes and expressed sequence tags (ESTs) found in GenBank. The number of alleles per locus ranged from two to 24 with an average of 8.7, and the values of observed heterozygosity (Ho) and expected heterozygosity (He) ranged from 0.026 to 0.750 and from 0.120 to 0.947, respectively. No significant pairwise linkage disequilibrium was detected among loci and eight loci conformed to Hardy-Weinberg equilibrium. Transferability of the markers was examined on five other Crassostrea species and all the markers were amplified successfully in at least one species. These new microsatellites should be useful for population genetics, parentage analysis and genome mapping studies of C. gigas and closely related species. The nine markers identified from known genes are expected to be especially valuable for comparative mapping as type I markers. Article in Journal/Newspaper Crassostrea gigas Pacific oyster University of Toronto: Research Repository T-Space Pacific |
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Open Polar |
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University of Toronto: Research Repository T-Space |
op_collection_id |
ftunivtoronto |
language |
English |
topic |
surface attachment infections indwelling medical devices |
spellingShingle |
surface attachment infections indwelling medical devices Li, Q. Liu, S. Kong, L. Microsatellites within genes and ESTs of the Pacific oyster Crassostrea gigas and their transferability in five other Crassostrea species |
topic_facet |
surface attachment infections indwelling medical devices |
description |
We developed 15 novel polymorphic microsatellites for the Pacific oyster Crassostrea gigas by screening genes and expressed sequence tags (ESTs) found in GenBank. The number of alleles per locus ranged from two to 24 with an average of 8.7, and the values of observed heterozygosity (Ho) and expected heterozygosity (He) ranged from 0.026 to 0.750 and from 0.120 to 0.947, respectively. No significant pairwise linkage disequilibrium was detected among loci and eight loci conformed to Hardy-Weinberg equilibrium. Transferability of the markers was examined on five other Crassostrea species and all the markers were amplified successfully in at least one species. These new microsatellites should be useful for population genetics, parentage analysis and genome mapping studies of C. gigas and closely related species. The nine markers identified from known genes are expected to be especially valuable for comparative mapping as type I markers. |
format |
Article in Journal/Newspaper |
author |
Li, Q. Liu, S. Kong, L. |
author_facet |
Li, Q. Liu, S. Kong, L. |
author_sort |
Li, Q. |
title |
Microsatellites within genes and ESTs of the Pacific oyster Crassostrea gigas and their transferability in five other Crassostrea species |
title_short |
Microsatellites within genes and ESTs of the Pacific oyster Crassostrea gigas and their transferability in five other Crassostrea species |
title_full |
Microsatellites within genes and ESTs of the Pacific oyster Crassostrea gigas and their transferability in five other Crassostrea species |
title_fullStr |
Microsatellites within genes and ESTs of the Pacific oyster Crassostrea gigas and their transferability in five other Crassostrea species |
title_full_unstemmed |
Microsatellites within genes and ESTs of the Pacific oyster Crassostrea gigas and their transferability in five other Crassostrea species |
title_sort |
microsatellites within genes and ests of the pacific oyster crassostrea gigas and their transferability in five other crassostrea species |
publisher |
Universidad Católica de Valparaíso |
publishDate |
2009 |
url |
http://hdl.handle.net/1807/49024 http://www.bioline.org.br/abstract?id=ej09033 http://www.bioline.org.br/ej http://www.ejbiotechnology.info |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
http://hdl.handle.net/1807/49024 http://www.bioline.org.br/abstract?id=ej09033 http://www.bioline.org.br/ej http://www.ejbiotechnology.info |
op_rights |
Copyright 2009 - Pontificia Universidad Católica de Valparaíso -- Chile |
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1766393484808290304 |