Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings
At present, reforestation has focused on native forests with anthropogenic intervention and eroded soils. There is interest in producing Nothofagus seedlings which can overcome adverse conditions encountered on reforestation sites. It is necessary to find new fungi that can be utilized as mycorrhiza...
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Universidad Católica de Valparaíso
2008
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ftunivtoronto:oai:localhost:1807/48961 2023-05-15T13:32:59+02:00 Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings Valenzuela, E. Hipp, J. Alonso, C. Godoy, R. Alberdi, M. Alvarez, M. Saavedra, I. 2008-12-16 http://hdl.handle.net/1807/48961 http://www.bioline.org.br/abstract?id=ej08026 http://www.bioline.org.br/ej http://www.ejbiotechnology.info en eng Universidad Católica de Valparaíso http://hdl.handle.net/1807/48961 http://www.bioline.org.br/abstract?id=ej08026 http://www.bioline.org.br/ej http://www.ejbiotechnology.info Copyright 2008 - Pontificia Universidad Católica de Valparaíso -- Chile temperature phosphatase pH Nothofagus ectomycorrhizal fungi cellulose Article 2008 ftunivtoronto 2020-06-17T11:32:53Z At present, reforestation has focused on native forests with anthropogenic intervention and eroded soils. There is interest in producing Nothofagus seedlings which can overcome adverse conditions encountered on reforestation sites. It is necessary to find new fungi that can be utilized as mycorrhizal inoculants and that enable the seedlings to increase their tolerance to adverse conditions. Two ectomycorrhizal strains of the fungus Descolea antarctica (D1 and D2) were cultured at different temperatures, pH levels and the activities of amylases, cellulases, and phosphatases were determined. In greenhouse and nursery trials, the growth responses of inoculated Nothofagus obliqua seedlings were evaluated. D1 and D2 exhibited the highest growth rates at 23ºC. Both strains grew at pH levels from 4 to 11. The highest enzymatic activities were registered for amylase (57.2 mg glucose/ml * g of mycelium * hr) and acid phosphatases (58.1 mg p-nitrophenol/ml * g of mycelium * hr) at 37ºC, and acid phospatases (1.720 mg p-nitrophenol/ml * g of mycelium * hr) and alkaline phosphatases (1.360 mg p-nitrophenol/ml * g of mycelium * hr) at pH 4 and pH 11, respectively. We conclude that suitable N. obliqua seedlings for use in reforestation were obtained using D2 as inoculant. Article in Journal/Newspaper Antarc* Antarctica University of Toronto: Research Repository T-Space |
institution |
Open Polar |
collection |
University of Toronto: Research Repository T-Space |
op_collection_id |
ftunivtoronto |
language |
English |
topic |
temperature phosphatase pH Nothofagus ectomycorrhizal fungi cellulose |
spellingShingle |
temperature phosphatase pH Nothofagus ectomycorrhizal fungi cellulose Valenzuela, E. Hipp, J. Alonso, C. Godoy, R. Alberdi, M. Alvarez, M. Saavedra, I. Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings |
topic_facet |
temperature phosphatase pH Nothofagus ectomycorrhizal fungi cellulose |
description |
At present, reforestation has focused on native forests with anthropogenic intervention and eroded soils. There is interest in producing Nothofagus seedlings which can overcome adverse conditions encountered on reforestation sites. It is necessary to find new fungi that can be utilized as mycorrhizal inoculants and that enable the seedlings to increase their tolerance to adverse conditions. Two ectomycorrhizal strains of the fungus Descolea antarctica (D1 and D2) were cultured at different temperatures, pH levels and the activities of amylases, cellulases, and phosphatases were determined. In greenhouse and nursery trials, the growth responses of inoculated Nothofagus obliqua seedlings were evaluated. D1 and D2 exhibited the highest growth rates at 23ºC. Both strains grew at pH levels from 4 to 11. The highest enzymatic activities were registered for amylase (57.2 mg glucose/ml * g of mycelium * hr) and acid phosphatases (58.1 mg p-nitrophenol/ml * g of mycelium * hr) at 37ºC, and acid phospatases (1.720 mg p-nitrophenol/ml * g of mycelium * hr) and alkaline phosphatases (1.360 mg p-nitrophenol/ml * g of mycelium * hr) at pH 4 and pH 11, respectively. We conclude that suitable N. obliqua seedlings for use in reforestation were obtained using D2 as inoculant. |
format |
Article in Journal/Newspaper |
author |
Valenzuela, E. Hipp, J. Alonso, C. Godoy, R. Alberdi, M. Alvarez, M. Saavedra, I. |
author_facet |
Valenzuela, E. Hipp, J. Alonso, C. Godoy, R. Alberdi, M. Alvarez, M. Saavedra, I. |
author_sort |
Valenzuela, E. |
title |
Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings |
title_short |
Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings |
title_full |
Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings |
title_fullStr |
Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings |
title_full_unstemmed |
Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings |
title_sort |
physiological-enzymatic characteristics and inoculation of mycelial strains of descolea antarctica sing. in nothofagus seedlings |
publisher |
Universidad Católica de Valparaíso |
publishDate |
2008 |
url |
http://hdl.handle.net/1807/48961 http://www.bioline.org.br/abstract?id=ej08026 http://www.bioline.org.br/ej http://www.ejbiotechnology.info |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://hdl.handle.net/1807/48961 http://www.bioline.org.br/abstract?id=ej08026 http://www.bioline.org.br/ej http://www.ejbiotechnology.info |
op_rights |
Copyright 2008 - Pontificia Universidad Católica de Valparaíso -- Chile |
_version_ |
1766037438129504256 |