Characterization of widespread canine leishmaniasis among wild carnivores from Spain
Visceral Leishmaniasis (VL) is an emerging zoonotic parasitic disease caused by Leishmania infantum in Mediterranean countries, with sand flies (Phlebotomus spp.) as vectors and dogs as the main domestic reservoir. The role of wild carnivores in the epidemiology of leishmaniasis is still controversi...
Published in: | Veterinary Parasitology |
---|---|
Main Authors: | , , , , , , , , |
Other Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2008
|
Subjects: | |
Online Access: | http://hdl.handle.net/2318/50457 https://doi.org/10.1016/j.vetpar.2008.05.003 |
id |
ftunivtorino:oai:iris.unito.it:2318/50457 |
---|---|
record_format |
openpolar |
spelling |
ftunivtorino:oai:iris.unito.it:2318/50457 2023-11-05T03:41:09+01:00 Characterization of widespread canine leishmaniasis among wild carnivores from Spain R. Sobrino A. Oleaga J. Millan A. Revilla M. C. Arnal C. Gortazar FERROGLIO, Ezio ROMANO, Angelo TRISCIUOGLIO, Anna R. Sobrino E. Ferroglio A. Oleaga A. Romano J. Millan A. Revilla M. C. Arnal A. Trisciuoglio C. Gortazar 2008 http://hdl.handle.net/2318/50457 https://doi.org/10.1016/j.vetpar.2008.05.003 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000258361400004 volume:155 (3-4) firstpage:198 lastpage:203 numberofpages:6 journal:VETERINARY PARASITOLOGY http://hdl.handle.net/2318/50457 doi:10.1016/j.vetpar.2008.05.003 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-47249143210 info:eu-repo/semantics/closedAccess info:eu-repo/semantics/article 2008 ftunivtorino https://doi.org/10.1016/j.vetpar.2008.05.003 2023-10-10T22:17:09Z Visceral Leishmaniasis (VL) is an emerging zoonotic parasitic disease caused by Leishmania infantum in Mediterranean countries, with sand flies (Phlebotomus spp.) as vectors and dogs as the main domestic reservoir. The role of wild carnivores in the epidemiology of leishmaniasis is still controversial. In order to determine the prevalence of natural infection with L. infantum in wild carnivores from Spain, we analyzed 217 samples by PCR and western blotting and used restriction fragment length polymorphism (RFLP) to compare the patterns present in wild carnivores with those of domestic dogs from the same areas. DNA of the parasite was detected in spleen or blood samples from 35 (16.12%) analyzed wild carnivores, including 8 of 39 (20.5%) wolves (Canis lupus), 23 of 162 (14.1%) foxes (Vulpes vulpes), 2 of 7 (28.6%) Egyptian mongooses (Herpestes ichneumon), 1 of 4 genets (Geneta geneta), and 1 of 4 Iberian lynxes (Lynx pardinus). No significant sex or age differences in prevalence were observed in wolves and foxes (P > 0.05), but there was a significant difference among regions in foxes (P < 0.05). A total of 12 PCR-RFLP patterns were found in foxes, 6 in wolves, 4 in dogs, 2 in Egyptian mongooses and 1 in lynx and genet. RFLP patterns differed between dogs and foxes in the two areas where they could be compared. This is the first study of canine leishmaniasis in wild canids and other carnivores from different regions of Spain by PCR. The prevalence of infection indicates the existence of natural infection in apparently healthy wild carnivore populations, and our results are suggestive of a sylvatic cycle independent of dogs. (C) 2008 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Canis lupus Lynx Università degli studi di Torino: AperTo (Archivio Istituzionale ad Accesso Aperto) Veterinary Parasitology 155 3-4 198 203 |
institution |
Open Polar |
collection |
Università degli studi di Torino: AperTo (Archivio Istituzionale ad Accesso Aperto) |
op_collection_id |
ftunivtorino |
language |
English |
description |
Visceral Leishmaniasis (VL) is an emerging zoonotic parasitic disease caused by Leishmania infantum in Mediterranean countries, with sand flies (Phlebotomus spp.) as vectors and dogs as the main domestic reservoir. The role of wild carnivores in the epidemiology of leishmaniasis is still controversial. In order to determine the prevalence of natural infection with L. infantum in wild carnivores from Spain, we analyzed 217 samples by PCR and western blotting and used restriction fragment length polymorphism (RFLP) to compare the patterns present in wild carnivores with those of domestic dogs from the same areas. DNA of the parasite was detected in spleen or blood samples from 35 (16.12%) analyzed wild carnivores, including 8 of 39 (20.5%) wolves (Canis lupus), 23 of 162 (14.1%) foxes (Vulpes vulpes), 2 of 7 (28.6%) Egyptian mongooses (Herpestes ichneumon), 1 of 4 genets (Geneta geneta), and 1 of 4 Iberian lynxes (Lynx pardinus). No significant sex or age differences in prevalence were observed in wolves and foxes (P > 0.05), but there was a significant difference among regions in foxes (P < 0.05). A total of 12 PCR-RFLP patterns were found in foxes, 6 in wolves, 4 in dogs, 2 in Egyptian mongooses and 1 in lynx and genet. RFLP patterns differed between dogs and foxes in the two areas where they could be compared. This is the first study of canine leishmaniasis in wild canids and other carnivores from different regions of Spain by PCR. The prevalence of infection indicates the existence of natural infection in apparently healthy wild carnivore populations, and our results are suggestive of a sylvatic cycle independent of dogs. (C) 2008 Elsevier B.V. All rights reserved. |
author2 |
R. Sobrino E. Ferroglio A. Oleaga A. Romano J. Millan A. Revilla M. C. Arnal A. Trisciuoglio C. Gortazar |
format |
Article in Journal/Newspaper |
author |
R. Sobrino A. Oleaga J. Millan A. Revilla M. C. Arnal C. Gortazar FERROGLIO, Ezio ROMANO, Angelo TRISCIUOGLIO, Anna |
spellingShingle |
R. Sobrino A. Oleaga J. Millan A. Revilla M. C. Arnal C. Gortazar FERROGLIO, Ezio ROMANO, Angelo TRISCIUOGLIO, Anna Characterization of widespread canine leishmaniasis among wild carnivores from Spain |
author_facet |
R. Sobrino A. Oleaga J. Millan A. Revilla M. C. Arnal C. Gortazar FERROGLIO, Ezio ROMANO, Angelo TRISCIUOGLIO, Anna |
author_sort |
R. Sobrino |
title |
Characterization of widespread canine leishmaniasis among wild carnivores from Spain |
title_short |
Characterization of widespread canine leishmaniasis among wild carnivores from Spain |
title_full |
Characterization of widespread canine leishmaniasis among wild carnivores from Spain |
title_fullStr |
Characterization of widespread canine leishmaniasis among wild carnivores from Spain |
title_full_unstemmed |
Characterization of widespread canine leishmaniasis among wild carnivores from Spain |
title_sort |
characterization of widespread canine leishmaniasis among wild carnivores from spain |
publishDate |
2008 |
url |
http://hdl.handle.net/2318/50457 https://doi.org/10.1016/j.vetpar.2008.05.003 |
genre |
Canis lupus Lynx |
genre_facet |
Canis lupus Lynx |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000258361400004 volume:155 (3-4) firstpage:198 lastpage:203 numberofpages:6 journal:VETERINARY PARASITOLOGY http://hdl.handle.net/2318/50457 doi:10.1016/j.vetpar.2008.05.003 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-47249143210 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.vetpar.2008.05.003 |
container_title |
Veterinary Parasitology |
container_volume |
155 |
container_issue |
3-4 |
container_start_page |
198 |
op_container_end_page |
203 |
_version_ |
1781697456749150208 |