Molecular assay for the identification of Setaria tundra
We report the molecular characterization of the amplification products obtained by specific PCR experiments aimed to identify the 5S ribosomal spacer of Setaria tundra specimens isolated from roe deer (Capreolus capreolus) in north Italy, which represent the first record of the parasite in this coun...
Published in: | Veterinary Parasitology |
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Online Access: | http://hdl.handle.net/2318/49597 https://doi.org/10.1016/S0304-4017(03)00210-3 |
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ftunivtorino:oai:iris.unito.it:2318/49597 2023-10-29T02:40:44+01:00 Molecular assay for the identification of Setaria tundra G. Favia G. Cancrini M. Casiraghi I. Ricci FERROGLIO, Ezio ROSSI, Luca G Favia G Cancrini E Ferroglio M Casiraghi I Ricci L Rossi 2003 http://hdl.handle.net/2318/49597 https://doi.org/10.1016/S0304-4017(03)00210-3 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000186831200015 volume:117 (1-2) firstpage:139 lastpage:145 numberofpages:6 journal:VETERINARY PARASITOLOGY http://hdl.handle.net/2318/49597 doi:10.1016/S0304-4017(03)00210-3 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0242298586 info:eu-repo/semantics/article 2003 ftunivtorino https://doi.org/10.1016/S0304-4017(03)00210-3 2023-10-03T22:17:26Z We report the molecular characterization of the amplification products obtained by specific PCR experiments aimed to identify the 5S ribosomal spacer of Setaria tundra specimens isolated from roe deer (Capreolus capreolus) in north Italy, which represent the first record of the parasite in this country. Three different fragments of approximately 400, 800, and 1200 bp (base pairs) are produced. Sequence analyses showed that all three fragments share a very high level of similarity to 5S spacer sequences of some Setaria species and other filariae present in genebank. Based on these sequences, we were able to design species-specific PCR primers for the precise identification of S. tundra. (C) 2003 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Tundra Università degli studi di Torino: AperTo (Archivio Istituzionale ad Accesso Aperto) Veterinary Parasitology 117 1-2 139 145 |
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Open Polar |
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Università degli studi di Torino: AperTo (Archivio Istituzionale ad Accesso Aperto) |
op_collection_id |
ftunivtorino |
language |
English |
description |
We report the molecular characterization of the amplification products obtained by specific PCR experiments aimed to identify the 5S ribosomal spacer of Setaria tundra specimens isolated from roe deer (Capreolus capreolus) in north Italy, which represent the first record of the parasite in this country. Three different fragments of approximately 400, 800, and 1200 bp (base pairs) are produced. Sequence analyses showed that all three fragments share a very high level of similarity to 5S spacer sequences of some Setaria species and other filariae present in genebank. Based on these sequences, we were able to design species-specific PCR primers for the precise identification of S. tundra. (C) 2003 Elsevier B.V. All rights reserved. |
author2 |
G Favia G Cancrini E Ferroglio M Casiraghi I Ricci L Rossi |
format |
Article in Journal/Newspaper |
author |
G. Favia G. Cancrini M. Casiraghi I. Ricci FERROGLIO, Ezio ROSSI, Luca |
spellingShingle |
G. Favia G. Cancrini M. Casiraghi I. Ricci FERROGLIO, Ezio ROSSI, Luca Molecular assay for the identification of Setaria tundra |
author_facet |
G. Favia G. Cancrini M. Casiraghi I. Ricci FERROGLIO, Ezio ROSSI, Luca |
author_sort |
G. Favia |
title |
Molecular assay for the identification of Setaria tundra |
title_short |
Molecular assay for the identification of Setaria tundra |
title_full |
Molecular assay for the identification of Setaria tundra |
title_fullStr |
Molecular assay for the identification of Setaria tundra |
title_full_unstemmed |
Molecular assay for the identification of Setaria tundra |
title_sort |
molecular assay for the identification of setaria tundra |
publishDate |
2003 |
url |
http://hdl.handle.net/2318/49597 https://doi.org/10.1016/S0304-4017(03)00210-3 |
genre |
Tundra |
genre_facet |
Tundra |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000186831200015 volume:117 (1-2) firstpage:139 lastpage:145 numberofpages:6 journal:VETERINARY PARASITOLOGY http://hdl.handle.net/2318/49597 doi:10.1016/S0304-4017(03)00210-3 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0242298586 |
op_doi |
https://doi.org/10.1016/S0304-4017(03)00210-3 |
container_title |
Veterinary Parasitology |
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117 |
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1-2 |
container_start_page |
139 |
op_container_end_page |
145 |
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1781069211175485440 |