Molecular assay for the identification of Setaria tundra

We report the molecular characterization of the amplification products obtained by specific PCR experiments aimed to identify the 5S ribosomal spacer of Setaria tundra specimens isolated from roe deer (Capreolus capreolus) in north Italy, which represent the first record of the parasite in this coun...

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Bibliographic Details
Published in:Veterinary Parasitology
Main Authors: G. Favia, G. Cancrini, M. Casiraghi, I. Ricci, FERROGLIO, Ezio, ROSSI, Luca
Other Authors: G Favia, G Cancrini, E Ferroglio, M Casiraghi, I Ricci, L Rossi
Format: Article in Journal/Newspaper
Language:English
Published: 2003
Subjects:
Tundra
Online Access:http://hdl.handle.net/2318/49597
https://doi.org/10.1016/S0304-4017(03)00210-3
Description
Summary:We report the molecular characterization of the amplification products obtained by specific PCR experiments aimed to identify the 5S ribosomal spacer of Setaria tundra specimens isolated from roe deer (Capreolus capreolus) in north Italy, which represent the first record of the parasite in this country. Three different fragments of approximately 400, 800, and 1200 bp (base pairs) are produced. Sequence analyses showed that all three fragments share a very high level of similarity to 5S spacer sequences of some Setaria species and other filariae present in genebank. Based on these sequences, we were able to design species-specific PCR primers for the precise identification of S. tundra. (C) 2003 Elsevier B.V. All rights reserved.

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