Diversity and small-scale distribution of microbial communities in patterned grounds
Patterned Grounds (PGs) are geometric features formed on permafrost soils as a result of cryoturbation: the mixing, heaving, and churning of soil that occurs during freeze-thaw cycles forms stripes, circles, polygons and nets with and without visible surface textural sorting. Although several studie...
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ftunivtorino:oai:iris.unito.it:2318/1525958 2023-10-25T01:42:49+02:00 Diversity and small-scale distribution of microbial communities in patterned grounds MANIA, ILARIA FREPPAZ, Michele D'AMICO, MICHELE GORRA, ROBERTA Mania, Ilaria Freppaz, Michele D'Amico, Michele Gorra, Roberta 2015 http://hdl.handle.net/2318/1525958 eng eng Conventus country:DEU place:Jena ispartofbook:BAGECO 13 - The microbial continuity across changing ecosystems BAGECO 13 firstpage:119 lastpage:119 numberofpages:1 http://hdl.handle.net/2318/1525958 e info:eu-repo/semantics/openAccess Patterned grounds cryoturbation microbial communities DGGE qPCR info:eu-repo/semantics/conferenceObject 2015 ftunivtorino 2023-09-26T22:26:16Z Patterned Grounds (PGs) are geometric features formed on permafrost soils as a result of cryoturbation: the mixing, heaving, and churning of soil that occurs during freeze-thaw cycles forms stripes, circles, polygons and nets with and without visible surface textural sorting. Although several studies described PGs morphology and their formative processes, microbiological aspects are yet greatly unexplored. Microbial communities that develop in these ecosystems may play important roles in nutrient availability, dynamics and stabilization and therefore in plant colonization and ecosystem evolution. In this study, we focused on circular patterned grounds showing a concentric textural sorting from four areas, characterized by different lithotypes, in the North-western Italian Alps. Samples were subjected to phylogenetic fingerprint by Denaturing Gradient Gel Electrophoresis (DGGE) followed by band sequencing. Moreover, abundance and distribution of bacterial, archaeal and fungal genetic markers and functional genes were measured by quantitative real-time PCR and correlated to chemical parameters. DGGE profiles revealed a relatively homogeneous community structure within circles, but also among different PGs, with several phylotypes previously observed in other PGs and cold environments. Only for Archaea stronger differences in community composition on small-scale were detectable. On a quantitative point of view, microbial populations showed a clear concentric distribution, correlated to C:N ratio, influenced by lithology of parent material and coherent with trends of physicochemical parameters within PG circles. These first results seem to suggest the importance of cryoturbation phenomenon in shaping microbial communities in seasonally frozen soils, at least in a quantitative way, although more detailed functional analysis are needed to make hypothesis about mechanisms involved. Conference Object permafrost Università degli studi di Torino: AperTo (Archivio Istituzionale ad Accesso Aperto) |
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Open Polar |
collection |
Università degli studi di Torino: AperTo (Archivio Istituzionale ad Accesso Aperto) |
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ftunivtorino |
language |
English |
topic |
Patterned grounds cryoturbation microbial communities DGGE qPCR |
spellingShingle |
Patterned grounds cryoturbation microbial communities DGGE qPCR MANIA, ILARIA FREPPAZ, Michele D'AMICO, MICHELE GORRA, ROBERTA Diversity and small-scale distribution of microbial communities in patterned grounds |
topic_facet |
Patterned grounds cryoturbation microbial communities DGGE qPCR |
description |
Patterned Grounds (PGs) are geometric features formed on permafrost soils as a result of cryoturbation: the mixing, heaving, and churning of soil that occurs during freeze-thaw cycles forms stripes, circles, polygons and nets with and without visible surface textural sorting. Although several studies described PGs morphology and their formative processes, microbiological aspects are yet greatly unexplored. Microbial communities that develop in these ecosystems may play important roles in nutrient availability, dynamics and stabilization and therefore in plant colonization and ecosystem evolution. In this study, we focused on circular patterned grounds showing a concentric textural sorting from four areas, characterized by different lithotypes, in the North-western Italian Alps. Samples were subjected to phylogenetic fingerprint by Denaturing Gradient Gel Electrophoresis (DGGE) followed by band sequencing. Moreover, abundance and distribution of bacterial, archaeal and fungal genetic markers and functional genes were measured by quantitative real-time PCR and correlated to chemical parameters. DGGE profiles revealed a relatively homogeneous community structure within circles, but also among different PGs, with several phylotypes previously observed in other PGs and cold environments. Only for Archaea stronger differences in community composition on small-scale were detectable. On a quantitative point of view, microbial populations showed a clear concentric distribution, correlated to C:N ratio, influenced by lithology of parent material and coherent with trends of physicochemical parameters within PG circles. These first results seem to suggest the importance of cryoturbation phenomenon in shaping microbial communities in seasonally frozen soils, at least in a quantitative way, although more detailed functional analysis are needed to make hypothesis about mechanisms involved. |
author2 |
Mania, Ilaria Freppaz, Michele D'Amico, Michele Gorra, Roberta |
format |
Conference Object |
author |
MANIA, ILARIA FREPPAZ, Michele D'AMICO, MICHELE GORRA, ROBERTA |
author_facet |
MANIA, ILARIA FREPPAZ, Michele D'AMICO, MICHELE GORRA, ROBERTA |
author_sort |
MANIA, ILARIA |
title |
Diversity and small-scale distribution of microbial communities in patterned grounds |
title_short |
Diversity and small-scale distribution of microbial communities in patterned grounds |
title_full |
Diversity and small-scale distribution of microbial communities in patterned grounds |
title_fullStr |
Diversity and small-scale distribution of microbial communities in patterned grounds |
title_full_unstemmed |
Diversity and small-scale distribution of microbial communities in patterned grounds |
title_sort |
diversity and small-scale distribution of microbial communities in patterned grounds |
publisher |
Conventus |
publishDate |
2015 |
url |
http://hdl.handle.net/2318/1525958 |
genre |
permafrost |
genre_facet |
permafrost |
op_relation |
ispartofbook:BAGECO 13 - The microbial continuity across changing ecosystems BAGECO 13 firstpage:119 lastpage:119 numberofpages:1 http://hdl.handle.net/2318/1525958 e |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1780739502691581952 |