Nuclear and chloroplast microsatellite markers to assess genetic diversity and evolution in hazelnut species, hybrids and cultivars
We developed new SSR markers and evaluated 114 Corylus accessions representing 11 species and 44 interspecific hybrids. Eight of 23 SSRs generated easyto- score alleles in all species and seven were highly polymorphic. For those seven, the average heterozygosity was moderate at 0.49, and mean allele...
Published in: | Genetic Resources and Crop Evolution |
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ftunivtorino:oai:iris.unito.it:2318/127874 2023-10-29T02:35:21+01:00 Nuclear and chloroplast microsatellite markers to assess genetic diversity and evolution in hazelnut species, hybrids and cultivars Bassil N. Postman J. Mehlenbacher S. BOCCACCI, PAOLO BOTTA, Roberto Bassil N. Boccacci P. Botta R. Postman J. Mehlenbacher S. 2013 STAMPA http://hdl.handle.net/2318/127874 https://doi.org/10.1007/s10722-012-9857-z eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000314361600011 volume:60 firstpage:543 lastpage:568 numberofpages:26 journal:GENETIC RESOURCES AND CROP EVOLUTION http://hdl.handle.net/2318/127874 doi:10.1007/s10722-012-9857-z info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84873522185 info:eu-repo/semantics/openAccess info:eu-repo/semantics/article 2013 ftunivtorino https://doi.org/10.1007/s10722-012-9857-z 2023-10-03T22:24:04Z We developed new SSR markers and evaluated 114 Corylus accessions representing 11 species and 44 interspecific hybrids. Eight of 23 SSRs generated easyto- score alleles in all species and seven were highly polymorphic. For those seven, the average heterozygosity was moderate at 0.49, and mean allele number, genetic diversity and polymorphism information index were high at 11.71, 0.79 and 0.76, respectively. The three most polymorphic SSRs were CaC-C008, CaCC040 and CaC-C118. Neighbor-joining (NJ) clustering and structure analysis agreed with classical taxonomic analysis and supported inclusion of C. maxima within the large polymorphic species, C. avellana. Analysis also indicated that C. californica is a distinct species rather than a botanical variety of C. cornuta. Six universal cpSSRs were polymorphic in Corylus and generated 21 distinct chlorotypes with an average of 3 alleles per locus. Diversity at these cpSSRs was high and ranged from 0.33 to 0.64, with an average of 0.54. Incongruence in NJ topologies between the nuclear and chloroplast markers could be attributed to chloroplast capture related to hybridization during the ancestral diversification of the genus, or to homoplasy. The phylogeographical relationships among the 21 chlorotypes in the 11 Corylus species support Asia as a refugium where several hazelnut lineages survived during glaciation and from which they continued to evolve after dispersal from Asia through the Mediterranean to Europe, and across the Atlantic and/or the Bering land bridge to North America. Article in Journal/Newspaper Bering Land Bridge Università degli studi di Torino: AperTo (Archivio Istituzionale ad Accesso Aperto) Genetic Resources and Crop Evolution 60 2 543 568 |
institution |
Open Polar |
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Università degli studi di Torino: AperTo (Archivio Istituzionale ad Accesso Aperto) |
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ftunivtorino |
language |
English |
description |
We developed new SSR markers and evaluated 114 Corylus accessions representing 11 species and 44 interspecific hybrids. Eight of 23 SSRs generated easyto- score alleles in all species and seven were highly polymorphic. For those seven, the average heterozygosity was moderate at 0.49, and mean allele number, genetic diversity and polymorphism information index were high at 11.71, 0.79 and 0.76, respectively. The three most polymorphic SSRs were CaC-C008, CaCC040 and CaC-C118. Neighbor-joining (NJ) clustering and structure analysis agreed with classical taxonomic analysis and supported inclusion of C. maxima within the large polymorphic species, C. avellana. Analysis also indicated that C. californica is a distinct species rather than a botanical variety of C. cornuta. Six universal cpSSRs were polymorphic in Corylus and generated 21 distinct chlorotypes with an average of 3 alleles per locus. Diversity at these cpSSRs was high and ranged from 0.33 to 0.64, with an average of 0.54. Incongruence in NJ topologies between the nuclear and chloroplast markers could be attributed to chloroplast capture related to hybridization during the ancestral diversification of the genus, or to homoplasy. The phylogeographical relationships among the 21 chlorotypes in the 11 Corylus species support Asia as a refugium where several hazelnut lineages survived during glaciation and from which they continued to evolve after dispersal from Asia through the Mediterranean to Europe, and across the Atlantic and/or the Bering land bridge to North America. |
author2 |
Bassil N. Boccacci P. Botta R. Postman J. Mehlenbacher S. |
format |
Article in Journal/Newspaper |
author |
Bassil N. Postman J. Mehlenbacher S. BOCCACCI, PAOLO BOTTA, Roberto |
spellingShingle |
Bassil N. Postman J. Mehlenbacher S. BOCCACCI, PAOLO BOTTA, Roberto Nuclear and chloroplast microsatellite markers to assess genetic diversity and evolution in hazelnut species, hybrids and cultivars |
author_facet |
Bassil N. Postman J. Mehlenbacher S. BOCCACCI, PAOLO BOTTA, Roberto |
author_sort |
Bassil N. |
title |
Nuclear and chloroplast microsatellite markers to assess genetic diversity and evolution in hazelnut species, hybrids and cultivars |
title_short |
Nuclear and chloroplast microsatellite markers to assess genetic diversity and evolution in hazelnut species, hybrids and cultivars |
title_full |
Nuclear and chloroplast microsatellite markers to assess genetic diversity and evolution in hazelnut species, hybrids and cultivars |
title_fullStr |
Nuclear and chloroplast microsatellite markers to assess genetic diversity and evolution in hazelnut species, hybrids and cultivars |
title_full_unstemmed |
Nuclear and chloroplast microsatellite markers to assess genetic diversity and evolution in hazelnut species, hybrids and cultivars |
title_sort |
nuclear and chloroplast microsatellite markers to assess genetic diversity and evolution in hazelnut species, hybrids and cultivars |
publishDate |
2013 |
url |
http://hdl.handle.net/2318/127874 https://doi.org/10.1007/s10722-012-9857-z |
genre |
Bering Land Bridge |
genre_facet |
Bering Land Bridge |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000314361600011 volume:60 firstpage:543 lastpage:568 numberofpages:26 journal:GENETIC RESOURCES AND CROP EVOLUTION http://hdl.handle.net/2318/127874 doi:10.1007/s10722-012-9857-z info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84873522185 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s10722-012-9857-z |
container_title |
Genetic Resources and Crop Evolution |
container_volume |
60 |
container_issue |
2 |
container_start_page |
543 |
op_container_end_page |
568 |
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1781058473877831680 |