Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities
The study demonstrates the presence of a nodavirus that affected sturgeon in fresh water, causing disease with neurological signs. The virus was isolated and inoculated onto SSN-1 (striped snakehead, Channa striatus) cell cultures where cytopathic effects (CPE) of the virus included vacuolation of t...
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ftunivthessaly:oai:ir.lib.uth.gr:11615/34722 2023-05-15T16:08:39+02:00 Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities Xylouri, E. Kotzamanis, Y. P. Athanassopoulou, F. Dong, L. Pappas, I. S. Argyrokastritis, A. Fragkiadaki, E. 2007 http://hdl.handle.net/11615/34722 unknown 0792-156X http://hdl.handle.net/11615/34722 <Go to ISI>://WOS:000248109000006 Acipenser sp nodavirus isolation characterization sequencing POLYMERASE CHAIN-REACTION NERVOUS-NECROSIS-VIRUS COAT PROTEIN GENE JUVENILE SEA BASS DICENTRARCHUS-LABRAX VIRAL ENCEPHALOPATHY PHYLOGENETIC ANALYSIS NEUROPATHY NODAVIRUS FISH NODAVIRUSES RETINOPATHY Fisheries journalArticle 2007 ftunivthessaly 2021-07-02T06:19:58Z The study demonstrates the presence of a nodavirus that affected sturgeon in fresh water, causing disease with neurological signs. The virus was isolated and inoculated onto SSN-1 (striped snakehead, Channa striatus) cell cultures where cytopathic effects (CPE) of the virus included vacuolation of the cells and degeneration of the monolayer. A 255 bp amplicon from nucleic acid preparations of brain tissue from infected sturgeon was detected by PCR (RT-PCR and nested-PCR) and compared with corresponding amino acid sequences of other infected species. The sequences from the sturgeon were similar to those of sea bass, red spotted grouper, and European eel, supporting the hypothesis that the virus originated in marine fish and was horizontally transmitted to freshwater sturgeon. Article in Journal/Newspaper European eel University of Thessaly Institutional Repository |
institution |
Open Polar |
collection |
University of Thessaly Institutional Repository |
op_collection_id |
ftunivthessaly |
language |
unknown |
topic |
Acipenser sp nodavirus isolation characterization sequencing POLYMERASE CHAIN-REACTION NERVOUS-NECROSIS-VIRUS COAT PROTEIN GENE JUVENILE SEA BASS DICENTRARCHUS-LABRAX VIRAL ENCEPHALOPATHY PHYLOGENETIC ANALYSIS NEUROPATHY NODAVIRUS FISH NODAVIRUSES RETINOPATHY Fisheries |
spellingShingle |
Acipenser sp nodavirus isolation characterization sequencing POLYMERASE CHAIN-REACTION NERVOUS-NECROSIS-VIRUS COAT PROTEIN GENE JUVENILE SEA BASS DICENTRARCHUS-LABRAX VIRAL ENCEPHALOPATHY PHYLOGENETIC ANALYSIS NEUROPATHY NODAVIRUS FISH NODAVIRUSES RETINOPATHY Fisheries Xylouri, E. Kotzamanis, Y. P. Athanassopoulou, F. Dong, L. Pappas, I. S. Argyrokastritis, A. Fragkiadaki, E. Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities |
topic_facet |
Acipenser sp nodavirus isolation characterization sequencing POLYMERASE CHAIN-REACTION NERVOUS-NECROSIS-VIRUS COAT PROTEIN GENE JUVENILE SEA BASS DICENTRARCHUS-LABRAX VIRAL ENCEPHALOPATHY PHYLOGENETIC ANALYSIS NEUROPATHY NODAVIRUS FISH NODAVIRUSES RETINOPATHY Fisheries |
description |
The study demonstrates the presence of a nodavirus that affected sturgeon in fresh water, causing disease with neurological signs. The virus was isolated and inoculated onto SSN-1 (striped snakehead, Channa striatus) cell cultures where cytopathic effects (CPE) of the virus included vacuolation of the cells and degeneration of the monolayer. A 255 bp amplicon from nucleic acid preparations of brain tissue from infected sturgeon was detected by PCR (RT-PCR and nested-PCR) and compared with corresponding amino acid sequences of other infected species. The sequences from the sturgeon were similar to those of sea bass, red spotted grouper, and European eel, supporting the hypothesis that the virus originated in marine fish and was horizontally transmitted to freshwater sturgeon. |
format |
Article in Journal/Newspaper |
author |
Xylouri, E. Kotzamanis, Y. P. Athanassopoulou, F. Dong, L. Pappas, I. S. Argyrokastritis, A. Fragkiadaki, E. |
author_facet |
Xylouri, E. Kotzamanis, Y. P. Athanassopoulou, F. Dong, L. Pappas, I. S. Argyrokastritis, A. Fragkiadaki, E. |
author_sort |
Xylouri, E. |
title |
Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities |
title_short |
Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities |
title_full |
Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities |
title_fullStr |
Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities |
title_full_unstemmed |
Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities |
title_sort |
isolation, characterization, and sequencing of nodavirus in sturgeon (acipenser gueldestaedi l.) reared in freshwater facilities |
publishDate |
2007 |
url |
http://hdl.handle.net/11615/34722 |
genre |
European eel |
genre_facet |
European eel |
op_source |
<Go to ISI>://WOS:000248109000006 |
op_relation |
0792-156X http://hdl.handle.net/11615/34722 |
_version_ |
1766404672784957440 |