Urea Uptake And Carbon Fixation By Marine Pelagic Bacteria And Archaea During The Arctic Summer And Winter Seasons
How Arctic climate change might translate into alterations of biogeochemical cycles of carbon (C) and nitrogen (N) with respect to inorganic and organic N utilization is not well understood. This study combined N-15 uptake rate measurements for ammonium, nitrate, and urea with N-15-and C-13-based DN...
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Online Access: | http://hdl.handle.net/2152/31058 https://doi.org/10.1128/aem.01431-14 |
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ftunivtexas:oai:repositories.lib.utexas.edu:2152/31058 2023-05-15T14:52:03+02:00 Urea Uptake And Carbon Fixation By Marine Pelagic Bacteria And Archaea During The Arctic Summer And Winter Seasons Connelly, Tara L. Baer, Steven E. Cooper, Joshua T. Bronk, Deborah A. Wawrik, Boris Connelly, Tara L. 2014-10 application/pdf http://hdl.handle.net/2152/31058 https://doi.org/10.1128/aem.01431-14 English eng Applied and Environmental Microbiology 0099-2240 http://hdl.handle.net/2152/31058 doi:10.1128/aem.01431-14 Administrative deposit of works to Texas ScholarWorks: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access or the publisher allows a PDF version of the article to be freely posted online. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University. coastal surface waters cape bathurst polynya climate-change heterotrophic bacteria solvent-extraction nutrient dynamics ocean phytoplankton isotope nitrogen biotechnology & applied microbiology microbiology Article 2014 ftunivtexas https://doi.org/10.1128/aem.01431-14 2020-12-23T22:23:38Z How Arctic climate change might translate into alterations of biogeochemical cycles of carbon (C) and nitrogen (N) with respect to inorganic and organic N utilization is not well understood. This study combined N-15 uptake rate measurements for ammonium, nitrate, and urea with N-15-and C-13-based DNA stable-isotope probing (SIP). The objective was to identify active bacterial and archeal plankton and their role in N and C uptake during the Arctic summer and winter seasons. We hypothesized that bacteria and archaea would successfully compete for nitrate and urea during the Arctic winter but not during the summer, when phytoplankton dominate the uptake of these nitrogen sources. Samples were collected at a coastal station near Barrow, AK, during August and January. During both seasons, ammonium uptake rates were greater than those for nitrate or urea, and nitrate uptake rates remained lower than those for ammonium or urea. SIP experiments indicated a strong seasonal shift of bacterial and archaeal N utilization from ammonium during the summer to urea during the winter but did not support a similar seasonal pattern of nitrate utilization. Analysis of 16S rRNA gene sequences obtained from each SIP fraction implicated marine group I Crenarchaeota (MGIC) as well as Betaproteobacteria, Firmicutes, SAR11, and SAR324 in N uptake from urea during the winter. Similarly, C-13 SIP data suggested dark carbon fixation for MGIC, as well as for several proteobacterial lineages and the Firmicutes. These data are consistent with urea-fueled nitrification by polar archaea and bacteria, which may be advantageous under dark conditions. U.S. National Science Foundation OCE 0961900, ARC 0910252, ARC 0909839 Marine Science Article in Journal/Newspaper Arctic Climate change Phytoplankton The University of Texas at Austin: Texas ScholarWorks Arctic Cape Bathurst ENVELOPE(-128.068,-128.068,70.579,70.579) Applied and Environmental Microbiology 80 19 6013 6022 |
institution |
Open Polar |
collection |
The University of Texas at Austin: Texas ScholarWorks |
op_collection_id |
ftunivtexas |
language |
English |
topic |
coastal surface waters cape bathurst polynya climate-change heterotrophic bacteria solvent-extraction nutrient dynamics ocean phytoplankton isotope nitrogen biotechnology & applied microbiology microbiology |
spellingShingle |
coastal surface waters cape bathurst polynya climate-change heterotrophic bacteria solvent-extraction nutrient dynamics ocean phytoplankton isotope nitrogen biotechnology & applied microbiology microbiology Connelly, Tara L. Baer, Steven E. Cooper, Joshua T. Bronk, Deborah A. Wawrik, Boris Urea Uptake And Carbon Fixation By Marine Pelagic Bacteria And Archaea During The Arctic Summer And Winter Seasons |
topic_facet |
coastal surface waters cape bathurst polynya climate-change heterotrophic bacteria solvent-extraction nutrient dynamics ocean phytoplankton isotope nitrogen biotechnology & applied microbiology microbiology |
description |
How Arctic climate change might translate into alterations of biogeochemical cycles of carbon (C) and nitrogen (N) with respect to inorganic and organic N utilization is not well understood. This study combined N-15 uptake rate measurements for ammonium, nitrate, and urea with N-15-and C-13-based DNA stable-isotope probing (SIP). The objective was to identify active bacterial and archeal plankton and their role in N and C uptake during the Arctic summer and winter seasons. We hypothesized that bacteria and archaea would successfully compete for nitrate and urea during the Arctic winter but not during the summer, when phytoplankton dominate the uptake of these nitrogen sources. Samples were collected at a coastal station near Barrow, AK, during August and January. During both seasons, ammonium uptake rates were greater than those for nitrate or urea, and nitrate uptake rates remained lower than those for ammonium or urea. SIP experiments indicated a strong seasonal shift of bacterial and archaeal N utilization from ammonium during the summer to urea during the winter but did not support a similar seasonal pattern of nitrate utilization. Analysis of 16S rRNA gene sequences obtained from each SIP fraction implicated marine group I Crenarchaeota (MGIC) as well as Betaproteobacteria, Firmicutes, SAR11, and SAR324 in N uptake from urea during the winter. Similarly, C-13 SIP data suggested dark carbon fixation for MGIC, as well as for several proteobacterial lineages and the Firmicutes. These data are consistent with urea-fueled nitrification by polar archaea and bacteria, which may be advantageous under dark conditions. U.S. National Science Foundation OCE 0961900, ARC 0910252, ARC 0909839 Marine Science |
author2 |
Connelly, Tara L. |
format |
Article in Journal/Newspaper |
author |
Connelly, Tara L. Baer, Steven E. Cooper, Joshua T. Bronk, Deborah A. Wawrik, Boris |
author_facet |
Connelly, Tara L. Baer, Steven E. Cooper, Joshua T. Bronk, Deborah A. Wawrik, Boris |
author_sort |
Connelly, Tara L. |
title |
Urea Uptake And Carbon Fixation By Marine Pelagic Bacteria And Archaea During The Arctic Summer And Winter Seasons |
title_short |
Urea Uptake And Carbon Fixation By Marine Pelagic Bacteria And Archaea During The Arctic Summer And Winter Seasons |
title_full |
Urea Uptake And Carbon Fixation By Marine Pelagic Bacteria And Archaea During The Arctic Summer And Winter Seasons |
title_fullStr |
Urea Uptake And Carbon Fixation By Marine Pelagic Bacteria And Archaea During The Arctic Summer And Winter Seasons |
title_full_unstemmed |
Urea Uptake And Carbon Fixation By Marine Pelagic Bacteria And Archaea During The Arctic Summer And Winter Seasons |
title_sort |
urea uptake and carbon fixation by marine pelagic bacteria and archaea during the arctic summer and winter seasons |
publishDate |
2014 |
url |
http://hdl.handle.net/2152/31058 https://doi.org/10.1128/aem.01431-14 |
long_lat |
ENVELOPE(-128.068,-128.068,70.579,70.579) |
geographic |
Arctic Cape Bathurst |
geographic_facet |
Arctic Cape Bathurst |
genre |
Arctic Climate change Phytoplankton |
genre_facet |
Arctic Climate change Phytoplankton |
op_relation |
Applied and Environmental Microbiology 0099-2240 http://hdl.handle.net/2152/31058 doi:10.1128/aem.01431-14 |
op_rights |
Administrative deposit of works to Texas ScholarWorks: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access or the publisher allows a PDF version of the article to be freely posted online. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University. |
op_doi |
https://doi.org/10.1128/aem.01431-14 |
container_title |
Applied and Environmental Microbiology |
container_volume |
80 |
container_issue |
19 |
container_start_page |
6013 |
op_container_end_page |
6022 |
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1766323174735085568 |