Anguilla anguilla intestinal immune response to natural infection with Contracaecum rudolphii A larvae
The European eel, Anguilla anguilla, is a major warm-water fish species cultured in North and South Europe. Seventy-one A. anguilla collected between 2010 and 2015 from the Comacchio lagoons were examined. Fish were infected and damaged by larvae (L3) of the nematode Contracaecum rudolphii A, which...
Published in: | Journal of Fish Diseases |
---|---|
Main Authors: | , , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
2016
|
Subjects: | |
Online Access: | http://hdl.handle.net/11575/90500 https://doi.org/10.1111/jfd.12455 |
id |
ftunivteramo:oai:research.unite.it:11575/90500 |
---|---|
record_format |
openpolar |
spelling |
ftunivteramo:oai:research.unite.it:11575/90500 2024-04-14T08:00:59+00:00 Anguilla anguilla intestinal immune response to natural infection with Contracaecum rudolphii A larvae Dezfuli, B. S Bosi, G Depasquale, J. A D'Amelio, S Castaldelli, G Giari, L. MANERA, Maurizio Dezfuli, B. S Manera, Maurizio Bosi, G Depasquale, J. A D'Amelio, S Castaldelli, G Giari, L. 2016 ELETTRONICO http://hdl.handle.net/11575/90500 https://doi.org/10.1111/jfd.12455 unknown info:eu-repo/semantics/altIdentifier/wos/WOS:000387108800005 firstpage:1 lastpage:14 numberofpages:14 journal:JOURNAL OF FISH DISEASES http://hdl.handle.net/11575/90500 doi:10.1111/jfd.12455 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85027957796 European eel fish nematode granuloma immune cell PCNA info:eu-repo/semantics/article 2016 ftunivteramo https://doi.org/10.1111/jfd.12455 2024-03-21T15:31:08Z The European eel, Anguilla anguilla, is a major warm-water fish species cultured in North and South Europe. Seventy-one A. anguilla collected between 2010 and 2015 from the Comacchio lagoons were examined. Fish were infected and damaged by larvae (L3) of the nematode Contracaecum rudolphii A, which were encapsulated within the thickness of the intestinal wall and within the external visceral peritoneum (serosa). Conspicuous granulomas, visible at sites of infection, were arranged in a trilayer, formed by a series of concentric whorls. The cells involved in the immune response and their distribution in the granuloma layers were assessed by immunohistochemical, immunofluorescence, and ultrastructural techniques. The outer part of the granuloma contained macrophages, macrophage aggregates, and mast cells (MCs) scattered among fibroblasts. This layer was vascularized, with degranulation of MCs occurring in close proximity to the capillaries. The middle layer was rich in MCs and fibroblasts. The inner layer, closest to the parasite larva, consisted mainly of dark epithelioid cells, some of which were necrotic. Non-necrotic epithelioid cells formed desmosomes between themselves or with fibroblasts. Within the granulomas, numerous cells of different types were positive to proliferative cell nuclear antigen antibody, indicating a high degree of cellular proliferation around the larvae. Article in Journal/Newspaper Anguilla anguilla European eel IRIS Universita degli Studi di Teramo Journal of Fish Diseases 39 10 1187 1200 |
institution |
Open Polar |
collection |
IRIS Universita degli Studi di Teramo |
op_collection_id |
ftunivteramo |
language |
unknown |
topic |
European eel fish nematode granuloma immune cell PCNA |
spellingShingle |
European eel fish nematode granuloma immune cell PCNA Dezfuli, B. S Bosi, G Depasquale, J. A D'Amelio, S Castaldelli, G Giari, L. MANERA, Maurizio Anguilla anguilla intestinal immune response to natural infection with Contracaecum rudolphii A larvae |
topic_facet |
European eel fish nematode granuloma immune cell PCNA |
description |
The European eel, Anguilla anguilla, is a major warm-water fish species cultured in North and South Europe. Seventy-one A. anguilla collected between 2010 and 2015 from the Comacchio lagoons were examined. Fish were infected and damaged by larvae (L3) of the nematode Contracaecum rudolphii A, which were encapsulated within the thickness of the intestinal wall and within the external visceral peritoneum (serosa). Conspicuous granulomas, visible at sites of infection, were arranged in a trilayer, formed by a series of concentric whorls. The cells involved in the immune response and their distribution in the granuloma layers were assessed by immunohistochemical, immunofluorescence, and ultrastructural techniques. The outer part of the granuloma contained macrophages, macrophage aggregates, and mast cells (MCs) scattered among fibroblasts. This layer was vascularized, with degranulation of MCs occurring in close proximity to the capillaries. The middle layer was rich in MCs and fibroblasts. The inner layer, closest to the parasite larva, consisted mainly of dark epithelioid cells, some of which were necrotic. Non-necrotic epithelioid cells formed desmosomes between themselves or with fibroblasts. Within the granulomas, numerous cells of different types were positive to proliferative cell nuclear antigen antibody, indicating a high degree of cellular proliferation around the larvae. |
author2 |
Dezfuli, B. S Manera, Maurizio Bosi, G Depasquale, J. A D'Amelio, S Castaldelli, G Giari, L. |
format |
Article in Journal/Newspaper |
author |
Dezfuli, B. S Bosi, G Depasquale, J. A D'Amelio, S Castaldelli, G Giari, L. MANERA, Maurizio |
author_facet |
Dezfuli, B. S Bosi, G Depasquale, J. A D'Amelio, S Castaldelli, G Giari, L. MANERA, Maurizio |
author_sort |
Dezfuli, B. S |
title |
Anguilla anguilla intestinal immune response to natural infection with Contracaecum rudolphii A larvae |
title_short |
Anguilla anguilla intestinal immune response to natural infection with Contracaecum rudolphii A larvae |
title_full |
Anguilla anguilla intestinal immune response to natural infection with Contracaecum rudolphii A larvae |
title_fullStr |
Anguilla anguilla intestinal immune response to natural infection with Contracaecum rudolphii A larvae |
title_full_unstemmed |
Anguilla anguilla intestinal immune response to natural infection with Contracaecum rudolphii A larvae |
title_sort |
anguilla anguilla intestinal immune response to natural infection with contracaecum rudolphii a larvae |
publishDate |
2016 |
url |
http://hdl.handle.net/11575/90500 https://doi.org/10.1111/jfd.12455 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000387108800005 firstpage:1 lastpage:14 numberofpages:14 journal:JOURNAL OF FISH DISEASES http://hdl.handle.net/11575/90500 doi:10.1111/jfd.12455 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85027957796 |
op_doi |
https://doi.org/10.1111/jfd.12455 |
container_title |
Journal of Fish Diseases |
container_volume |
39 |
container_issue |
10 |
container_start_page |
1187 |
op_container_end_page |
1200 |
_version_ |
1796305846736519168 |