A non-invasive, improved RIA and overt observation in the study of singleton Apennines wolf (Canis lupus) reproductive behaviour
Abstract. The analysis of fecal hormones allows a close but non-invasive monitoring of animals avoiding the stress of restraint/capture, which in turn can upset animals’ hormonal profile. Steroid hormone progesterone was analysed in three singleton, female grey wolves of different age, belonging to...
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ftunivteramo:oai:research.unite.it:11575/14802 2024-01-28T10:05:03+01:00 A non-invasive, improved RIA and overt observation in the study of singleton Apennines wolf (Canis lupus) reproductive behaviour PROFICO C TRENTINI R GOVONI N BRUGNOLA L STICCO M IANNINO F. M BERNABO', NICOLA MATTIOLI, Mauro LUCIDI, Pia Profico, C Trentini, R Bernabo', Nicola Govoni, N Brugnola, L Sticco, M Iannino, F. M. Mattioli, Mauro Lucidi, Pia 2010 http://hdl.handle.net/11575/14802 http://www.abah.bioflux.com.ro/docs/2010.2.1-9.pdf eng eng volume:2 issue:1 firstpage:1 lastpage:9 numberofpages:9 journal:ANIMAL BIOLOGY & ANIMAL HUSBANDRY http://hdl.handle.net/11575/14802 http://www.abah.bioflux.com.ro/docs/2010.2.1-9.pdf info:eu-repo/semantics/article 2010 ftunivteramo 2024-01-03T17:34:25Z Abstract. The analysis of fecal hormones allows a close but non-invasive monitoring of animals avoiding the stress of restraint/capture, which in turn can upset animals’ hormonal profile. Steroid hormone progesterone was analysed in three singleton, female grey wolves of different age, belonging to the endangered species of the Apennines’ Canis lupus. The analysis was carried out during the breeding season by using an improved radioimmunoassay on samples collected on the field. To reduce the stress to animals and danger to people, the overt observations were carried out by operators who were already familiar with the animals, saving the money of a camera-monitoring-system. Concurrently, a male and a female gray wolves housed together were monitored as a control. The results indicated the importance of dehydration of fecal samples before the extraction with petroleum ether, which was shown to be more efficient than diethyl ether, and that pre-treatment with methanol greatly enhances extraction (p<0.01). Females of Apennines’ grey wolf showed the first sign of oestrus by a vaginal blood loss, that was easily detected on the ground; the analysis carried out on fecal samples revealed a rapidly declining luteal phase, with P4 metabolites reaching the basal values of a non-cyclic female. In the matter of welfare, behavioural observations on Apennines’ grey wolf showed that unpaired animals, although familiar with the operators, failed to display a sexual social behavior during the reproductive season, that is the behavioural signs were hidden in overt observational situation. [.] Article in Journal/Newspaper Canis lupus IRIS Universita degli Studi di Teramo |
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Open Polar |
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IRIS Universita degli Studi di Teramo |
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ftunivteramo |
language |
English |
description |
Abstract. The analysis of fecal hormones allows a close but non-invasive monitoring of animals avoiding the stress of restraint/capture, which in turn can upset animals’ hormonal profile. Steroid hormone progesterone was analysed in three singleton, female grey wolves of different age, belonging to the endangered species of the Apennines’ Canis lupus. The analysis was carried out during the breeding season by using an improved radioimmunoassay on samples collected on the field. To reduce the stress to animals and danger to people, the overt observations were carried out by operators who were already familiar with the animals, saving the money of a camera-monitoring-system. Concurrently, a male and a female gray wolves housed together were monitored as a control. The results indicated the importance of dehydration of fecal samples before the extraction with petroleum ether, which was shown to be more efficient than diethyl ether, and that pre-treatment with methanol greatly enhances extraction (p<0.01). Females of Apennines’ grey wolf showed the first sign of oestrus by a vaginal blood loss, that was easily detected on the ground; the analysis carried out on fecal samples revealed a rapidly declining luteal phase, with P4 metabolites reaching the basal values of a non-cyclic female. In the matter of welfare, behavioural observations on Apennines’ grey wolf showed that unpaired animals, although familiar with the operators, failed to display a sexual social behavior during the reproductive season, that is the behavioural signs were hidden in overt observational situation. [.] |
author2 |
Profico, C Trentini, R Bernabo', Nicola Govoni, N Brugnola, L Sticco, M Iannino, F. M. Mattioli, Mauro Lucidi, Pia |
format |
Article in Journal/Newspaper |
author |
PROFICO C TRENTINI R GOVONI N BRUGNOLA L STICCO M IANNINO F. M BERNABO', NICOLA MATTIOLI, Mauro LUCIDI, Pia |
spellingShingle |
PROFICO C TRENTINI R GOVONI N BRUGNOLA L STICCO M IANNINO F. M BERNABO', NICOLA MATTIOLI, Mauro LUCIDI, Pia A non-invasive, improved RIA and overt observation in the study of singleton Apennines wolf (Canis lupus) reproductive behaviour |
author_facet |
PROFICO C TRENTINI R GOVONI N BRUGNOLA L STICCO M IANNINO F. M BERNABO', NICOLA MATTIOLI, Mauro LUCIDI, Pia |
author_sort |
PROFICO C |
title |
A non-invasive, improved RIA and overt observation in the study of singleton Apennines wolf (Canis lupus) reproductive behaviour |
title_short |
A non-invasive, improved RIA and overt observation in the study of singleton Apennines wolf (Canis lupus) reproductive behaviour |
title_full |
A non-invasive, improved RIA and overt observation in the study of singleton Apennines wolf (Canis lupus) reproductive behaviour |
title_fullStr |
A non-invasive, improved RIA and overt observation in the study of singleton Apennines wolf (Canis lupus) reproductive behaviour |
title_full_unstemmed |
A non-invasive, improved RIA and overt observation in the study of singleton Apennines wolf (Canis lupus) reproductive behaviour |
title_sort |
non-invasive, improved ria and overt observation in the study of singleton apennines wolf (canis lupus) reproductive behaviour |
publishDate |
2010 |
url |
http://hdl.handle.net/11575/14802 http://www.abah.bioflux.com.ro/docs/2010.2.1-9.pdf |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
volume:2 issue:1 firstpage:1 lastpage:9 numberofpages:9 journal:ANIMAL BIOLOGY & ANIMAL HUSBANDRY http://hdl.handle.net/11575/14802 http://www.abah.bioflux.com.ro/docs/2010.2.1-9.pdf |
_version_ |
1789331113518301184 |