Insights into cetacean immunology: Do ecological and biological factors make the difference?
The aim of this study was to evaluate the expression of Major histocompatibility complex (MHC) class I chain-related protein A (MICA) in fibroblast cell cultures of cetaceans (skin biopsies of free-ranging specimens and skin samples of freshly stranded cetaceans) by an immunofluorescence technique a...
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Online Access: | http://hdl.handle.net/11575/103679 https://doi.org/10.3389/fimmu.2019.01219 https://www.frontiersin.org/articles/10.3389/fimmu.2019.00888 |
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ftunivteramo:oai:research.unite.it:11575/103679 2024-04-14T08:09:30+00:00 Insights into cetacean immunology: Do ecological and biological factors make the difference? Marsili L. Di Guardo G. Mazzariol S. Casini S. Yes Marsili, L. Di Guardo, G. Mazzariol, S. Casini, S. 2019 ELETTRONICO http://hdl.handle.net/11575/103679 https://doi.org/10.3389/fimmu.2019.01219 https://www.frontiersin.org/articles/10.3389/fimmu.2019.00888 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000470174600002 numberofpages:11 journal:FRONTIERS IN IMMUNOLOGY http://hdl.handle.net/11575/103679 doi:10.3389/fimmu.2019.01219 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85068484746 https://www.frontiersin.org/articles/10.3389/fimmu.2019.00888 MICA Cetaceans OCs PBDEs PAHs MeHg BPA info:eu-repo/semantics/article 2019 ftunivteramo https://doi.org/10.3389/fimmu.2019.01219 2024-03-21T15:31:49Z The aim of this study was to evaluate the expression of Major histocompatibility complex (MHC) class I chain-related protein A (MICA) in fibroblast cell cultures of cetaceans (skin biopsies of free-ranging specimens and skin samples of freshly stranded cetaceans) by an immunofluorescence technique and to outline possible variations in MICA expression linked to different ecological and biological factors, while also investigating MICA expression after in vitro treatments with different contaminants. Free-ranging or stranded specimens of cetaceans were sampled in the Sea of Cortez (Mexico) (Balaenoptera edeni, Delphinus capensis and Orcinus orca) and in the Mediterranean Sea (Balaenoptera physalus, Physeter macrocephalus, Tursiops truncatus and Stenella coeruleoalba). Cell cultures were treated with an OC mixture, flame retardants, PAHs, MeHg and BPA. The 3 species from the Sea of Cortez showed higher basal activity of MICA and lower levels of DDTs and PCBs than the Mediterranean species. A Pearson's linear coefficient equal to -0.45 also confirmed this tendency to have high levels of MICA and low total OC levels. Treatment of cultured fibroblasts with different contaminants mostly resulted in the upregulation of MICA protein expression by at least one treatment dose; downregulation was also found in some species or treatments. MICA alteration indicates a state of stress of the organism and a modification of the immune system's response and can be proposed as a noninvasive immunological marker that can be measured in skin biopsy samples, thus offering a good alternative to blood measurements. Article in Journal/Newspaper Balaenoptera physalus Orca Orcinus orca Physeter macrocephalus IRIS Universita degli Studi di Teramo Frontiers in Immunology 10 |
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IRIS Universita degli Studi di Teramo |
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topic |
MICA Cetaceans OCs PBDEs PAHs MeHg BPA |
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MICA Cetaceans OCs PBDEs PAHs MeHg BPA Marsili L. Di Guardo G. Mazzariol S. Casini S. Insights into cetacean immunology: Do ecological and biological factors make the difference? |
topic_facet |
MICA Cetaceans OCs PBDEs PAHs MeHg BPA |
description |
The aim of this study was to evaluate the expression of Major histocompatibility complex (MHC) class I chain-related protein A (MICA) in fibroblast cell cultures of cetaceans (skin biopsies of free-ranging specimens and skin samples of freshly stranded cetaceans) by an immunofluorescence technique and to outline possible variations in MICA expression linked to different ecological and biological factors, while also investigating MICA expression after in vitro treatments with different contaminants. Free-ranging or stranded specimens of cetaceans were sampled in the Sea of Cortez (Mexico) (Balaenoptera edeni, Delphinus capensis and Orcinus orca) and in the Mediterranean Sea (Balaenoptera physalus, Physeter macrocephalus, Tursiops truncatus and Stenella coeruleoalba). Cell cultures were treated with an OC mixture, flame retardants, PAHs, MeHg and BPA. The 3 species from the Sea of Cortez showed higher basal activity of MICA and lower levels of DDTs and PCBs than the Mediterranean species. A Pearson's linear coefficient equal to -0.45 also confirmed this tendency to have high levels of MICA and low total OC levels. Treatment of cultured fibroblasts with different contaminants mostly resulted in the upregulation of MICA protein expression by at least one treatment dose; downregulation was also found in some species or treatments. MICA alteration indicates a state of stress of the organism and a modification of the immune system's response and can be proposed as a noninvasive immunological marker that can be measured in skin biopsy samples, thus offering a good alternative to blood measurements. |
author2 |
Yes Marsili, L. Di Guardo, G. Mazzariol, S. Casini, S. |
format |
Article in Journal/Newspaper |
author |
Marsili L. Di Guardo G. Mazzariol S. Casini S. |
author_facet |
Marsili L. Di Guardo G. Mazzariol S. Casini S. |
author_sort |
Marsili L. |
title |
Insights into cetacean immunology: Do ecological and biological factors make the difference? |
title_short |
Insights into cetacean immunology: Do ecological and biological factors make the difference? |
title_full |
Insights into cetacean immunology: Do ecological and biological factors make the difference? |
title_fullStr |
Insights into cetacean immunology: Do ecological and biological factors make the difference? |
title_full_unstemmed |
Insights into cetacean immunology: Do ecological and biological factors make the difference? |
title_sort |
insights into cetacean immunology: do ecological and biological factors make the difference? |
publishDate |
2019 |
url |
http://hdl.handle.net/11575/103679 https://doi.org/10.3389/fimmu.2019.01219 https://www.frontiersin.org/articles/10.3389/fimmu.2019.00888 |
genre |
Balaenoptera physalus Orca Orcinus orca Physeter macrocephalus |
genre_facet |
Balaenoptera physalus Orca Orcinus orca Physeter macrocephalus |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000470174600002 numberofpages:11 journal:FRONTIERS IN IMMUNOLOGY http://hdl.handle.net/11575/103679 doi:10.3389/fimmu.2019.01219 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85068484746 https://www.frontiersin.org/articles/10.3389/fimmu.2019.00888 |
op_doi |
https://doi.org/10.3389/fimmu.2019.01219 |
container_title |
Frontiers in Immunology |
container_volume |
10 |
_version_ |
1796306997587476480 |