Molecular diagnosis of gastroinstestinal viruses in Iberian Wolf (canis lupus signatus) and stray dogs (canis lupus) in northern Portugal
Dissertação de Mestrado Integrado em Medicina Veterinária, área científica de Sanidade Animal Currently, infectious diseases are considered a major threat to the preservation of biodiversity, with viruses emerging as a primary source of outbreaks in wildlife. Consequently, it becomes crucial to moni...
Main Author: | |
---|---|
Other Authors: | , |
Format: | Master Thesis |
Language: | English |
Published: |
Universidade de Lisboa, Faculdade de Medicina Veterinária
2024
|
Subjects: | |
Online Access: | http://hdl.handle.net/10400.5/29705 |
id |
ftunivtecnical:oai:www.repository.utl.pt:10400.5/29705 |
---|---|
record_format |
openpolar |
spelling |
ftunivtecnical:oai:www.repository.utl.pt:10400.5/29705 2024-02-04T09:59:28+01:00 Molecular diagnosis of gastroinstestinal viruses in Iberian Wolf (canis lupus signatus) and stray dogs (canis lupus) in northern Portugal Maia, Inês Sofia da Costa Duarte, Ana Isabel Simões Pereira Boinas, Fernando Jorge Silvano 2024-11-16 http://hdl.handle.net/10400.5/29705 eng eng Universidade de Lisboa, Faculdade de Medicina Veterinária Maia ISC. 2023. Molecular diagnosis of gastroinstestinal viruses in Iberian Wolf (canis lupus signatus) and stray dogs (canis lupus) in northern Portugal [master's thesis]. Lisboa: FMV-Universidade de Lisboa http://hdl.handle.net/10400.5/29705 embargoedAccess http://creativecommons.org/licenses/by/4.0/ Canine Parvovirus Adenovirus Coronavirus Canine Distemper Virus Portugal Parvovírus Canino Esgana Coronavírus Adenovírus Caninos masterThesis 2024 ftunivtecnical 2024-01-10T01:07:32Z Dissertação de Mestrado Integrado em Medicina Veterinária, área científica de Sanidade Animal Currently, infectious diseases are considered a major threat to the preservation of biodiversity, with viruses emerging as a primary source of outbreaks in wildlife. Consequently, it becomes crucial to monitor the presence of viruses within animal populations that exhibit an alarming conservation status, such as the Iberian wolf (Canis lupus signatus). Between 2020 and 2021, 214 stool samples were collected in northern Portugal, of which 199 belonged to Iberian wolves and 15 to stray dogs. With these samples, an initial screening was performed using real-time PCR and conventional PCR for the most common viruses in the Iberian wolf, with the following detection frequencies: canine parvovirus (5.0%), canine distemper virus (5.5%), coronavirus (10.0%), and canine adenovirus (13.5%). In this study, the prevalence rates of canine parvovirus, canine distemper virus, and canine adenovirus were found to be higher in the dog samples compared to the wolves’ samples. This finding underscores the significance of stray dogs in the maintenance of these infectious agents in the habitat. Furthermore, eleven animals from two different packs were identified from 63 samples of Iberian wolves. Based on the collected information, the viral exposure of each individual over time and the presence of these agents in the identified packs, were analyzed. By employing a qPCR genotyping technique, three distinct genotypes of canine parvovirus were identified. Furthermore, the sequencing of the partial Np gene from a CDV positive sample enabled its assignment with the European genotype confirming circulation of non-vaccine strains in the study population. It was not possible to sequence positive samples for canine coronavirus or canine adenovirus in order to carry out the phylogenetic analysis of these samples. However, using the partial sequence of E3 gene of the Adenoviridae family, it was possible to confirm for the first time the presence of ... Master Thesis Canis lupus Technical University of Lisbon: UTL Repository |
institution |
Open Polar |
collection |
Technical University of Lisbon: UTL Repository |
op_collection_id |
ftunivtecnical |
language |
English |
topic |
Canine Parvovirus Adenovirus Coronavirus Canine Distemper Virus Portugal Parvovírus Canino Esgana Coronavírus Adenovírus Caninos |
spellingShingle |
Canine Parvovirus Adenovirus Coronavirus Canine Distemper Virus Portugal Parvovírus Canino Esgana Coronavírus Adenovírus Caninos Maia, Inês Sofia da Costa Molecular diagnosis of gastroinstestinal viruses in Iberian Wolf (canis lupus signatus) and stray dogs (canis lupus) in northern Portugal |
topic_facet |
Canine Parvovirus Adenovirus Coronavirus Canine Distemper Virus Portugal Parvovírus Canino Esgana Coronavírus Adenovírus Caninos |
description |
Dissertação de Mestrado Integrado em Medicina Veterinária, área científica de Sanidade Animal Currently, infectious diseases are considered a major threat to the preservation of biodiversity, with viruses emerging as a primary source of outbreaks in wildlife. Consequently, it becomes crucial to monitor the presence of viruses within animal populations that exhibit an alarming conservation status, such as the Iberian wolf (Canis lupus signatus). Between 2020 and 2021, 214 stool samples were collected in northern Portugal, of which 199 belonged to Iberian wolves and 15 to stray dogs. With these samples, an initial screening was performed using real-time PCR and conventional PCR for the most common viruses in the Iberian wolf, with the following detection frequencies: canine parvovirus (5.0%), canine distemper virus (5.5%), coronavirus (10.0%), and canine adenovirus (13.5%). In this study, the prevalence rates of canine parvovirus, canine distemper virus, and canine adenovirus were found to be higher in the dog samples compared to the wolves’ samples. This finding underscores the significance of stray dogs in the maintenance of these infectious agents in the habitat. Furthermore, eleven animals from two different packs were identified from 63 samples of Iberian wolves. Based on the collected information, the viral exposure of each individual over time and the presence of these agents in the identified packs, were analyzed. By employing a qPCR genotyping technique, three distinct genotypes of canine parvovirus were identified. Furthermore, the sequencing of the partial Np gene from a CDV positive sample enabled its assignment with the European genotype confirming circulation of non-vaccine strains in the study population. It was not possible to sequence positive samples for canine coronavirus or canine adenovirus in order to carry out the phylogenetic analysis of these samples. However, using the partial sequence of E3 gene of the Adenoviridae family, it was possible to confirm for the first time the presence of ... |
author2 |
Duarte, Ana Isabel Simões Pereira Boinas, Fernando Jorge Silvano |
format |
Master Thesis |
author |
Maia, Inês Sofia da Costa |
author_facet |
Maia, Inês Sofia da Costa |
author_sort |
Maia, Inês Sofia da Costa |
title |
Molecular diagnosis of gastroinstestinal viruses in Iberian Wolf (canis lupus signatus) and stray dogs (canis lupus) in northern Portugal |
title_short |
Molecular diagnosis of gastroinstestinal viruses in Iberian Wolf (canis lupus signatus) and stray dogs (canis lupus) in northern Portugal |
title_full |
Molecular diagnosis of gastroinstestinal viruses in Iberian Wolf (canis lupus signatus) and stray dogs (canis lupus) in northern Portugal |
title_fullStr |
Molecular diagnosis of gastroinstestinal viruses in Iberian Wolf (canis lupus signatus) and stray dogs (canis lupus) in northern Portugal |
title_full_unstemmed |
Molecular diagnosis of gastroinstestinal viruses in Iberian Wolf (canis lupus signatus) and stray dogs (canis lupus) in northern Portugal |
title_sort |
molecular diagnosis of gastroinstestinal viruses in iberian wolf (canis lupus signatus) and stray dogs (canis lupus) in northern portugal |
publisher |
Universidade de Lisboa, Faculdade de Medicina Veterinária |
publishDate |
2024 |
url |
http://hdl.handle.net/10400.5/29705 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
Maia ISC. 2023. Molecular diagnosis of gastroinstestinal viruses in Iberian Wolf (canis lupus signatus) and stray dogs (canis lupus) in northern Portugal [master's thesis]. Lisboa: FMV-Universidade de Lisboa http://hdl.handle.net/10400.5/29705 |
op_rights |
embargoedAccess http://creativecommons.org/licenses/by/4.0/ |
_version_ |
1789964286907383808 |