Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.
A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017-2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol,...
Published in: | Microorganisms |
---|---|
Main Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI
2022
|
Subjects: | |
Online Access: | http://hdl.handle.net/11012/204462 https://doi.org/10.3390/microorganisms10040704 |
id |
ftunivtbrno:oai:dspace.vutbr.cz:11012/204462 |
---|---|
record_format |
openpolar |
spelling |
ftunivtbrno:oai:dspace.vutbr.cz:11012/204462 2023-05-15T13:44:03+02:00 Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. Sedláček, Ivo Holochová, Pavla Busse, Hans-Jürgen Koublová, Vendula Králová, Stanislava Švec, Pavel Sobotka, Roman Staňková, Eva Pilný, Jan Šedo, Ondrej Smolíková, Jana Sedlář, Karel 4 10 2022-03-24 text 1-14 application/pdf http://hdl.handle.net/11012/204462 https://doi.org/10.3390/microorganisms10040704 en eng MDPI Microorganisms https://www.mdpi.com/2076-2607/10/4/704/htm 2076-2607 doi:10.3390/microorganisms10040704 http://hdl.handle.net/11012/204462 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ openAccess http://www.sherpa.ac.uk/romeo/issn/2076-2607/ CC-BY Microorganisms. 2022, vol. 10, issue 4, p. 1-14. Massilia violacein psychrophilic description whole-genome sequencing Antarctica article publishedVersion 2022 ftunivtbrno https://doi.org/10.3390/microorganisms10040704 2022-11-22T00:20:47Z A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017-2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, and exhibited strong hydrolytic activities. To clarify the taxonomic position of these isolates, a polyphasic taxonomic approach was applied based on sequencing of the 16S rRNA, gyrB and lepA genes, whole-genome sequencing, rep-PCR, MALDI-TOF MS, chemotaxonomy analyses and biotyping. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the entire group are representatives of the genus Massilia. The closest relatives of the reference strain P8398(T) were Massilia atriviolacea, Massilia violaceinigra, Massilia rubra, Massilia mucilaginosa, Massilia aquatica, Massilia frigida, Massilia glaciei and Massilia eurypsychrophila with a pairwise similarity of 98.6-100% in the 16S rRNA. The subsequent gyrB and lepA sequencing results showed the novelty of the analysed group, and the average nucleotide identity and digital DNA-DNA hybridisation values clearly proved that P8398(T) represents a distinct Massilia species. After all these results, we nominate a new species with the proposed name Massilia antarctica sp. nov. The type strain is P8398(T) (= CCM 8941(T) = LMG 32108(T)). Article in Journal/Newspaper Antarc* Antarctica Eagle Island James Ross Island Ross Island Brno University of Technology (VUT): Digital Library Eagle Island ENVELOPE(-57.489,-57.489,-63.660,-63.660) Ross Island Microorganisms 10 4 704 |
institution |
Open Polar |
collection |
Brno University of Technology (VUT): Digital Library |
op_collection_id |
ftunivtbrno |
language |
English |
topic |
Massilia violacein psychrophilic description whole-genome sequencing Antarctica |
spellingShingle |
Massilia violacein psychrophilic description whole-genome sequencing Antarctica Sedláček, Ivo Holochová, Pavla Busse, Hans-Jürgen Koublová, Vendula Králová, Stanislava Švec, Pavel Sobotka, Roman Staňková, Eva Pilný, Jan Šedo, Ondrej Smolíková, Jana Sedlář, Karel Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
topic_facet |
Massilia violacein psychrophilic description whole-genome sequencing Antarctica |
description |
A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017-2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, and exhibited strong hydrolytic activities. To clarify the taxonomic position of these isolates, a polyphasic taxonomic approach was applied based on sequencing of the 16S rRNA, gyrB and lepA genes, whole-genome sequencing, rep-PCR, MALDI-TOF MS, chemotaxonomy analyses and biotyping. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the entire group are representatives of the genus Massilia. The closest relatives of the reference strain P8398(T) were Massilia atriviolacea, Massilia violaceinigra, Massilia rubra, Massilia mucilaginosa, Massilia aquatica, Massilia frigida, Massilia glaciei and Massilia eurypsychrophila with a pairwise similarity of 98.6-100% in the 16S rRNA. The subsequent gyrB and lepA sequencing results showed the novelty of the analysed group, and the average nucleotide identity and digital DNA-DNA hybridisation values clearly proved that P8398(T) represents a distinct Massilia species. After all these results, we nominate a new species with the proposed name Massilia antarctica sp. nov. The type strain is P8398(T) (= CCM 8941(T) = LMG 32108(T)). |
format |
Article in Journal/Newspaper |
author |
Sedláček, Ivo Holochová, Pavla Busse, Hans-Jürgen Koublová, Vendula Králová, Stanislava Švec, Pavel Sobotka, Roman Staňková, Eva Pilný, Jan Šedo, Ondrej Smolíková, Jana Sedlář, Karel |
author_facet |
Sedláček, Ivo Holochová, Pavla Busse, Hans-Jürgen Koublová, Vendula Králová, Stanislava Švec, Pavel Sobotka, Roman Staňková, Eva Pilný, Jan Šedo, Ondrej Smolíková, Jana Sedlář, Karel |
author_sort |
Sedláček, Ivo |
title |
Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
title_short |
Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
title_full |
Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
title_fullStr |
Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
title_full_unstemmed |
Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
title_sort |
characterisation of waterborne psychrophilic massilia isolates with violacein production and description of massilia antarctica sp. nov. |
publisher |
MDPI |
publishDate |
2022 |
url |
http://hdl.handle.net/11012/204462 https://doi.org/10.3390/microorganisms10040704 |
op_coverage |
4 10 |
long_lat |
ENVELOPE(-57.489,-57.489,-63.660,-63.660) |
geographic |
Eagle Island Ross Island |
geographic_facet |
Eagle Island Ross Island |
genre |
Antarc* Antarctica Eagle Island James Ross Island Ross Island |
genre_facet |
Antarc* Antarctica Eagle Island James Ross Island Ross Island |
op_source |
Microorganisms. 2022, vol. 10, issue 4, p. 1-14. |
op_relation |
Microorganisms https://www.mdpi.com/2076-2607/10/4/704/htm 2076-2607 doi:10.3390/microorganisms10040704 http://hdl.handle.net/11012/204462 |
op_rights |
Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ openAccess http://www.sherpa.ac.uk/romeo/issn/2076-2607/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/microorganisms10040704 |
container_title |
Microorganisms |
container_volume |
10 |
container_issue |
4 |
container_start_page |
704 |
_version_ |
1766196437792587776 |