Flavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov., two multidrug-resistant psychrotrophic species isolated from Antarctica
Despite unfavourable Antarctic conditions, such as cold temperatures, freeze-thaw cycles, high ultraviolet radiation, dryness and lack of nutrients, microorganisms were able to adapt and surprisingly thrive in this environment. In this study, eight cold-adapted Flavobacterium strains isolated from a...
Published in: | Frontiers in Microbiology |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media SA
2021
|
Subjects: | |
Online Access: | http://hdl.handle.net/11012/201796 https://doi.org/10.3389/fmicb.2021.729977 |
id |
ftunivtbrno:oai:dspace.vutbr.cz:11012/201796 |
---|---|
record_format |
openpolar |
spelling |
ftunivtbrno:oai:dspace.vutbr.cz:11012/201796 2023-05-15T14:00:35+02:00 Flavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov., two multidrug-resistant psychrotrophic species isolated from Antarctica Králová, Stanislava Busse, Hans-Jürgen Bezdíček, Matěj Sandoval-Powers, Megan Nykrýnová, Markéta Staňková, Eva Krsek, Daniel Sedláček, Ivo 10 12 2021-10-22 text 1-20 application/pdf http://hdl.handle.net/11012/201796 https://doi.org/10.3389/fmicb.2021.729977 en eng Frontiers Media SA Frontiers in Microbiology https://www.frontiersin.org/articles/10.3389/fmicb.2021.729977/full 1664-302X doi:10.3389/fmicb.2021.729977 http://hdl.handle.net/11012/201796 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ openAccess http://www.sherpa.ac.uk/romeo/issn/1664-302X/ CC-BY Frontiers in Microbiology. 2021, vol. 12, issue 10, p. 1-20. Antarctica psychrophiles cold-adaptation phylogenomics systematics Flavobacterium flabelliforme sp. nov Flavobacterium geliluteum sp. nov article publishedVersion 2021 ftunivtbrno https://doi.org/10.3389/fmicb.2021.729977 2022-02-08T00:18:55Z Despite unfavourable Antarctic conditions, such as cold temperatures, freeze-thaw cycles, high ultraviolet radiation, dryness and lack of nutrients, microorganisms were able to adapt and surprisingly thrive in this environment. In this study, eight cold-adapted Flavobacterium strains isolated from a remote Antarctic island, James Ross Island, were studied using polyphasic taxonomic approach to determine their taxonomic position. Phylogenomic analyses based on 16S rRNA gene and 92 core genes clearly showed that these strains formed two distinct phylogenetic clusters comprising three and five strains, with average nucleotide identities significantly below 90 % in between both proposed species as well as between their closest phylogenetic relatives. Phenotyping revealed a unique pattern of biochemical and physiological characteristics enabling differentiation from the closest phylogenetically related Flavobacterium spp. Chemotaxonomic analyses showed that type strains P4023T and P7388T were characterized by the major polyamine sym-homospermidine and a quinone system containing predominantly menaquinone MK-6. In the polar lipid profile phosphatidylethanolamine, an ornithine lipid and two unidentified lipids lacking a functional group were detected as major lipids. These characteristics along with fatty acid profiles confirmed, that these species belong to the genus Flavobacterium. Thorough genomic analysis revealed presence of numerous cold-inducible or cold-adaptation associated genes, such as cold-shock proteins, proteorhodopsin, carotenoid biosynthetic genes or oxidative-stress response genes. Genomes of type strains surprisingly harboured multiple prophages, with many of them predicted to be active. Genome-mining identified biosynthetic clusters in type strain genomes with majority not matching any known biosynthetic genes which indicates further research possibilities involving these psychrotrophic bacteria. Antibiotic susceptibility testing revealed multidrug-resistant phenotype that was correlated with in silico antibiotic resistance prediction. Interestingly, while typical resistance finder tools failed to detect genes responsible for antibiotic resistance, genomic prediction confirmed multidrug-resistant profile and suggested even broader resistance than tested. Results of this study confirmed and thoroughly characterized two novel psychrotrophic Flavobacterium species, for which names Flavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov. are proposed. Article in Journal/Newspaper Antarc* Antarctic Antarctica James Ross Island Ross Island Brno University of Technology (VUT): Digital Library Antarctic Ross Island Frontiers in Microbiology 12 |
institution |
Open Polar |
collection |
Brno University of Technology (VUT): Digital Library |
op_collection_id |
ftunivtbrno |
language |
English |
topic |
Antarctica psychrophiles cold-adaptation phylogenomics systematics Flavobacterium flabelliforme sp. nov Flavobacterium geliluteum sp. nov |
spellingShingle |
Antarctica psychrophiles cold-adaptation phylogenomics systematics Flavobacterium flabelliforme sp. nov Flavobacterium geliluteum sp. nov Králová, Stanislava Busse, Hans-Jürgen Bezdíček, Matěj Sandoval-Powers, Megan Nykrýnová, Markéta Staňková, Eva Krsek, Daniel Sedláček, Ivo Flavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov., two multidrug-resistant psychrotrophic species isolated from Antarctica |
topic_facet |
Antarctica psychrophiles cold-adaptation phylogenomics systematics Flavobacterium flabelliforme sp. nov Flavobacterium geliluteum sp. nov |
description |
Despite unfavourable Antarctic conditions, such as cold temperatures, freeze-thaw cycles, high ultraviolet radiation, dryness and lack of nutrients, microorganisms were able to adapt and surprisingly thrive in this environment. In this study, eight cold-adapted Flavobacterium strains isolated from a remote Antarctic island, James Ross Island, were studied using polyphasic taxonomic approach to determine their taxonomic position. Phylogenomic analyses based on 16S rRNA gene and 92 core genes clearly showed that these strains formed two distinct phylogenetic clusters comprising three and five strains, with average nucleotide identities significantly below 90 % in between both proposed species as well as between their closest phylogenetic relatives. Phenotyping revealed a unique pattern of biochemical and physiological characteristics enabling differentiation from the closest phylogenetically related Flavobacterium spp. Chemotaxonomic analyses showed that type strains P4023T and P7388T were characterized by the major polyamine sym-homospermidine and a quinone system containing predominantly menaquinone MK-6. In the polar lipid profile phosphatidylethanolamine, an ornithine lipid and two unidentified lipids lacking a functional group were detected as major lipids. These characteristics along with fatty acid profiles confirmed, that these species belong to the genus Flavobacterium. Thorough genomic analysis revealed presence of numerous cold-inducible or cold-adaptation associated genes, such as cold-shock proteins, proteorhodopsin, carotenoid biosynthetic genes or oxidative-stress response genes. Genomes of type strains surprisingly harboured multiple prophages, with many of them predicted to be active. Genome-mining identified biosynthetic clusters in type strain genomes with majority not matching any known biosynthetic genes which indicates further research possibilities involving these psychrotrophic bacteria. Antibiotic susceptibility testing revealed multidrug-resistant phenotype that was correlated with in silico antibiotic resistance prediction. Interestingly, while typical resistance finder tools failed to detect genes responsible for antibiotic resistance, genomic prediction confirmed multidrug-resistant profile and suggested even broader resistance than tested. Results of this study confirmed and thoroughly characterized two novel psychrotrophic Flavobacterium species, for which names Flavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov. are proposed. |
format |
Article in Journal/Newspaper |
author |
Králová, Stanislava Busse, Hans-Jürgen Bezdíček, Matěj Sandoval-Powers, Megan Nykrýnová, Markéta Staňková, Eva Krsek, Daniel Sedláček, Ivo |
author_facet |
Králová, Stanislava Busse, Hans-Jürgen Bezdíček, Matěj Sandoval-Powers, Megan Nykrýnová, Markéta Staňková, Eva Krsek, Daniel Sedláček, Ivo |
author_sort |
Králová, Stanislava |
title |
Flavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov., two multidrug-resistant psychrotrophic species isolated from Antarctica |
title_short |
Flavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov., two multidrug-resistant psychrotrophic species isolated from Antarctica |
title_full |
Flavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov., two multidrug-resistant psychrotrophic species isolated from Antarctica |
title_fullStr |
Flavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov., two multidrug-resistant psychrotrophic species isolated from Antarctica |
title_full_unstemmed |
Flavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov., two multidrug-resistant psychrotrophic species isolated from Antarctica |
title_sort |
flavobacterium flabelliforme sp. nov. and flavobacterium geliluteum sp. nov., two multidrug-resistant psychrotrophic species isolated from antarctica |
publisher |
Frontiers Media SA |
publishDate |
2021 |
url |
http://hdl.handle.net/11012/201796 https://doi.org/10.3389/fmicb.2021.729977 |
op_coverage |
10 12 |
geographic |
Antarctic Ross Island |
geographic_facet |
Antarctic Ross Island |
genre |
Antarc* Antarctic Antarctica James Ross Island Ross Island |
genre_facet |
Antarc* Antarctic Antarctica James Ross Island Ross Island |
op_source |
Frontiers in Microbiology. 2021, vol. 12, issue 10, p. 1-20. |
op_relation |
Frontiers in Microbiology https://www.frontiersin.org/articles/10.3389/fmicb.2021.729977/full 1664-302X doi:10.3389/fmicb.2021.729977 http://hdl.handle.net/11012/201796 |
op_rights |
Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ openAccess http://www.sherpa.ac.uk/romeo/issn/1664-302X/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2021.729977 |
container_title |
Frontiers in Microbiology |
container_volume |
12 |
_version_ |
1766269809143578624 |