Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.
A group of 11 bacterial strains was isolated from streams and lakes located in a deglaciated northern part of James Ross Island, Antarctica. They were rod-shaped, Gram-stain-negative, motile, and catalase-positive and produced blue-violet-pigmented colonies on R2A agar. A polyphasic taxonomic approa...
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American Society for Microbiology
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Online Access: | http://hdl.handle.net/11012/201481 https://doi.org/10.1128/Spectrum.00452-21 |
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ftunivtbrno:oai:dspace.vutbr.cz:11012/201481 2023-05-15T14:00:35+02:00 Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov. Sedláček, Ivo Holochová, Pavla Sobotka, Roman Busse, Hans-Jürgen Švec, Pavel Králová, Stanislava Šedo, Ondrej Pilný, Jan Staňková, Eva Koublová, Vendula Sedlář, Karel 1 9 2021-08-11 text 1-14 application/pdf http://hdl.handle.net/11012/201481 https://doi.org/10.1128/Spectrum.00452-21 en eng American Society for Microbiology Microbiology spectrum https://journals.asm.org/doi/10.1128/Spectrum.00452-21 2165-0497 doi:10.1128/Spectrum.00452-21 http://hdl.handle.net/11012/201481 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ openAccess http://www.sherpa.ac.uk/romeo/issn/2165-0497/ CC-BY Microbiology spectrum. 2021, vol. 9, issue 1, p. 1-14. psychrophiles Rugamonas taxonomy Antarctica violacein species description article publishedVersion 2021 ftunivtbrno https://doi.org/10.1128/Spectrum.00452-21 2022-02-08T00:18:52Z A group of 11 bacterial strains was isolated from streams and lakes located in a deglaciated northern part of James Ross Island, Antarctica. They were rod-shaped, Gram-stain-negative, motile, and catalase-positive and produced blue-violet-pigmented colonies on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, whole-genome sequencing, automated ribotyping, repetitive element sequence-based PCR (rep-PCR), MALDI-TOF MS, fatty acid profile, chemotaxonomy analyses, and extensive biotyping was applied in order to clarify the taxonomic position of these isolates. Phylogenetic analysis based on the 16S rRNA gene indicated that all the isolates constituted a coherent group belonging to the genus Rugamonas. The closest relatives to the representative isolate P5900T were Rugamonas rubra CCM 3730T, Rugamonas rivuli FT103WT, and Rugamonas aquatica FT29WT, exhibiting 99.2%, 99.1%, and 98.6% 16S rRNA pairwise similarity, respectively. The average nucleotide identity and digital DNA-DNA hybridization values calculated from the whole-genome sequencing data clearly proved that P5900T represents a distinct Rugamonas species. The G+C content of genomic DNAs was 66.1mol%. The major components in fatty acid profiles were summed feature 3 (C16:1 7c/C16:1 6c), C 16:0, and C12:0. The cellular quinone content contained exclusively ubiquinone Q-8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The polyamine pattern was composed of putrescine, 2-hydroxputrescine, and spermidine. Article in Journal/Newspaper Antarc* Antarctica James Ross Island Ross Island Brno University of Technology (VUT): Digital Library Ross Island Microbiology Spectrum 9 1 |
institution |
Open Polar |
collection |
Brno University of Technology (VUT): Digital Library |
op_collection_id |
ftunivtbrno |
language |
English |
topic |
psychrophiles Rugamonas taxonomy Antarctica violacein species description |
spellingShingle |
psychrophiles Rugamonas taxonomy Antarctica violacein species description Sedláček, Ivo Holochová, Pavla Sobotka, Roman Busse, Hans-Jürgen Švec, Pavel Králová, Stanislava Šedo, Ondrej Pilný, Jan Staňková, Eva Koublová, Vendula Sedlář, Karel Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov. |
topic_facet |
psychrophiles Rugamonas taxonomy Antarctica violacein species description |
description |
A group of 11 bacterial strains was isolated from streams and lakes located in a deglaciated northern part of James Ross Island, Antarctica. They were rod-shaped, Gram-stain-negative, motile, and catalase-positive and produced blue-violet-pigmented colonies on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, whole-genome sequencing, automated ribotyping, repetitive element sequence-based PCR (rep-PCR), MALDI-TOF MS, fatty acid profile, chemotaxonomy analyses, and extensive biotyping was applied in order to clarify the taxonomic position of these isolates. Phylogenetic analysis based on the 16S rRNA gene indicated that all the isolates constituted a coherent group belonging to the genus Rugamonas. The closest relatives to the representative isolate P5900T were Rugamonas rubra CCM 3730T, Rugamonas rivuli FT103WT, and Rugamonas aquatica FT29WT, exhibiting 99.2%, 99.1%, and 98.6% 16S rRNA pairwise similarity, respectively. The average nucleotide identity and digital DNA-DNA hybridization values calculated from the whole-genome sequencing data clearly proved that P5900T represents a distinct Rugamonas species. The G+C content of genomic DNAs was 66.1mol%. The major components in fatty acid profiles were summed feature 3 (C16:1 7c/C16:1 6c), C 16:0, and C12:0. The cellular quinone content contained exclusively ubiquinone Q-8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The polyamine pattern was composed of putrescine, 2-hydroxputrescine, and spermidine. |
format |
Article in Journal/Newspaper |
author |
Sedláček, Ivo Holochová, Pavla Sobotka, Roman Busse, Hans-Jürgen Švec, Pavel Králová, Stanislava Šedo, Ondrej Pilný, Jan Staňková, Eva Koublová, Vendula Sedlář, Karel |
author_facet |
Sedláček, Ivo Holochová, Pavla Sobotka, Roman Busse, Hans-Jürgen Švec, Pavel Králová, Stanislava Šedo, Ondrej Pilný, Jan Staňková, Eva Koublová, Vendula Sedlář, Karel |
author_sort |
Sedláček, Ivo |
title |
Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov. |
title_short |
Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov. |
title_full |
Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov. |
title_fullStr |
Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov. |
title_full_unstemmed |
Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov. |
title_sort |
classification of a violacein-producing psychrophilic group of isolates associated with freshwater in antarctica and description of rugamonas violacea sp. nov. |
publisher |
American Society for Microbiology |
publishDate |
2021 |
url |
http://hdl.handle.net/11012/201481 https://doi.org/10.1128/Spectrum.00452-21 |
op_coverage |
1 9 |
geographic |
Ross Island |
geographic_facet |
Ross Island |
genre |
Antarc* Antarctica James Ross Island Ross Island |
genre_facet |
Antarc* Antarctica James Ross Island Ross Island |
op_source |
Microbiology spectrum. 2021, vol. 9, issue 1, p. 1-14. |
op_relation |
Microbiology spectrum https://journals.asm.org/doi/10.1128/Spectrum.00452-21 2165-0497 doi:10.1128/Spectrum.00452-21 http://hdl.handle.net/11012/201481 |
op_rights |
Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ openAccess http://www.sherpa.ac.uk/romeo/issn/2165-0497/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1128/Spectrum.00452-21 |
container_title |
Microbiology Spectrum |
container_volume |
9 |
container_issue |
1 |
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1766269808977903616 |