Isolation, amplification, and identification of ancient copepod DNA from lake sediments
Species identification of copepods in lake sediments is often difficult because their remains lack diagnostic features. It is therefore not easy to track changes in copepod biodiversity in lakes through time. We report a method for the isolation, amplification, and identification of copepod DNA from...
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ftunivtasmania:oai:eprints.utas.edu.au:701 2023-05-15T13:42:41+02:00 Isolation, amplification, and identification of ancient copepod DNA from lake sediments Bissett, A Gibson, JAE Jarman, SN Swadling, KM Cromer, L 2005-12 application/pdf https://eprints.utas.edu.au/701/ https://eprints.utas.edu.au/701/1/Bissett.pdf http://aslo.org/lomethods/free/2005/index.html en eng https://eprints.utas.edu.au/701/1/Bissett.pdf Bissett, A, Gibson, JAE, Jarman, SN, Swadling, KM and Cromer, L 2005 , 'Isolation, amplification, and identification of ancient copepod DNA from lake sediments' , Limnology and Oceanography: Methods, vol. 3 , pp. 533-542 . cc_utas 270299 Genetics not elsewhere classified ancient DNA copepod Antarctica lake Boeckella poppei Paralabidocera antarctica Article NonPeerReviewed 2005 ftunivtasmania 2020-05-30T07:14:26Z Species identification of copepods in lake sediments is often difficult because their remains lack diagnostic features. It is therefore not easy to track changes in copepod biodiversity in lakes through time. We report a method for the isolation, amplification, and identification of copepod DNA from whole lake sediments formed in the early Holocene to the present. The method, which involves amplification of a short (~300 base pair) DNA sequence that varies between copepod species, provides a new approach to the study of copepod paleobiodiversity. Successful amplification of copepod DNA was possible in samples as old as 9950 calibrated 14C y BP. Attempts failed to recover DNA from a sediment sample ca 65,000 years old. In most cases the species identified in the sediments matched those of extant lake populations, but analysis of early-mid Holocene sediments from one lake revealed a species that is not present today. We were able to recover copepod DNA from core samples stored at -20 degrees C, at 4 degrees C, and preserved with polyethyleneglycol. Article in Journal/Newspaper Antarc* Antarctica Copepods University of Tasmania: UTas ePrints Boeckella ENVELOPE(-56.999,-56.999,-63.404,-63.404) Lake Boeckella ENVELOPE(-57.000,-57.000,-63.400,-63.400) |
institution |
Open Polar |
collection |
University of Tasmania: UTas ePrints |
op_collection_id |
ftunivtasmania |
language |
English |
topic |
270299 Genetics not elsewhere classified ancient DNA copepod Antarctica lake Boeckella poppei Paralabidocera antarctica |
spellingShingle |
270299 Genetics not elsewhere classified ancient DNA copepod Antarctica lake Boeckella poppei Paralabidocera antarctica Bissett, A Gibson, JAE Jarman, SN Swadling, KM Cromer, L Isolation, amplification, and identification of ancient copepod DNA from lake sediments |
topic_facet |
270299 Genetics not elsewhere classified ancient DNA copepod Antarctica lake Boeckella poppei Paralabidocera antarctica |
description |
Species identification of copepods in lake sediments is often difficult because their remains lack diagnostic features. It is therefore not easy to track changes in copepod biodiversity in lakes through time. We report a method for the isolation, amplification, and identification of copepod DNA from whole lake sediments formed in the early Holocene to the present. The method, which involves amplification of a short (~300 base pair) DNA sequence that varies between copepod species, provides a new approach to the study of copepod paleobiodiversity. Successful amplification of copepod DNA was possible in samples as old as 9950 calibrated 14C y BP. Attempts failed to recover DNA from a sediment sample ca 65,000 years old. In most cases the species identified in the sediments matched those of extant lake populations, but analysis of early-mid Holocene sediments from one lake revealed a species that is not present today. We were able to recover copepod DNA from core samples stored at -20 degrees C, at 4 degrees C, and preserved with polyethyleneglycol. |
format |
Article in Journal/Newspaper |
author |
Bissett, A Gibson, JAE Jarman, SN Swadling, KM Cromer, L |
author_facet |
Bissett, A Gibson, JAE Jarman, SN Swadling, KM Cromer, L |
author_sort |
Bissett, A |
title |
Isolation, amplification, and identification of ancient copepod DNA from lake sediments |
title_short |
Isolation, amplification, and identification of ancient copepod DNA from lake sediments |
title_full |
Isolation, amplification, and identification of ancient copepod DNA from lake sediments |
title_fullStr |
Isolation, amplification, and identification of ancient copepod DNA from lake sediments |
title_full_unstemmed |
Isolation, amplification, and identification of ancient copepod DNA from lake sediments |
title_sort |
isolation, amplification, and identification of ancient copepod dna from lake sediments |
publishDate |
2005 |
url |
https://eprints.utas.edu.au/701/ https://eprints.utas.edu.au/701/1/Bissett.pdf http://aslo.org/lomethods/free/2005/index.html |
long_lat |
ENVELOPE(-56.999,-56.999,-63.404,-63.404) ENVELOPE(-57.000,-57.000,-63.400,-63.400) |
geographic |
Boeckella Lake Boeckella |
geographic_facet |
Boeckella Lake Boeckella |
genre |
Antarc* Antarctica Copepods |
genre_facet |
Antarc* Antarctica Copepods |
op_relation |
https://eprints.utas.edu.au/701/1/Bissett.pdf Bissett, A, Gibson, JAE, Jarman, SN, Swadling, KM and Cromer, L 2005 , 'Isolation, amplification, and identification of ancient copepod DNA from lake sediments' , Limnology and Oceanography: Methods, vol. 3 , pp. 533-542 . |
op_rights |
cc_utas |
_version_ |
1766171768862539776 |