Remarkably coherent population structure for a dominant Antarctic Chlorobium species
Background: In Antarctica, summer sunlight enables phototrophic microorganisms to drive primary production, thereby "feeding" ecosystems to enable their persistence through the long, dark winter months. In Ace Lake, a stratified marine-derived system in the Vestfold Hills of East Antarctic...
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ftunivtasmania:oai:eprints.utas.edu.au:47166 2023-05-15T13:43:28+02:00 Remarkably coherent population structure for a dominant Antarctic Chlorobium species Panwar, P Allen, MA Williams, TJ Haque, S Brazendale, S Hancock, A Paez-Espino, D Cavicchioli, R 2021 https://eprints.utas.edu.au/47166/ unknown BioMed Central Panwar, P, Allen, MA, Williams, TJ, Haque, S, Brazendale, S, Hancock, A, Paez-Espino, D and Cavicchioli, R 2021 , 'Remarkably coherent population structure for a dominant Antarctic Chlorobium species' , Microbiome, vol. 9, no. 1 , pp. 1-23 , doi:10.1186/s40168-021-01173-z <http://dx.doi.org/10.1186/s40168-021-01173-z>. Antarctic microbiology Chlorobi Ecotype Generalist virus Green sulphur bacteria Host-virus interactions Meromictic lake Metagenome-assembled genomes Microbial food web Phylotype Population structure Vitamin B12 Article PeerReviewed 2021 ftunivtasmania https://doi.org/10.1186/s40168-021-01173-z 2022-09-12T22:16:37Z Background: In Antarctica, summer sunlight enables phototrophic microorganisms to drive primary production, thereby "feeding" ecosystems to enable their persistence through the long, dark winter months. In Ace Lake, a stratified marine-derived system in the Vestfold Hills of East Antarctica, a Chlorobium species of green sulphur bacteria (GSB) is the dominant phototroph, although its seasonal abundance changes more than 100-fold. Here, we analysed 413 Gb of Antarctic metagenome data including 59 Chlorobium metagenome-assembled genomes (MAGs) from Ace Lake and nearby stratified marine basins to determine how genome variation and population structure across a 7-year period impacted ecosystem function.Results: A single species, Candidatus Chlorobium antarcticum (most similar to Chlorobium phaeovibrioides DSM265) prevails in all three aquatic systems and harbours very little genomic variation (≥ 99% average nucleotide identity). A notable feature of variation that did exist related to the genomic capacity to biosynthesize cobalamin. The abundance of phylotypes with this capacity changed seasonally ~ 2-fold, consistent with the population balancing the value of a bolstered photosynthetic capacity in summer against an energetic cost in winter. The very high GSB concentration (> 108 cells ml-1 in Ace Lake) and seasonal cycle of cell lysis likely make Ca. Chlorobium antarcticum a major provider of cobalamin to the food web. Analysis of Ca. Chlorobium antarcticum viruses revealed the species to be infected by generalist (rather than specialist) viruses with a broad host range (e.g., infecting Gammaproteobacteria) that were present in diverse Antarctic lakes. The marked seasonal decrease in Ca. Chlorobium antarcticum abundance may restrict specialist viruses from establishing effective lifecycles, whereas generalist viruses may augment their proliferation using other hosts.Conclusion: The factors shaping Antarctic microbial communities are gradually being defined. In addition to the cold, the annual variation in ... Article in Journal/Newspaper Antarc* Antarctic Antarctica East Antarctica University of Tasmania: UTas ePrints Antarctic East Antarctica Vestfold Hills Vestfold Ace Lake ENVELOPE(78.188,78.188,-68.472,-68.472) Microbiome 9 1 |
institution |
Open Polar |
collection |
University of Tasmania: UTas ePrints |
op_collection_id |
ftunivtasmania |
language |
unknown |
topic |
Antarctic microbiology Chlorobi Ecotype Generalist virus Green sulphur bacteria Host-virus interactions Meromictic lake Metagenome-assembled genomes Microbial food web Phylotype Population structure Vitamin B12 |
spellingShingle |
Antarctic microbiology Chlorobi Ecotype Generalist virus Green sulphur bacteria Host-virus interactions Meromictic lake Metagenome-assembled genomes Microbial food web Phylotype Population structure Vitamin B12 Panwar, P Allen, MA Williams, TJ Haque, S Brazendale, S Hancock, A Paez-Espino, D Cavicchioli, R Remarkably coherent population structure for a dominant Antarctic Chlorobium species |
topic_facet |
Antarctic microbiology Chlorobi Ecotype Generalist virus Green sulphur bacteria Host-virus interactions Meromictic lake Metagenome-assembled genomes Microbial food web Phylotype Population structure Vitamin B12 |
description |
Background: In Antarctica, summer sunlight enables phototrophic microorganisms to drive primary production, thereby "feeding" ecosystems to enable their persistence through the long, dark winter months. In Ace Lake, a stratified marine-derived system in the Vestfold Hills of East Antarctica, a Chlorobium species of green sulphur bacteria (GSB) is the dominant phototroph, although its seasonal abundance changes more than 100-fold. Here, we analysed 413 Gb of Antarctic metagenome data including 59 Chlorobium metagenome-assembled genomes (MAGs) from Ace Lake and nearby stratified marine basins to determine how genome variation and population structure across a 7-year period impacted ecosystem function.Results: A single species, Candidatus Chlorobium antarcticum (most similar to Chlorobium phaeovibrioides DSM265) prevails in all three aquatic systems and harbours very little genomic variation (≥ 99% average nucleotide identity). A notable feature of variation that did exist related to the genomic capacity to biosynthesize cobalamin. The abundance of phylotypes with this capacity changed seasonally ~ 2-fold, consistent with the population balancing the value of a bolstered photosynthetic capacity in summer against an energetic cost in winter. The very high GSB concentration (> 108 cells ml-1 in Ace Lake) and seasonal cycle of cell lysis likely make Ca. Chlorobium antarcticum a major provider of cobalamin to the food web. Analysis of Ca. Chlorobium antarcticum viruses revealed the species to be infected by generalist (rather than specialist) viruses with a broad host range (e.g., infecting Gammaproteobacteria) that were present in diverse Antarctic lakes. The marked seasonal decrease in Ca. Chlorobium antarcticum abundance may restrict specialist viruses from establishing effective lifecycles, whereas generalist viruses may augment their proliferation using other hosts.Conclusion: The factors shaping Antarctic microbial communities are gradually being defined. In addition to the cold, the annual variation in ... |
format |
Article in Journal/Newspaper |
author |
Panwar, P Allen, MA Williams, TJ Haque, S Brazendale, S Hancock, A Paez-Espino, D Cavicchioli, R |
author_facet |
Panwar, P Allen, MA Williams, TJ Haque, S Brazendale, S Hancock, A Paez-Espino, D Cavicchioli, R |
author_sort |
Panwar, P |
title |
Remarkably coherent population structure for a dominant Antarctic Chlorobium species |
title_short |
Remarkably coherent population structure for a dominant Antarctic Chlorobium species |
title_full |
Remarkably coherent population structure for a dominant Antarctic Chlorobium species |
title_fullStr |
Remarkably coherent population structure for a dominant Antarctic Chlorobium species |
title_full_unstemmed |
Remarkably coherent population structure for a dominant Antarctic Chlorobium species |
title_sort |
remarkably coherent population structure for a dominant antarctic chlorobium species |
publisher |
BioMed Central |
publishDate |
2021 |
url |
https://eprints.utas.edu.au/47166/ |
long_lat |
ENVELOPE(78.188,78.188,-68.472,-68.472) |
geographic |
Antarctic East Antarctica Vestfold Hills Vestfold Ace Lake |
geographic_facet |
Antarctic East Antarctica Vestfold Hills Vestfold Ace Lake |
genre |
Antarc* Antarctic Antarctica East Antarctica |
genre_facet |
Antarc* Antarctic Antarctica East Antarctica |
op_relation |
Panwar, P, Allen, MA, Williams, TJ, Haque, S, Brazendale, S, Hancock, A, Paez-Espino, D and Cavicchioli, R 2021 , 'Remarkably coherent population structure for a dominant Antarctic Chlorobium species' , Microbiome, vol. 9, no. 1 , pp. 1-23 , doi:10.1186/s40168-021-01173-z <http://dx.doi.org/10.1186/s40168-021-01173-z>. |
op_doi |
https://doi.org/10.1186/s40168-021-01173-z |
container_title |
Microbiome |
container_volume |
9 |
container_issue |
1 |
_version_ |
1766189370043269120 |