Bioavailable iron titrations reveal oceanic Synechococcus ecotypes optimized for different iron availabilities
The trace metal iron (Fe) controls the diversity and activity of phytoplankton across the surface oceans, a paradigm established through decades of in situ and mesocosm experimental studies. Despite widespread Fe-limitation within high-nutrient, low chlorophyll (HNLC) waters, significant contributio...
Published in: | ISME Communications |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Nature Publishing Group
2022
|
Subjects: | |
Online Access: | https://eprints.utas.edu.au/46793/ https://eprints.utas.edu.au/46793/1/150903%20-%20Bioavailable%20iron%20titrations%20reveal%20oceanic%20Synechococcus.pdf |
id |
ftunivtasmania:oai:eprints.utas.edu.au:46793 |
---|---|
record_format |
openpolar |
spelling |
ftunivtasmania:oai:eprints.utas.edu.au:46793 2023-05-15T13:43:28+02:00 Bioavailable iron titrations reveal oceanic Synechococcus ecotypes optimized for different iron availabilities Gilbert, NE LeCleir, GR Strzepek, RF Ellwood, MJ Twining, BS Roux, S Pennacchio, C Boyd, PW Wilhelm, SW 2022 application/pdf https://eprints.utas.edu.au/46793/ https://eprints.utas.edu.au/46793/1/150903%20-%20Bioavailable%20iron%20titrations%20reveal%20oceanic%20Synechococcus.pdf en eng Nature Publishing Group https://eprints.utas.edu.au/46793/1/150903%20-%20Bioavailable%20iron%20titrations%20reveal%20oceanic%20Synechococcus.pdf Gilbert, NE, LeCleir, GR, Strzepek, RF orcid:0000-0002-6442-7121 , Ellwood, MJ, Twining, BS, Roux, S, Pennacchio, C, Boyd, PW orcid:0000-0001-7850-1911 and Wilhelm, SW 2022 , 'Bioavailable iron titrations reveal oceanic Synechococcus ecotypes optimized for different iron availabilities' , ISME Communications, vol. 2 , pp. 1-12 , doi:10.1038/s43705-022-00132-5 <http://dx.doi.org/10.1038/s43705-022-00132-5>. iron Southern Ocean cyanobacteria ecotype physiology transcriptomics Article PeerReviewed 2022 ftunivtasmania https://doi.org/10.1038/s43705-022-00132-5 2022-08-15T22:16:34Z The trace metal iron (Fe) controls the diversity and activity of phytoplankton across the surface oceans, a paradigm established through decades of in situ and mesocosm experimental studies. Despite widespread Fe-limitation within high-nutrient, low chlorophyll (HNLC) waters, significant contributions of the cyanobacterium Synechococcus to the phytoplankton stock can be found. Correlations among differing strains of Synechococcus across different Fe-regimes have suggested the existence of Fe-adapted ecotypes. However, experimental evidence of high- versus low-Fe adapted strains of Synechococcus is lacking, and so we investigated the transcriptional responses of microbial communities inhabiting the HNLC, sub-Antarctic region of the Southern Ocean during the Spring of 2018. Analysis of metatranscriptomes generated from on-deck incubation experiments reflecting a gradient of Fe-availabilities reveal transcriptomic signatures indicative of co-occurring Synechococcus ecotypes adapted to differing Fe-regimes. Functional analyses comparing low-Fe and high-Fe conditions point to various Fe-acquisition mechanisms that may allow persistence of low-Fe adapted Synechococcus under Fe-limitation. Comparison of in situ surface conditions to the Fe-titrations indicate ecological relevance of these mechanisms as well as persistence of both putative ecotypes within this region. This Fe-titration approach, combined with transcriptomics, highlights the short-term responses of the in situ phytoplankton community to Fe-availability that are often overlooked by examining genomic content or bulk physiological responses alone. These findings expand our knowledge about how phytoplankton in HNLC Southern Ocean waters adapt and respond to changing Fe supply. Article in Journal/Newspaper Antarc* Antarctic Southern Ocean University of Tasmania: UTas ePrints Antarctic Southern Ocean ISME Communications 2 1 |
institution |
Open Polar |
collection |
University of Tasmania: UTas ePrints |
op_collection_id |
ftunivtasmania |
language |
English |
topic |
iron Southern Ocean cyanobacteria ecotype physiology transcriptomics |
spellingShingle |
iron Southern Ocean cyanobacteria ecotype physiology transcriptomics Gilbert, NE LeCleir, GR Strzepek, RF Ellwood, MJ Twining, BS Roux, S Pennacchio, C Boyd, PW Wilhelm, SW Bioavailable iron titrations reveal oceanic Synechococcus ecotypes optimized for different iron availabilities |
topic_facet |
iron Southern Ocean cyanobacteria ecotype physiology transcriptomics |
description |
The trace metal iron (Fe) controls the diversity and activity of phytoplankton across the surface oceans, a paradigm established through decades of in situ and mesocosm experimental studies. Despite widespread Fe-limitation within high-nutrient, low chlorophyll (HNLC) waters, significant contributions of the cyanobacterium Synechococcus to the phytoplankton stock can be found. Correlations among differing strains of Synechococcus across different Fe-regimes have suggested the existence of Fe-adapted ecotypes. However, experimental evidence of high- versus low-Fe adapted strains of Synechococcus is lacking, and so we investigated the transcriptional responses of microbial communities inhabiting the HNLC, sub-Antarctic region of the Southern Ocean during the Spring of 2018. Analysis of metatranscriptomes generated from on-deck incubation experiments reflecting a gradient of Fe-availabilities reveal transcriptomic signatures indicative of co-occurring Synechococcus ecotypes adapted to differing Fe-regimes. Functional analyses comparing low-Fe and high-Fe conditions point to various Fe-acquisition mechanisms that may allow persistence of low-Fe adapted Synechococcus under Fe-limitation. Comparison of in situ surface conditions to the Fe-titrations indicate ecological relevance of these mechanisms as well as persistence of both putative ecotypes within this region. This Fe-titration approach, combined with transcriptomics, highlights the short-term responses of the in situ phytoplankton community to Fe-availability that are often overlooked by examining genomic content or bulk physiological responses alone. These findings expand our knowledge about how phytoplankton in HNLC Southern Ocean waters adapt and respond to changing Fe supply. |
format |
Article in Journal/Newspaper |
author |
Gilbert, NE LeCleir, GR Strzepek, RF Ellwood, MJ Twining, BS Roux, S Pennacchio, C Boyd, PW Wilhelm, SW |
author_facet |
Gilbert, NE LeCleir, GR Strzepek, RF Ellwood, MJ Twining, BS Roux, S Pennacchio, C Boyd, PW Wilhelm, SW |
author_sort |
Gilbert, NE |
title |
Bioavailable iron titrations reveal oceanic Synechococcus ecotypes optimized for different iron availabilities |
title_short |
Bioavailable iron titrations reveal oceanic Synechococcus ecotypes optimized for different iron availabilities |
title_full |
Bioavailable iron titrations reveal oceanic Synechococcus ecotypes optimized for different iron availabilities |
title_fullStr |
Bioavailable iron titrations reveal oceanic Synechococcus ecotypes optimized for different iron availabilities |
title_full_unstemmed |
Bioavailable iron titrations reveal oceanic Synechococcus ecotypes optimized for different iron availabilities |
title_sort |
bioavailable iron titrations reveal oceanic synechococcus ecotypes optimized for different iron availabilities |
publisher |
Nature Publishing Group |
publishDate |
2022 |
url |
https://eprints.utas.edu.au/46793/ https://eprints.utas.edu.au/46793/1/150903%20-%20Bioavailable%20iron%20titrations%20reveal%20oceanic%20Synechococcus.pdf |
geographic |
Antarctic Southern Ocean |
geographic_facet |
Antarctic Southern Ocean |
genre |
Antarc* Antarctic Southern Ocean |
genre_facet |
Antarc* Antarctic Southern Ocean |
op_relation |
https://eprints.utas.edu.au/46793/1/150903%20-%20Bioavailable%20iron%20titrations%20reveal%20oceanic%20Synechococcus.pdf Gilbert, NE, LeCleir, GR, Strzepek, RF orcid:0000-0002-6442-7121 , Ellwood, MJ, Twining, BS, Roux, S, Pennacchio, C, Boyd, PW orcid:0000-0001-7850-1911 and Wilhelm, SW 2022 , 'Bioavailable iron titrations reveal oceanic Synechococcus ecotypes optimized for different iron availabilities' , ISME Communications, vol. 2 , pp. 1-12 , doi:10.1038/s43705-022-00132-5 <http://dx.doi.org/10.1038/s43705-022-00132-5>. |
op_doi |
https://doi.org/10.1038/s43705-022-00132-5 |
container_title |
ISME Communications |
container_volume |
2 |
container_issue |
1 |
_version_ |
1766189325411680256 |