Repeated sublethal freshwater exposures reduce the amoebic gill disease parasite, Neoparamoeba perurans, on Atlantic salmon
Freshwater bathing is one of the main treatment options available against amoebic gill disease (AGD) affecting multiple fish hosts in mariculture systems. Prevailing freshwater treatments are designed to be long enough to kill Neoparamoeba perurans, the ectoparasite causing AGD, which may select for...
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Blackwell Publishing Ltd
2018
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Online Access: | https://eprints.utas.edu.au/45192/ |
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ftunivtasmania:oai:eprints.utas.edu.au:45192 2023-05-15T15:31:23+02:00 Repeated sublethal freshwater exposures reduce the amoebic gill disease parasite, Neoparamoeba perurans, on Atlantic salmon Wright, DW Nowak, B Oppedal, F Crosbie, P Stien, LH Dempster, T 2018 https://eprints.utas.edu.au/45192/ unknown Blackwell Publishing Ltd Wright, DW, Nowak, B orcid:0000-0002-0347-643X , Oppedal, F, Crosbie, P orcid:0000-0001-8856-1731 , Stien, LH and Dempster, T 2018 , 'Repeated sublethal freshwater exposures reduce the amoebic gill disease parasite, Neoparamoeba perurans, on Atlantic salmon' , Journal of Fish Diseases, vol. 41, no. 9 , pp. 1403-1410 , doi:10.1111/jfd.12834 <http://dx.doi.org/10.1111/jfd.12834>. freshwater mariculture moderate vs aggressive treatment Neoparamoeba perurans parasite control Article PeerReviewed 2018 ftunivtasmania https://doi.org/10.1111/jfd.12834 2022-03-07T23:17:12Z Freshwater bathing is one of the main treatment options available against amoebic gill disease (AGD) affecting multiple fish hosts in mariculture systems. Prevailing freshwater treatments are designed to be long enough to kill Neoparamoeba perurans, the ectoparasite causing AGD, which may select for freshwater tolerance. Here, we tested whether using shorter, sublethal freshwater treatment durations are a viable alternative to lethal ones for N. perurans (2–4 hr). Under in vitro conditions, gill‐isolated N. perurans attached to plastic substrate in sea water lifted off after ≥2 min in freshwater, but survival was not impacted until 60 min. In an in vivo experiment, AGD‐affected Atlantic salmon Salmo salar subjected daily to 30 min (sublethal to N. perurans) and 120 min (lethal to N. perurans) freshwater treatments for 6 days consistently reduced N. perurans cell numbers on gills (based on qPCR analysis) compared to daily 3 min freshwater or seawater treatments for 6 days. Our results suggest that targeting cell detachment rather than cell death with repeated freshwater treatments of shorter duration than typical baths could be used in AGD management. However, the consequences of modifying the intensity of freshwater treatment regimes on freshwater tolerance evolution in N. perurans populations require careful consideration. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Tasmania: UTas ePrints Journal of Fish Diseases 41 9 1403 1410 |
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Open Polar |
collection |
University of Tasmania: UTas ePrints |
op_collection_id |
ftunivtasmania |
language |
unknown |
topic |
freshwater mariculture moderate vs aggressive treatment Neoparamoeba perurans parasite control |
spellingShingle |
freshwater mariculture moderate vs aggressive treatment Neoparamoeba perurans parasite control Wright, DW Nowak, B Oppedal, F Crosbie, P Stien, LH Dempster, T Repeated sublethal freshwater exposures reduce the amoebic gill disease parasite, Neoparamoeba perurans, on Atlantic salmon |
topic_facet |
freshwater mariculture moderate vs aggressive treatment Neoparamoeba perurans parasite control |
description |
Freshwater bathing is one of the main treatment options available against amoebic gill disease (AGD) affecting multiple fish hosts in mariculture systems. Prevailing freshwater treatments are designed to be long enough to kill Neoparamoeba perurans, the ectoparasite causing AGD, which may select for freshwater tolerance. Here, we tested whether using shorter, sublethal freshwater treatment durations are a viable alternative to lethal ones for N. perurans (2–4 hr). Under in vitro conditions, gill‐isolated N. perurans attached to plastic substrate in sea water lifted off after ≥2 min in freshwater, but survival was not impacted until 60 min. In an in vivo experiment, AGD‐affected Atlantic salmon Salmo salar subjected daily to 30 min (sublethal to N. perurans) and 120 min (lethal to N. perurans) freshwater treatments for 6 days consistently reduced N. perurans cell numbers on gills (based on qPCR analysis) compared to daily 3 min freshwater or seawater treatments for 6 days. Our results suggest that targeting cell detachment rather than cell death with repeated freshwater treatments of shorter duration than typical baths could be used in AGD management. However, the consequences of modifying the intensity of freshwater treatment regimes on freshwater tolerance evolution in N. perurans populations require careful consideration. |
format |
Article in Journal/Newspaper |
author |
Wright, DW Nowak, B Oppedal, F Crosbie, P Stien, LH Dempster, T |
author_facet |
Wright, DW Nowak, B Oppedal, F Crosbie, P Stien, LH Dempster, T |
author_sort |
Wright, DW |
title |
Repeated sublethal freshwater exposures reduce the amoebic gill disease parasite, Neoparamoeba perurans, on Atlantic salmon |
title_short |
Repeated sublethal freshwater exposures reduce the amoebic gill disease parasite, Neoparamoeba perurans, on Atlantic salmon |
title_full |
Repeated sublethal freshwater exposures reduce the amoebic gill disease parasite, Neoparamoeba perurans, on Atlantic salmon |
title_fullStr |
Repeated sublethal freshwater exposures reduce the amoebic gill disease parasite, Neoparamoeba perurans, on Atlantic salmon |
title_full_unstemmed |
Repeated sublethal freshwater exposures reduce the amoebic gill disease parasite, Neoparamoeba perurans, on Atlantic salmon |
title_sort |
repeated sublethal freshwater exposures reduce the amoebic gill disease parasite, neoparamoeba perurans, on atlantic salmon |
publisher |
Blackwell Publishing Ltd |
publishDate |
2018 |
url |
https://eprints.utas.edu.au/45192/ |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Wright, DW, Nowak, B orcid:0000-0002-0347-643X , Oppedal, F, Crosbie, P orcid:0000-0001-8856-1731 , Stien, LH and Dempster, T 2018 , 'Repeated sublethal freshwater exposures reduce the amoebic gill disease parasite, Neoparamoeba perurans, on Atlantic salmon' , Journal of Fish Diseases, vol. 41, no. 9 , pp. 1403-1410 , doi:10.1111/jfd.12834 <http://dx.doi.org/10.1111/jfd.12834>. |
op_doi |
https://doi.org/10.1111/jfd.12834 |
container_title |
Journal of Fish Diseases |
container_volume |
41 |
container_issue |
9 |
container_start_page |
1403 |
op_container_end_page |
1410 |
_version_ |
1766361881547636736 |