Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD)
Amoebic gill disease (AGD) is one of the main health issues impacting farmed Atlantic salmon. Neoparamoeba perurans causes AGD; however, a diversity of other amoeba species colonizes the gills and there is little understanding of whether they are commensal or potentially involved in different stages...
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Blackwell Publishing Ltd
2021
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ftunivtasmania:oai:eprints.utas.edu.au:44180 2023-05-15T15:30:08+02:00 Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD) English, CJ Botwright, NA Adams, MB Barnes, AC Wynne, JW Lima, PC Cook, MT 2021 https://eprints.utas.edu.au/44180/ unknown Blackwell Publishing Ltd English, CJ, Botwright, NA, Adams, MB orcid:0000-0002-5737-5474 , Barnes, AC, Wynne, JW, Lima, PC and Cook, MT 2021 , 'Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD)' , Journal of Fish Diseases, vol. 44, no. 2 , pp. 149-160 , doi:10.1111/jfd.13319 <http://dx.doi.org/10.1111/jfd.13319>. etiology Amoebozoa aquaculture gill disease histopathology Article PeerReviewed 2021 ftunivtasmania https://doi.org/10.1111/jfd.13319 2022-02-28T23:16:48Z Amoebic gill disease (AGD) is one of the main health issues impacting farmed Atlantic salmon. Neoparamoeba perurans causes AGD; however, a diversity of other amoeba species colonizes the gills and there is little understanding of whether they are commensal or potentially involved in different stages of gill disease development. Here, we conduct in vivo challenges of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. to investigate their pathogenicity to Atlantic salmon gills. Additionally, we assessed whether the presence of Nolandella sp. and Pseudoparamoeba sp. influences the onset and/or severity of N. perurans‐induced AGD. All three strains attached and multiplied on the gills according to qPCR analysis. Furthermore, minor gross gill lesions and histological changes were observed post‐exposure. While N. perurans was found associated with classical AGD lesions, Nolandella sp. and Pseudoparamoeba sp. were not found associated with lesion sites and these lesions did not meet the expected composite of histopathological changes for AGD. Moreover, the presence of these non‐N. perurans species did not significantly increase the severity of AGD. This trial provides evidence that cultured Nolandella sp. and Pseudoparamoeba sp. do not induce AGD and do not influence the severity of AGD during the early stages of development. Article in Journal/Newspaper Atlantic salmon University of Tasmania: UTas ePrints Journal of Fish Diseases 44 2 149 160 |
institution |
Open Polar |
collection |
University of Tasmania: UTas ePrints |
op_collection_id |
ftunivtasmania |
language |
unknown |
topic |
etiology Amoebozoa aquaculture gill disease histopathology |
spellingShingle |
etiology Amoebozoa aquaculture gill disease histopathology English, CJ Botwright, NA Adams, MB Barnes, AC Wynne, JW Lima, PC Cook, MT Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD) |
topic_facet |
etiology Amoebozoa aquaculture gill disease histopathology |
description |
Amoebic gill disease (AGD) is one of the main health issues impacting farmed Atlantic salmon. Neoparamoeba perurans causes AGD; however, a diversity of other amoeba species colonizes the gills and there is little understanding of whether they are commensal or potentially involved in different stages of gill disease development. Here, we conduct in vivo challenges of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. to investigate their pathogenicity to Atlantic salmon gills. Additionally, we assessed whether the presence of Nolandella sp. and Pseudoparamoeba sp. influences the onset and/or severity of N. perurans‐induced AGD. All three strains attached and multiplied on the gills according to qPCR analysis. Furthermore, minor gross gill lesions and histological changes were observed post‐exposure. While N. perurans was found associated with classical AGD lesions, Nolandella sp. and Pseudoparamoeba sp. were not found associated with lesion sites and these lesions did not meet the expected composite of histopathological changes for AGD. Moreover, the presence of these non‐N. perurans species did not significantly increase the severity of AGD. This trial provides evidence that cultured Nolandella sp. and Pseudoparamoeba sp. do not induce AGD and do not influence the severity of AGD during the early stages of development. |
format |
Article in Journal/Newspaper |
author |
English, CJ Botwright, NA Adams, MB Barnes, AC Wynne, JW Lima, PC Cook, MT |
author_facet |
English, CJ Botwright, NA Adams, MB Barnes, AC Wynne, JW Lima, PC Cook, MT |
author_sort |
English, CJ |
title |
Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD) |
title_short |
Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD) |
title_full |
Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD) |
title_fullStr |
Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD) |
title_full_unstemmed |
Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD) |
title_sort |
immersion challenge of naïve atlantic salmon with cultured nolandella sp. and pseudoparamoeba sp. did not increase the severity of neoparamoeba perurans-induced amoebic gill disease (agd) |
publisher |
Blackwell Publishing Ltd |
publishDate |
2021 |
url |
https://eprints.utas.edu.au/44180/ |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
English, CJ, Botwright, NA, Adams, MB orcid:0000-0002-5737-5474 , Barnes, AC, Wynne, JW, Lima, PC and Cook, MT 2021 , 'Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD)' , Journal of Fish Diseases, vol. 44, no. 2 , pp. 149-160 , doi:10.1111/jfd.13319 <http://dx.doi.org/10.1111/jfd.13319>. |
op_doi |
https://doi.org/10.1111/jfd.13319 |
container_title |
Journal of Fish Diseases |
container_volume |
44 |
container_issue |
2 |
container_start_page |
149 |
op_container_end_page |
160 |
_version_ |
1766360574275354624 |