Bacteriomic profiling of branchial lesions induced by Neoparamoeba perurans challenge reveals commensal dysbiosis and an association with Tenacibaculum dicentrarchi in AGD-affected Atlantic salmon (Salmo Salar L.)
Amoebic gill disease is a parasitic condition that commonly affects marine farmed Atlantic salmon. The causative agent, Neoparamoeba perurans, induces a marked proliferation of the gill mucosa and focal superficial necrosis upon branchial lesions. The effect that amoebic branchialitis has upon gill...
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ftunivtasmania:oai:eprints.utas.edu.au:43947 2023-05-15T15:30:35+02:00 Bacteriomic profiling of branchial lesions induced by Neoparamoeba perurans challenge reveals commensal dysbiosis and an association with Tenacibaculum dicentrarchi in AGD-affected Atlantic salmon (Salmo Salar L.) Slinger, J Adams, MB Wynne, JW 2020 application/pdf https://eprints.utas.edu.au/43947/ https://eprints.utas.edu.au/43947/1/140298%20-%20Bacteriomic%20profiling%20of%20branchial%20lesions%20induced%20by%20Neoparamoeba%20perurans.pdf en eng MDPIAG https://eprints.utas.edu.au/43947/1/140298%20-%20Bacteriomic%20profiling%20of%20branchial%20lesions%20induced%20by%20Neoparamoeba%20perurans.pdf Slinger, J, Adams, MB orcid:0000-0002-5737-5474 and Wynne, JW 2020 , 'Bacteriomic profiling of branchial lesions induced by Neoparamoeba perurans challenge reveals commensal dysbiosis and an association with Tenacibaculum dicentrarchi in AGD-affected Atlantic salmon (Salmo Salar L.)' , Microorganisms, vol. 8, no. 8 , pp. 1-17 , doi:10.3390/microorganisms8081189 <http://dx.doi.org/10.3390/microorganisms8081189>. AGD microbiomics gills amoeba Neoparamoeba bacteria Atlantic salmon bacteriome gill microbiota Tenacibaculum Tenacibaculosis Article PeerReviewed 2020 ftunivtasmania https://doi.org/10.3390/microorganisms8081189 2022-02-21T23:18:29Z Amoebic gill disease is a parasitic condition that commonly affects marine farmed Atlantic salmon. The causative agent, Neoparamoeba perurans, induces a marked proliferation of the gill mucosa and focal superficial necrosis upon branchial lesions. The effect that amoebic branchialitis has upon gill associated commensal bacteria is unknown. A 16S rRNA sequencing approach was employed to profile changes in bacterial community composition, within amoebic gill disease (AGD)-affected and non-affected gill tissue. The bacterial diversity of biopsies with and without diseased tissue was significantly lower in the AGD-affected fish compared to uninfected fish. Furthermore, within the AGD-affected tissue, lesions appeared to contain a significantly higher abundance of the Flavobacterium, Tenacibaculum dicentrarchi compared to adjunct unaffected tissues. Quantitative PCR specific to both N. perurans and T. dicentrarchi was used to further examine the co-abundance of these known fish pathogens. A moderate positive correlation between these pathogens was observed. Taken together, the present study sheds new light on the complex interaction between the host, parasite and bacterial communities during AGD progression. The role that T. dicentrarchi may play in this complex relationship requires further investigation. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Tasmania: UTas ePrints Microorganisms 8 8 1189 |
institution |
Open Polar |
collection |
University of Tasmania: UTas ePrints |
op_collection_id |
ftunivtasmania |
language |
English |
topic |
AGD microbiomics gills amoeba Neoparamoeba bacteria Atlantic salmon bacteriome gill microbiota Tenacibaculum Tenacibaculosis |
spellingShingle |
AGD microbiomics gills amoeba Neoparamoeba bacteria Atlantic salmon bacteriome gill microbiota Tenacibaculum Tenacibaculosis Slinger, J Adams, MB Wynne, JW Bacteriomic profiling of branchial lesions induced by Neoparamoeba perurans challenge reveals commensal dysbiosis and an association with Tenacibaculum dicentrarchi in AGD-affected Atlantic salmon (Salmo Salar L.) |
topic_facet |
AGD microbiomics gills amoeba Neoparamoeba bacteria Atlantic salmon bacteriome gill microbiota Tenacibaculum Tenacibaculosis |
description |
Amoebic gill disease is a parasitic condition that commonly affects marine farmed Atlantic salmon. The causative agent, Neoparamoeba perurans, induces a marked proliferation of the gill mucosa and focal superficial necrosis upon branchial lesions. The effect that amoebic branchialitis has upon gill associated commensal bacteria is unknown. A 16S rRNA sequencing approach was employed to profile changes in bacterial community composition, within amoebic gill disease (AGD)-affected and non-affected gill tissue. The bacterial diversity of biopsies with and without diseased tissue was significantly lower in the AGD-affected fish compared to uninfected fish. Furthermore, within the AGD-affected tissue, lesions appeared to contain a significantly higher abundance of the Flavobacterium, Tenacibaculum dicentrarchi compared to adjunct unaffected tissues. Quantitative PCR specific to both N. perurans and T. dicentrarchi was used to further examine the co-abundance of these known fish pathogens. A moderate positive correlation between these pathogens was observed. Taken together, the present study sheds new light on the complex interaction between the host, parasite and bacterial communities during AGD progression. The role that T. dicentrarchi may play in this complex relationship requires further investigation. |
format |
Article in Journal/Newspaper |
author |
Slinger, J Adams, MB Wynne, JW |
author_facet |
Slinger, J Adams, MB Wynne, JW |
author_sort |
Slinger, J |
title |
Bacteriomic profiling of branchial lesions induced by Neoparamoeba perurans challenge reveals commensal dysbiosis and an association with Tenacibaculum dicentrarchi in AGD-affected Atlantic salmon (Salmo Salar L.) |
title_short |
Bacteriomic profiling of branchial lesions induced by Neoparamoeba perurans challenge reveals commensal dysbiosis and an association with Tenacibaculum dicentrarchi in AGD-affected Atlantic salmon (Salmo Salar L.) |
title_full |
Bacteriomic profiling of branchial lesions induced by Neoparamoeba perurans challenge reveals commensal dysbiosis and an association with Tenacibaculum dicentrarchi in AGD-affected Atlantic salmon (Salmo Salar L.) |
title_fullStr |
Bacteriomic profiling of branchial lesions induced by Neoparamoeba perurans challenge reveals commensal dysbiosis and an association with Tenacibaculum dicentrarchi in AGD-affected Atlantic salmon (Salmo Salar L.) |
title_full_unstemmed |
Bacteriomic profiling of branchial lesions induced by Neoparamoeba perurans challenge reveals commensal dysbiosis and an association with Tenacibaculum dicentrarchi in AGD-affected Atlantic salmon (Salmo Salar L.) |
title_sort |
bacteriomic profiling of branchial lesions induced by neoparamoeba perurans challenge reveals commensal dysbiosis and an association with tenacibaculum dicentrarchi in agd-affected atlantic salmon (salmo salar l.) |
publisher |
MDPIAG |
publishDate |
2020 |
url |
https://eprints.utas.edu.au/43947/ https://eprints.utas.edu.au/43947/1/140298%20-%20Bacteriomic%20profiling%20of%20branchial%20lesions%20induced%20by%20Neoparamoeba%20perurans.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://eprints.utas.edu.au/43947/1/140298%20-%20Bacteriomic%20profiling%20of%20branchial%20lesions%20induced%20by%20Neoparamoeba%20perurans.pdf Slinger, J, Adams, MB orcid:0000-0002-5737-5474 and Wynne, JW 2020 , 'Bacteriomic profiling of branchial lesions induced by Neoparamoeba perurans challenge reveals commensal dysbiosis and an association with Tenacibaculum dicentrarchi in AGD-affected Atlantic salmon (Salmo Salar L.)' , Microorganisms, vol. 8, no. 8 , pp. 1-17 , doi:10.3390/microorganisms8081189 <http://dx.doi.org/10.3390/microorganisms8081189>. |
op_doi |
https://doi.org/10.3390/microorganisms8081189 |
container_title |
Microorganisms |
container_volume |
8 |
container_issue |
8 |
container_start_page |
1189 |
_version_ |
1766361043317030912 |