Dark metabolism: a molecular insight into how the Antarctic sea-ice diatom Fragilariopsis cylindrus survives long-term darkness
Light underneath Antarctic sea‐ice is below detectable limits for up to 4 months of the year. The ability of Antarctic sea‐ice diatoms to survive this prolonged darkness relies on their metabolic capability. This study is the first to examine the proteome of a prominent sea‐ice diatom in response to...
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Blackwell Publishing Ltd
2019
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Online Access: | https://eprints.utas.edu.au/41620/ https://eprints.utas.edu.au/41620/1/132747-Dark%20metabolism_a%20molecular%20insight.pdf |
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ftunivtasmania:oai:eprints.utas.edu.au:41620 2023-05-15T13:41:50+02:00 Dark metabolism: a molecular insight into how the Antarctic sea-ice diatom Fragilariopsis cylindrus survives long-term darkness Kennedy, F Martin, A Bowman, JP Wilson, R McMinn, A 2019 application/pdf https://eprints.utas.edu.au/41620/ https://eprints.utas.edu.au/41620/1/132747-Dark%20metabolism_a%20molecular%20insight.pdf en eng Blackwell Publishing Ltd https://eprints.utas.edu.au/41620/1/132747-Dark%20metabolism_a%20molecular%20insight.pdf Kennedy, F orcid:0000-0003-1796-0764 , Martin, A orcid:0000-0001-8260-5529 , Bowman, JP orcid:0000-0002-4528-9333 , Wilson, R orcid:0000-0003-0152-4394 and McMinn, A orcid:0000-0002-2133-3854 2019 , 'Dark metabolism: a molecular insight into how the Antarctic sea-ice diatom Fragilariopsis cylindrus survives long-term darkness' , New Phytologist, vol. 223, no. 2 , pp. 675-691 , doi:10.1111/nph.15843 <http://dx.doi.org/10.1111/nph.15843>. Antarctic dark survival Fragilariopsis cylindrus metabolism proteomics sea-ice algae Article PeerReviewed 2019 ftunivtasmania https://doi.org/10.1111/nph.15843 2022-01-31T23:18:26Z Light underneath Antarctic sea‐ice is below detectable limits for up to 4 months of the year. The ability of Antarctic sea‐ice diatoms to survive this prolonged darkness relies on their metabolic capability. This study is the first to examine the proteome of a prominent sea‐ice diatom in response to extended darkness, focusing on the protein‐level mechanisms of dark survival.The Antarctic diatom Fragilariopsis cylindrus was grown under continuous light or darkness for 120 d. The whole cell proteome was quantitatively analysed by nano‐LC−MS/MS to investigate metabolic changes that occur during sustained darkness and during recovery under illumination.Enzymes of metabolic pathways, particularly those involved in respiratory processes, tricarboxylic acid cycle, glycolysis, the Entner−Doudoroff pathway, the urea cycle and the mitochondrial electron transport chain became more abundant in the dark. Within the plastid, carbon fixation halted while the upper sections of the glycolysis, gluconeogenesis and pentose phosphate pathways became less active.We have discovered how F. cylindrus utilises an ancient alternative metabolic mechanism that enables its capacity for long‐term dark survival. By sustaining essential metabolic processes in the dark, F. cylindrus retains the functionality of the photosynthetic apparatus, ensuring rapid recovery upon re‐illumination. Article in Journal/Newspaper Antarc* Antarctic ice algae Sea ice University of Tasmania: UTas ePrints Antarctic The Antarctic New Phytologist 223 2 675 691 |
institution |
Open Polar |
collection |
University of Tasmania: UTas ePrints |
op_collection_id |
ftunivtasmania |
language |
English |
topic |
Antarctic dark survival Fragilariopsis cylindrus metabolism proteomics sea-ice algae |
spellingShingle |
Antarctic dark survival Fragilariopsis cylindrus metabolism proteomics sea-ice algae Kennedy, F Martin, A Bowman, JP Wilson, R McMinn, A Dark metabolism: a molecular insight into how the Antarctic sea-ice diatom Fragilariopsis cylindrus survives long-term darkness |
topic_facet |
Antarctic dark survival Fragilariopsis cylindrus metabolism proteomics sea-ice algae |
description |
Light underneath Antarctic sea‐ice is below detectable limits for up to 4 months of the year. The ability of Antarctic sea‐ice diatoms to survive this prolonged darkness relies on their metabolic capability. This study is the first to examine the proteome of a prominent sea‐ice diatom in response to extended darkness, focusing on the protein‐level mechanisms of dark survival.The Antarctic diatom Fragilariopsis cylindrus was grown under continuous light or darkness for 120 d. The whole cell proteome was quantitatively analysed by nano‐LC−MS/MS to investigate metabolic changes that occur during sustained darkness and during recovery under illumination.Enzymes of metabolic pathways, particularly those involved in respiratory processes, tricarboxylic acid cycle, glycolysis, the Entner−Doudoroff pathway, the urea cycle and the mitochondrial electron transport chain became more abundant in the dark. Within the plastid, carbon fixation halted while the upper sections of the glycolysis, gluconeogenesis and pentose phosphate pathways became less active.We have discovered how F. cylindrus utilises an ancient alternative metabolic mechanism that enables its capacity for long‐term dark survival. By sustaining essential metabolic processes in the dark, F. cylindrus retains the functionality of the photosynthetic apparatus, ensuring rapid recovery upon re‐illumination. |
format |
Article in Journal/Newspaper |
author |
Kennedy, F Martin, A Bowman, JP Wilson, R McMinn, A |
author_facet |
Kennedy, F Martin, A Bowman, JP Wilson, R McMinn, A |
author_sort |
Kennedy, F |
title |
Dark metabolism: a molecular insight into how the Antarctic sea-ice diatom Fragilariopsis cylindrus survives long-term darkness |
title_short |
Dark metabolism: a molecular insight into how the Antarctic sea-ice diatom Fragilariopsis cylindrus survives long-term darkness |
title_full |
Dark metabolism: a molecular insight into how the Antarctic sea-ice diatom Fragilariopsis cylindrus survives long-term darkness |
title_fullStr |
Dark metabolism: a molecular insight into how the Antarctic sea-ice diatom Fragilariopsis cylindrus survives long-term darkness |
title_full_unstemmed |
Dark metabolism: a molecular insight into how the Antarctic sea-ice diatom Fragilariopsis cylindrus survives long-term darkness |
title_sort |
dark metabolism: a molecular insight into how the antarctic sea-ice diatom fragilariopsis cylindrus survives long-term darkness |
publisher |
Blackwell Publishing Ltd |
publishDate |
2019 |
url |
https://eprints.utas.edu.au/41620/ https://eprints.utas.edu.au/41620/1/132747-Dark%20metabolism_a%20molecular%20insight.pdf |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic ice algae Sea ice |
genre_facet |
Antarc* Antarctic ice algae Sea ice |
op_relation |
https://eprints.utas.edu.au/41620/1/132747-Dark%20metabolism_a%20molecular%20insight.pdf Kennedy, F orcid:0000-0003-1796-0764 , Martin, A orcid:0000-0001-8260-5529 , Bowman, JP orcid:0000-0002-4528-9333 , Wilson, R orcid:0000-0003-0152-4394 and McMinn, A orcid:0000-0002-2133-3854 2019 , 'Dark metabolism: a molecular insight into how the Antarctic sea-ice diatom Fragilariopsis cylindrus survives long-term darkness' , New Phytologist, vol. 223, no. 2 , pp. 675-691 , doi:10.1111/nph.15843 <http://dx.doi.org/10.1111/nph.15843>. |
op_doi |
https://doi.org/10.1111/nph.15843 |
container_title |
New Phytologist |
container_volume |
223 |
container_issue |
2 |
container_start_page |
675 |
op_container_end_page |
691 |
_version_ |
1766158666261594112 |