Differences in diversity and photoprotection capability between ice algae and under-ice phytoplankton in Saroma-Ko Lagoon, Japan: a comparative taxonomic diatom analysis with microscopy and DNA barcoding
Sea ice algae, comprised mainly of diatoms, are the main primary producers in polar ecosystems, and they are generally distributed with the highest biomass at the bottom of ice. The taxonomy of ice algae has been traditionally investigated using light microscopy, but molecular techniques, including...
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ftunivtasmania:oai:eprints.utas.edu.au:35311 2023-05-15T16:36:32+02:00 Differences in diversity and photoprotection capability between ice algae and under-ice phytoplankton in Saroma-Ko Lagoon, Japan: a comparative taxonomic diatom analysis with microscopy and DNA barcoding Yoshida, K Hattori, H Toyota, T McMinn, A Suzuki, K 2020 https://eprints.utas.edu.au/35311/ unknown Springer-Verlag Yoshida, K orcid:0000-0001-5768-8561 , Hattori, H, Toyota, T, McMinn, A orcid:0000-0002-2133-3854 and Suzuki, K 2020 , 'Differences in diversity and photoprotection capability between ice algae and under-ice phytoplankton in Saroma-Ko Lagoon, Japan: a comparative taxonomic diatom analysis with microscopy and DNA barcoding' , Polar Biology, vol. 43 , 1873–1885 , doi:10.1007/s00300-020-02751-x <http://dx.doi.org/10.1007/s00300-020-02751-x>. sea ice Saroma photosynthesis sea-ice algae phytoplankton microscopy DNA barcoding diversity algal pigments Article PeerReviewed 2020 ftunivtasmania https://doi.org/10.1007/s00300-020-02751-x 2021-10-04T22:19:16Z Sea ice algae, comprised mainly of diatoms, are the main primary producers in polar ecosystems, and they are generally distributed with the highest biomass at the bottom of ice. The taxonomy of ice algae has been traditionally investigated using light microscopy, but molecular techniques, including pigment analysis, have recently provided new insights into the diversity and physiology of ice algae. However, no comparative taxonomic survey has been conducted for ice algae thus far. Here, we investigated differences and similarities in (1) the diversity and (2) the photosynthetic strategies of diatom communities in sea ice and the underlying seawater of Saroma-Ko Lagoon, Hokkaido, Japan, using algal pigment signatures determined by ultra-high performance liquid chromatography, light and scanning electron microscopy and Ion Torrent next-generation sequencing techniques targeting the 18S rRNA gene (i.e., DNA barcoding). Ice algae typically possessed greater biomass (> 20-fold) and chlorophyll (Chl) a breakdown products than under-ice phytoplankton, suggesting that the ice algae formed blooms, and cell senescence and disruption could be significant mitigating factors. At the genus level, the diversity of diatoms in sea ice was higher than in under-ice seawater, although the evenness was comparable or lower in ice algae than in under-ice phytoplankton. Ice algae had a larger xanthophyll pool size and a higher ratio of photoprotective to photosynthetic pigments (11-fold and 4.5-fold higher, respectively) than under-ice phytoplankton. The results indicated that ice algae were well adapted to changes in the light regime, which could partly support their survival capability and high taxonomic diversity. Article in Journal/Newspaper ice algae Polar Biology Sea ice University of Tasmania: UTas ePrints Polar Biology 43 11 1873 1885 |
institution |
Open Polar |
collection |
University of Tasmania: UTas ePrints |
op_collection_id |
ftunivtasmania |
language |
unknown |
topic |
sea ice Saroma photosynthesis sea-ice algae phytoplankton microscopy DNA barcoding diversity algal pigments |
spellingShingle |
sea ice Saroma photosynthesis sea-ice algae phytoplankton microscopy DNA barcoding diversity algal pigments Yoshida, K Hattori, H Toyota, T McMinn, A Suzuki, K Differences in diversity and photoprotection capability between ice algae and under-ice phytoplankton in Saroma-Ko Lagoon, Japan: a comparative taxonomic diatom analysis with microscopy and DNA barcoding |
topic_facet |
sea ice Saroma photosynthesis sea-ice algae phytoplankton microscopy DNA barcoding diversity algal pigments |
description |
Sea ice algae, comprised mainly of diatoms, are the main primary producers in polar ecosystems, and they are generally distributed with the highest biomass at the bottom of ice. The taxonomy of ice algae has been traditionally investigated using light microscopy, but molecular techniques, including pigment analysis, have recently provided new insights into the diversity and physiology of ice algae. However, no comparative taxonomic survey has been conducted for ice algae thus far. Here, we investigated differences and similarities in (1) the diversity and (2) the photosynthetic strategies of diatom communities in sea ice and the underlying seawater of Saroma-Ko Lagoon, Hokkaido, Japan, using algal pigment signatures determined by ultra-high performance liquid chromatography, light and scanning electron microscopy and Ion Torrent next-generation sequencing techniques targeting the 18S rRNA gene (i.e., DNA barcoding). Ice algae typically possessed greater biomass (> 20-fold) and chlorophyll (Chl) a breakdown products than under-ice phytoplankton, suggesting that the ice algae formed blooms, and cell senescence and disruption could be significant mitigating factors. At the genus level, the diversity of diatoms in sea ice was higher than in under-ice seawater, although the evenness was comparable or lower in ice algae than in under-ice phytoplankton. Ice algae had a larger xanthophyll pool size and a higher ratio of photoprotective to photosynthetic pigments (11-fold and 4.5-fold higher, respectively) than under-ice phytoplankton. The results indicated that ice algae were well adapted to changes in the light regime, which could partly support their survival capability and high taxonomic diversity. |
format |
Article in Journal/Newspaper |
author |
Yoshida, K Hattori, H Toyota, T McMinn, A Suzuki, K |
author_facet |
Yoshida, K Hattori, H Toyota, T McMinn, A Suzuki, K |
author_sort |
Yoshida, K |
title |
Differences in diversity and photoprotection capability between ice algae and under-ice phytoplankton in Saroma-Ko Lagoon, Japan: a comparative taxonomic diatom analysis with microscopy and DNA barcoding |
title_short |
Differences in diversity and photoprotection capability between ice algae and under-ice phytoplankton in Saroma-Ko Lagoon, Japan: a comparative taxonomic diatom analysis with microscopy and DNA barcoding |
title_full |
Differences in diversity and photoprotection capability between ice algae and under-ice phytoplankton in Saroma-Ko Lagoon, Japan: a comparative taxonomic diatom analysis with microscopy and DNA barcoding |
title_fullStr |
Differences in diversity and photoprotection capability between ice algae and under-ice phytoplankton in Saroma-Ko Lagoon, Japan: a comparative taxonomic diatom analysis with microscopy and DNA barcoding |
title_full_unstemmed |
Differences in diversity and photoprotection capability between ice algae and under-ice phytoplankton in Saroma-Ko Lagoon, Japan: a comparative taxonomic diatom analysis with microscopy and DNA barcoding |
title_sort |
differences in diversity and photoprotection capability between ice algae and under-ice phytoplankton in saroma-ko lagoon, japan: a comparative taxonomic diatom analysis with microscopy and dna barcoding |
publisher |
Springer-Verlag |
publishDate |
2020 |
url |
https://eprints.utas.edu.au/35311/ |
genre |
ice algae Polar Biology Sea ice |
genre_facet |
ice algae Polar Biology Sea ice |
op_relation |
Yoshida, K orcid:0000-0001-5768-8561 , Hattori, H, Toyota, T, McMinn, A orcid:0000-0002-2133-3854 and Suzuki, K 2020 , 'Differences in diversity and photoprotection capability between ice algae and under-ice phytoplankton in Saroma-Ko Lagoon, Japan: a comparative taxonomic diatom analysis with microscopy and DNA barcoding' , Polar Biology, vol. 43 , 1873–1885 , doi:10.1007/s00300-020-02751-x <http://dx.doi.org/10.1007/s00300-020-02751-x>. |
op_doi |
https://doi.org/10.1007/s00300-020-02751-x |
container_title |
Polar Biology |
container_volume |
43 |
container_issue |
11 |
container_start_page |
1873 |
op_container_end_page |
1885 |
_version_ |
1766026863473328128 |