Insights into the production and role of nitric oxide in the Antarctic sea‐ice diatom Fragilariopsis cylindrus
Nitric oxide (NO) is widely recognized as an important transmitter molecule in biological systems, from animals to plants and microbes. However, the role of NO in marine photosynthetic microbes remains unclear and even less is known about the role of this metabolite in Antarctic sea‐ice diatoms. Usi...
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Blackwell Publishing Inc
2020
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ftunivtasmania:oai:eprints.utas.edu.au:33435 2023-05-15T13:31:54+02:00 Insights into the production and role of nitric oxide in the Antarctic sea‐ice diatom Fragilariopsis cylindrus Kennedy, FC Martin, A McMinn, A 2020 https://eprints.utas.edu.au/33435/ unknown Blackwell Publishing Inc Kennedy, FC orcid:0000-0003-1796-0764 , Martin, A orcid:0000-0001-8260-5529 and McMinn, A orcid:0000-0002-2133-3854 2020 , 'Insights into the production and role of nitric oxide in the Antarctic sea‐ice diatom Fragilariopsis cylindrus' , Journal of Phycology , pp. 1-12 , doi:10.1111/jpy.13027 <http://dx.doi.org/10.1111/jpy.13027>. Antarctic Fragilariopsis cylindrus microelectrodes microfluidics nitric oxide sea-ice Article PeerReviewed 2020 ftunivtasmania https://doi.org/10.1111/jpy.13027 2021-10-04T22:17:56Z Nitric oxide (NO) is widely recognized as an important transmitter molecule in biological systems, from animals to plants and microbes. However, the role of NO in marine photosynthetic microbes remains unclear and even less is known about the role of this metabolite in Antarctic sea‐ice diatoms. Using a combination of microsensors, microfluidic chambers, and artificial sea‐ice tanks, a basic mechanistic insight into NO's dynamics within the Antarctic sea‐ice diatom Fragilariopsis cylindrus was obtained. Results suggest that NO production in F. cylindrus is nitrite‐dependent via nitrate reductase. NO production was abolished upon exposure to light but could be induced in the light when normal photosynthetic electron flow was disrupted. The addition of exogenous NO to cellular suspensions of F. cylindrus negatively influenced growth, disrupted photosynthesis, and altered non‐photochemical dissipation mechanisms. NO production was also observed when cells were exposed to stressful salinity and temperature regimes. These results suggest that during periods of environmental stress, NO could be produced in F. cylindrus as a “stress signa” molecule. Article in Journal/Newspaper Antarc* Antarctic Sea ice University of Tasmania: UTas ePrints Antarctic The Antarctic Journal of Phycology 56 5 1196 1207 |
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Open Polar |
collection |
University of Tasmania: UTas ePrints |
op_collection_id |
ftunivtasmania |
language |
unknown |
topic |
Antarctic Fragilariopsis cylindrus microelectrodes microfluidics nitric oxide sea-ice |
spellingShingle |
Antarctic Fragilariopsis cylindrus microelectrodes microfluidics nitric oxide sea-ice Kennedy, FC Martin, A McMinn, A Insights into the production and role of nitric oxide in the Antarctic sea‐ice diatom Fragilariopsis cylindrus |
topic_facet |
Antarctic Fragilariopsis cylindrus microelectrodes microfluidics nitric oxide sea-ice |
description |
Nitric oxide (NO) is widely recognized as an important transmitter molecule in biological systems, from animals to plants and microbes. However, the role of NO in marine photosynthetic microbes remains unclear and even less is known about the role of this metabolite in Antarctic sea‐ice diatoms. Using a combination of microsensors, microfluidic chambers, and artificial sea‐ice tanks, a basic mechanistic insight into NO's dynamics within the Antarctic sea‐ice diatom Fragilariopsis cylindrus was obtained. Results suggest that NO production in F. cylindrus is nitrite‐dependent via nitrate reductase. NO production was abolished upon exposure to light but could be induced in the light when normal photosynthetic electron flow was disrupted. The addition of exogenous NO to cellular suspensions of F. cylindrus negatively influenced growth, disrupted photosynthesis, and altered non‐photochemical dissipation mechanisms. NO production was also observed when cells were exposed to stressful salinity and temperature regimes. These results suggest that during periods of environmental stress, NO could be produced in F. cylindrus as a “stress signa” molecule. |
format |
Article in Journal/Newspaper |
author |
Kennedy, FC Martin, A McMinn, A |
author_facet |
Kennedy, FC Martin, A McMinn, A |
author_sort |
Kennedy, FC |
title |
Insights into the production and role of nitric oxide in the Antarctic sea‐ice diatom Fragilariopsis cylindrus |
title_short |
Insights into the production and role of nitric oxide in the Antarctic sea‐ice diatom Fragilariopsis cylindrus |
title_full |
Insights into the production and role of nitric oxide in the Antarctic sea‐ice diatom Fragilariopsis cylindrus |
title_fullStr |
Insights into the production and role of nitric oxide in the Antarctic sea‐ice diatom Fragilariopsis cylindrus |
title_full_unstemmed |
Insights into the production and role of nitric oxide in the Antarctic sea‐ice diatom Fragilariopsis cylindrus |
title_sort |
insights into the production and role of nitric oxide in the antarctic sea‐ice diatom fragilariopsis cylindrus |
publisher |
Blackwell Publishing Inc |
publishDate |
2020 |
url |
https://eprints.utas.edu.au/33435/ |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Sea ice |
genre_facet |
Antarc* Antarctic Sea ice |
op_relation |
Kennedy, FC orcid:0000-0003-1796-0764 , Martin, A orcid:0000-0001-8260-5529 and McMinn, A orcid:0000-0002-2133-3854 2020 , 'Insights into the production and role of nitric oxide in the Antarctic sea‐ice diatom Fragilariopsis cylindrus' , Journal of Phycology , pp. 1-12 , doi:10.1111/jpy.13027 <http://dx.doi.org/10.1111/jpy.13027>. |
op_doi |
https://doi.org/10.1111/jpy.13027 |
container_title |
Journal of Phycology |
container_volume |
56 |
container_issue |
5 |
container_start_page |
1196 |
op_container_end_page |
1207 |
_version_ |
1766022243392946176 |