In situ light responses of the proteorhodopsin-bearing Antarctic sea-ice bacterium, Psychroflexus torques
Proteorhodopsin (PR) is a wide-spread protein found in many marine prokaryotes. PR allows for the potential conversion of solar energy to ATP, possibly assisting in cellular growth and survival during periods of high environmental stress. PR utilises either blue or green light through a single amino...
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ftunivtasmania:oai:eprints.utas.edu.au:25201 2023-05-15T13:31:52+02:00 In situ light responses of the proteorhodopsin-bearing Antarctic sea-ice bacterium, Psychroflexus torques Burr, DJ Martin, A Maas, EW Ryan, KG 2017 https://eprints.utas.edu.au/25201/ https://doi.org/10.1038/ismej.2017.65 unknown Nature Publishing Group Burr, DJ, Martin, A orcid:0000-0001-8260-5529 , Maas, EW and Ryan, KG 2017 , 'In situ light responses of the proteorhodopsin-bearing Antarctic sea-ice bacterium, Psychroflexus torques' , ISME Journal, vol. 11 , pp. 2155-2158 , doi:10.1038/ismej.2017.65 <http://dx.doi.org/10.1038/ismej.2017.65>. Antarctica sea ice algae proteorhodopsin Article PeerReviewed 2017 ftunivtasmania https://doi.org/10.1038/ismej.2017.65 2021-09-13T22:16:42Z Proteorhodopsin (PR) is a wide-spread protein found in many marine prokaryotes. PR allows for the potential conversion of solar energy to ATP, possibly assisting in cellular growth and survival during periods of high environmental stress. PR utilises either blue or green light through a single amino acid substitution. We incubated the PR-bearing bacterium Psychroflexus torquis 50 cm deep within Antarctic sea ice for 13 days, exposing cultures to diurnal fluctuations in light and temperature. Enhanced growth occurred most prominently in cultures incubated under irradiance levels of ∼50 μmol photons m−2 s−1, suggesting PR provides a strong selective advantage. In addition, cultures grown under blue light yielded over 5.5 times more live cells per photon compared to green-light incubations. Because P. torquis expresses an apparently ‘green-shifted’ PR gene variant, this finding infers that the spectral tuning of PR is more complex than previously thought. This study supports the theory that PR provides additional energy to bacteria under sub-optimal conditions, and raises several points of interest to be addressed by future research. Article in Journal/Newspaper Antarc* Antarctic Antarctica ice algae Sea ice University of Tasmania: UTas ePrints Antarctic The ISME Journal 11 9 2155 2158 |
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University of Tasmania: UTas ePrints |
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ftunivtasmania |
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topic |
Antarctica sea ice algae proteorhodopsin |
spellingShingle |
Antarctica sea ice algae proteorhodopsin Burr, DJ Martin, A Maas, EW Ryan, KG In situ light responses of the proteorhodopsin-bearing Antarctic sea-ice bacterium, Psychroflexus torques |
topic_facet |
Antarctica sea ice algae proteorhodopsin |
description |
Proteorhodopsin (PR) is a wide-spread protein found in many marine prokaryotes. PR allows for the potential conversion of solar energy to ATP, possibly assisting in cellular growth and survival during periods of high environmental stress. PR utilises either blue or green light through a single amino acid substitution. We incubated the PR-bearing bacterium Psychroflexus torquis 50 cm deep within Antarctic sea ice for 13 days, exposing cultures to diurnal fluctuations in light and temperature. Enhanced growth occurred most prominently in cultures incubated under irradiance levels of ∼50 μmol photons m−2 s−1, suggesting PR provides a strong selective advantage. In addition, cultures grown under blue light yielded over 5.5 times more live cells per photon compared to green-light incubations. Because P. torquis expresses an apparently ‘green-shifted’ PR gene variant, this finding infers that the spectral tuning of PR is more complex than previously thought. This study supports the theory that PR provides additional energy to bacteria under sub-optimal conditions, and raises several points of interest to be addressed by future research. |
format |
Article in Journal/Newspaper |
author |
Burr, DJ Martin, A Maas, EW Ryan, KG |
author_facet |
Burr, DJ Martin, A Maas, EW Ryan, KG |
author_sort |
Burr, DJ |
title |
In situ light responses of the proteorhodopsin-bearing Antarctic sea-ice bacterium, Psychroflexus torques |
title_short |
In situ light responses of the proteorhodopsin-bearing Antarctic sea-ice bacterium, Psychroflexus torques |
title_full |
In situ light responses of the proteorhodopsin-bearing Antarctic sea-ice bacterium, Psychroflexus torques |
title_fullStr |
In situ light responses of the proteorhodopsin-bearing Antarctic sea-ice bacterium, Psychroflexus torques |
title_full_unstemmed |
In situ light responses of the proteorhodopsin-bearing Antarctic sea-ice bacterium, Psychroflexus torques |
title_sort |
in situ light responses of the proteorhodopsin-bearing antarctic sea-ice bacterium, psychroflexus torques |
publisher |
Nature Publishing Group |
publishDate |
2017 |
url |
https://eprints.utas.edu.au/25201/ https://doi.org/10.1038/ismej.2017.65 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica ice algae Sea ice |
genre_facet |
Antarc* Antarctic Antarctica ice algae Sea ice |
op_relation |
Burr, DJ, Martin, A orcid:0000-0001-8260-5529 , Maas, EW and Ryan, KG 2017 , 'In situ light responses of the proteorhodopsin-bearing Antarctic sea-ice bacterium, Psychroflexus torques' , ISME Journal, vol. 11 , pp. 2155-2158 , doi:10.1038/ismej.2017.65 <http://dx.doi.org/10.1038/ismej.2017.65>. |
op_doi |
https://doi.org/10.1038/ismej.2017.65 |
container_title |
The ISME Journal |
container_volume |
11 |
container_issue |
9 |
container_start_page |
2155 |
op_container_end_page |
2158 |
_version_ |
1766021741763624960 |