DNA as a dietary biomarker in Antarctic krill, Euphausia superba
The diet of Antarctic krill (Euphausia superba) has been studied using a variety of techniques, but current methods still suffer from problems that are difficult to solve. This study examined an alternative approach utilizing DNA as a prey biomarker. Methods were developed for the preservation, extr...
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Online Access: | https://eprints.utas.edu.au/2162/ https://eprints.utas.edu.au/2162/1/Passmoreetal2006_DNA.pdf https://doi.org/10.1007/s10126-005-6088-8 |
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ftunivtasmania:oai:eprints.utas.edu.au:2162 2023-05-15T13:36:46+02:00 DNA as a dietary biomarker in Antarctic krill, Euphausia superba Passmore, AJ Jarman, SN Swadling, KM 2006-12 application/pdf https://eprints.utas.edu.au/2162/ https://eprints.utas.edu.au/2162/1/Passmoreetal2006_DNA.pdf https://doi.org/10.1007/s10126-005-6088-8 en eng https://eprints.utas.edu.au/2162/1/Passmoreetal2006_DNA.pdf Passmore, AJ, Jarman, SN and Swadling, KM 2006 , 'DNA as a dietary biomarker in Antarctic krill, Euphausia superba' , Marine Biotechnology, vol. 8, no. 6 , pp. 686-696 , doi:10.1007/s10126-005-6088-8 <http://dx.doi.org/10.1007/s10126-005-6088-8>. cc_utas 270702 Marine and Estuarine Ecology (incl. Marine Ichthyology) diet analysis — foodweb — trophic Article PeerReviewed 2006 ftunivtasmania https://doi.org/10.1007/s10126-005-6088-8 2020-05-30T07:15:54Z The diet of Antarctic krill (Euphausia superba) has been studied using a variety of techniques, but current methods still suffer from problems that are difficult to solve. This study examined an alternative approach utilizing DNA as a prey biomarker. Methods were developed for the preservation, extraction, and identification of prey DNA from krill collected in the field. Group-specific polymerase chain reaction (PCR) was used to amplify diatom prey (Phylum: Bacillariophyta) and the results from DNA clone libraries were compared with microscopic diet analysis. DNA analysis was superior to microscopy for prey detection. However, differences in prey relative abundance estimates between the two techniques suggested some bias in the DNAbased estimates. Quantification showed that large amounts of prey DNA had been successfully preserved and extracted. Overall the results suggest that the application of DNA-based diet analysis to krill warrants further investigation, particularly for prey that are difficult to study using other methods. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Euphausia superba University of Tasmania: UTas ePrints Antarctic Marine Biotechnology 8 6 686 696 |
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Open Polar |
collection |
University of Tasmania: UTas ePrints |
op_collection_id |
ftunivtasmania |
language |
English |
topic |
270702 Marine and Estuarine Ecology (incl. Marine Ichthyology) diet analysis — foodweb — trophic |
spellingShingle |
270702 Marine and Estuarine Ecology (incl. Marine Ichthyology) diet analysis — foodweb — trophic Passmore, AJ Jarman, SN Swadling, KM DNA as a dietary biomarker in Antarctic krill, Euphausia superba |
topic_facet |
270702 Marine and Estuarine Ecology (incl. Marine Ichthyology) diet analysis — foodweb — trophic |
description |
The diet of Antarctic krill (Euphausia superba) has been studied using a variety of techniques, but current methods still suffer from problems that are difficult to solve. This study examined an alternative approach utilizing DNA as a prey biomarker. Methods were developed for the preservation, extraction, and identification of prey DNA from krill collected in the field. Group-specific polymerase chain reaction (PCR) was used to amplify diatom prey (Phylum: Bacillariophyta) and the results from DNA clone libraries were compared with microscopic diet analysis. DNA analysis was superior to microscopy for prey detection. However, differences in prey relative abundance estimates between the two techniques suggested some bias in the DNAbased estimates. Quantification showed that large amounts of prey DNA had been successfully preserved and extracted. Overall the results suggest that the application of DNA-based diet analysis to krill warrants further investigation, particularly for prey that are difficult to study using other methods. |
format |
Article in Journal/Newspaper |
author |
Passmore, AJ Jarman, SN Swadling, KM |
author_facet |
Passmore, AJ Jarman, SN Swadling, KM |
author_sort |
Passmore, AJ |
title |
DNA as a dietary biomarker in Antarctic krill, Euphausia superba |
title_short |
DNA as a dietary biomarker in Antarctic krill, Euphausia superba |
title_full |
DNA as a dietary biomarker in Antarctic krill, Euphausia superba |
title_fullStr |
DNA as a dietary biomarker in Antarctic krill, Euphausia superba |
title_full_unstemmed |
DNA as a dietary biomarker in Antarctic krill, Euphausia superba |
title_sort |
dna as a dietary biomarker in antarctic krill, euphausia superba |
publishDate |
2006 |
url |
https://eprints.utas.edu.au/2162/ https://eprints.utas.edu.au/2162/1/Passmoreetal2006_DNA.pdf https://doi.org/10.1007/s10126-005-6088-8 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba |
op_relation |
https://eprints.utas.edu.au/2162/1/Passmoreetal2006_DNA.pdf Passmore, AJ, Jarman, SN and Swadling, KM 2006 , 'DNA as a dietary biomarker in Antarctic krill, Euphausia superba' , Marine Biotechnology, vol. 8, no. 6 , pp. 686-696 , doi:10.1007/s10126-005-6088-8 <http://dx.doi.org/10.1007/s10126-005-6088-8>. |
op_rights |
cc_utas |
op_doi |
https://doi.org/10.1007/s10126-005-6088-8 |
container_title |
Marine Biotechnology |
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8 |
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6 |
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1766083638156328960 |