Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean
The abundance, spatial distribution and diversity of class Flavobacteria were investigated in the Southern Ocean euphotic zone across a latitudinal transect and in the ice pack off Eastern Antarctica. Surface seawater samples filter-fractionated into 0.8 μm particulate and 0.2 m planktonic fractions...
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ftunivtasmania:oai:eprints.utas.edu.au:2129 2023-05-15T13:36:46+02:00 Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean Abell, GCJ Bowman, JP 2005 application/pdf https://eprints.utas.edu.au/2129/ https://eprints.utas.edu.au/2129/1/Abell_Bowman_2005a.pdf https://doi.org/10.1016/j.femsec.2004.09.001 en eng https://eprints.utas.edu.au/2129/1/Abell_Bowman_2005a.pdf Abell, GCJ and Bowman, JP 2005 , 'Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean' , FEMS Microbiology Ecology, vol. 51 , pp. 265-277 , doi:10.1016/j.femsec.2004.09.001 <http://dx.doi.org/10.1016/j.femsec.2004.09.001>. cc_utas 270307 Microbial Ecology Southern Ocean DGGE Flavobacteria Cytophaga–Flavobacterium–Bacteroides group Biogeography Psychrophilic bacteria Article PeerReviewed 2005 ftunivtasmania https://doi.org/10.1016/j.femsec.2004.09.001 2020-05-30T07:15:47Z The abundance, spatial distribution and diversity of class Flavobacteria were investigated in the Southern Ocean euphotic zone across a latitudinal transect and in the ice pack off Eastern Antarctica. Surface seawater samples filter-fractionated into 0.8 μm particulate and 0.2 m planktonic fractions were investigated with different molecular techniques. The abundance of particle-associated Flavobacteria, ascertained with real-time PCR and DGGE band analysis using Flavobacteria-specific primers, was found to be significantly higher in Polar Front Zone (PFZ) and Antarctic Zone (AZ) water samples than in nutrient limited Temperate Zone (TZ) and Sub-Antarctic Zone (SAZ) waters. Abundance of particle-associated Flavobacteria correlated positively with seawater chlorophyll a and nutrient concentrations, suggesting that increased Flavobacteria abundance may relate to enhanced primary production in the PFZ and AZ. This is supported by comparison of DGGE profiles that demonstrated significant differences in the total Flavobacteria community structure and 16S rRNA gene diversity between samples from the PFZ and AZ and those from TZ and SAZ. Sequence analysis revealed a broad diversity amongst class Flavobacteria in the Southern Ocean with several Flavobacteria clades detected in PFZ and AZ waters not detected in TZ and SAZ waters that putatively represent psychrophilic taxa. Sequence data included a large, so far uncultivated, cosmopolitan phylogenetic clade ("DE cluster 2") that is distributed throughout the Southern Ocean. Article in Journal/Newspaper Antarc* Antarctic Antarctica Southern Ocean University of Tasmania: UTas ePrints Antarctic Southern Ocean FEMS Microbiology Ecology 51 2 265 277 |
institution |
Open Polar |
collection |
University of Tasmania: UTas ePrints |
op_collection_id |
ftunivtasmania |
language |
English |
topic |
270307 Microbial Ecology Southern Ocean DGGE Flavobacteria Cytophaga–Flavobacterium–Bacteroides group Biogeography Psychrophilic bacteria |
spellingShingle |
270307 Microbial Ecology Southern Ocean DGGE Flavobacteria Cytophaga–Flavobacterium–Bacteroides group Biogeography Psychrophilic bacteria Abell, GCJ Bowman, JP Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean |
topic_facet |
270307 Microbial Ecology Southern Ocean DGGE Flavobacteria Cytophaga–Flavobacterium–Bacteroides group Biogeography Psychrophilic bacteria |
description |
The abundance, spatial distribution and diversity of class Flavobacteria were investigated in the Southern Ocean euphotic zone across a latitudinal transect and in the ice pack off Eastern Antarctica. Surface seawater samples filter-fractionated into 0.8 μm particulate and 0.2 m planktonic fractions were investigated with different molecular techniques. The abundance of particle-associated Flavobacteria, ascertained with real-time PCR and DGGE band analysis using Flavobacteria-specific primers, was found to be significantly higher in Polar Front Zone (PFZ) and Antarctic Zone (AZ) water samples than in nutrient limited Temperate Zone (TZ) and Sub-Antarctic Zone (SAZ) waters. Abundance of particle-associated Flavobacteria correlated positively with seawater chlorophyll a and nutrient concentrations, suggesting that increased Flavobacteria abundance may relate to enhanced primary production in the PFZ and AZ. This is supported by comparison of DGGE profiles that demonstrated significant differences in the total Flavobacteria community structure and 16S rRNA gene diversity between samples from the PFZ and AZ and those from TZ and SAZ. Sequence analysis revealed a broad diversity amongst class Flavobacteria in the Southern Ocean with several Flavobacteria clades detected in PFZ and AZ waters not detected in TZ and SAZ waters that putatively represent psychrophilic taxa. Sequence data included a large, so far uncultivated, cosmopolitan phylogenetic clade ("DE cluster 2") that is distributed throughout the Southern Ocean. |
format |
Article in Journal/Newspaper |
author |
Abell, GCJ Bowman, JP |
author_facet |
Abell, GCJ Bowman, JP |
author_sort |
Abell, GCJ |
title |
Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean |
title_short |
Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean |
title_full |
Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean |
title_fullStr |
Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean |
title_full_unstemmed |
Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean |
title_sort |
ecological and biogeographic relationships of class flavobacteria in the southern ocean |
publishDate |
2005 |
url |
https://eprints.utas.edu.au/2129/ https://eprints.utas.edu.au/2129/1/Abell_Bowman_2005a.pdf https://doi.org/10.1016/j.femsec.2004.09.001 |
geographic |
Antarctic Southern Ocean |
geographic_facet |
Antarctic Southern Ocean |
genre |
Antarc* Antarctic Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica Southern Ocean |
op_relation |
https://eprints.utas.edu.au/2129/1/Abell_Bowman_2005a.pdf Abell, GCJ and Bowman, JP 2005 , 'Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean' , FEMS Microbiology Ecology, vol. 51 , pp. 265-277 , doi:10.1016/j.femsec.2004.09.001 <http://dx.doi.org/10.1016/j.femsec.2004.09.001>. |
op_rights |
cc_utas |
op_doi |
https://doi.org/10.1016/j.femsec.2004.09.001 |
container_title |
FEMS Microbiology Ecology |
container_volume |
51 |
container_issue |
2 |
container_start_page |
265 |
op_container_end_page |
277 |
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1766083633964122112 |