Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture
The sea ice microbial community plays a key role in the productivity of the Southern Ocean. Exopolysaccharide (EPS) is a major component of the exopolymer secreted by many marine bacteria to enhance survival and is abundant in sea ice brine channels, but little is known about its function there. Thi...
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ftunivtasmania:oai:eprints.utas.edu.au:2108 2023-05-15T13:36:46+02:00 Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture Nichols, CAM Bowman, JP Guezennec, J 2005 application/pdf https://eprints.utas.edu.au/2108/ https://eprints.utas.edu.au/2108/1/Nichols_etal_2005.pdf https://doi.org/10.1128/AEM.71.7.3519–3523.2005 en eng https://eprints.utas.edu.au/2108/1/Nichols_etal_2005.pdf Nichols, CAM, Bowman, JP and Guezennec, J 2005 , 'Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture' , Applied and Environmental Microbiology, vol. 71, no. 7 , pp. 3519-3523 , doi:10.1128/AEM.71.7.3519–3523.2005 <http://dx.doi.org/10.1128/AEM.71.7.3519–3523.2005>. cc_utas 270307 Microbial Ecology Article PeerReviewed 2005 ftunivtasmania https://doi.org/10.1128/AEM.71.7.3519–3523.2005 2020-05-30T07:15:47Z The sea ice microbial community plays a key role in the productivity of the Southern Ocean. Exopolysaccharide (EPS) is a major component of the exopolymer secreted by many marine bacteria to enhance survival and is abundant in sea ice brine channels, but little is known about its function there. This study investigated the effects of temperature on EPS production in batch culture by CAM025, a marine bacterium isolated from sea ice sampled from the Southern Ocean. Previous studies have shown that CAM025 is a member of the genus Pseudoalteromonas and therefore belongs to a group found to be abundant in sea ice by culture-dependent and -independent techniques. Batch cultures were grown at 2°C, 10°C, and 20°C, and cell number, optical density, pH, glucose concentration, and viscosity were monitored. The yield of EPS at 2°C and 10°C was 30 times higher than at 20°C, which is the optimum growth temperature for many psychrotolerant strains. EPS may have a cryoprotective role in brine channels of sea ice, where extremes of high salinity and low temperature impose pressures on microbial growth and survival. The EPS produced at 2°C and 10°C had a higher uronic acid content than that produced at 20°C. The availability of iron as a trace metal is of critical importance in the Southern Ocean, where it is known to limit primary production. EPS from strain CAM025 is polyanionic and may bind dissolved cations such at trace metals, and therefore the presence of bacterial EPS in the Antarctic marine environment may have important ecological implications. Article in Journal/Newspaper Antarc* Antarctic Sea ice Southern Ocean University of Tasmania: UTas ePrints Antarctic Southern Ocean The Antarctic Applied and Environmental Microbiology 71 7 3519 3523 |
institution |
Open Polar |
collection |
University of Tasmania: UTas ePrints |
op_collection_id |
ftunivtasmania |
language |
English |
topic |
270307 Microbial Ecology |
spellingShingle |
270307 Microbial Ecology Nichols, CAM Bowman, JP Guezennec, J Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture |
topic_facet |
270307 Microbial Ecology |
description |
The sea ice microbial community plays a key role in the productivity of the Southern Ocean. Exopolysaccharide (EPS) is a major component of the exopolymer secreted by many marine bacteria to enhance survival and is abundant in sea ice brine channels, but little is known about its function there. This study investigated the effects of temperature on EPS production in batch culture by CAM025, a marine bacterium isolated from sea ice sampled from the Southern Ocean. Previous studies have shown that CAM025 is a member of the genus Pseudoalteromonas and therefore belongs to a group found to be abundant in sea ice by culture-dependent and -independent techniques. Batch cultures were grown at 2°C, 10°C, and 20°C, and cell number, optical density, pH, glucose concentration, and viscosity were monitored. The yield of EPS at 2°C and 10°C was 30 times higher than at 20°C, which is the optimum growth temperature for many psychrotolerant strains. EPS may have a cryoprotective role in brine channels of sea ice, where extremes of high salinity and low temperature impose pressures on microbial growth and survival. The EPS produced at 2°C and 10°C had a higher uronic acid content than that produced at 20°C. The availability of iron as a trace metal is of critical importance in the Southern Ocean, where it is known to limit primary production. EPS from strain CAM025 is polyanionic and may bind dissolved cations such at trace metals, and therefore the presence of bacterial EPS in the Antarctic marine environment may have important ecological implications. |
format |
Article in Journal/Newspaper |
author |
Nichols, CAM Bowman, JP Guezennec, J |
author_facet |
Nichols, CAM Bowman, JP Guezennec, J |
author_sort |
Nichols, CAM |
title |
Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture |
title_short |
Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture |
title_full |
Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture |
title_fullStr |
Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture |
title_full_unstemmed |
Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture |
title_sort |
effects of incubation temperature on growth and production of exopolysaccharides by an antarctic sea ice bacterium grown in batch culture |
publishDate |
2005 |
url |
https://eprints.utas.edu.au/2108/ https://eprints.utas.edu.au/2108/1/Nichols_etal_2005.pdf https://doi.org/10.1128/AEM.71.7.3519–3523.2005 |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic Sea ice Southern Ocean |
op_relation |
https://eprints.utas.edu.au/2108/1/Nichols_etal_2005.pdf Nichols, CAM, Bowman, JP and Guezennec, J 2005 , 'Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture' , Applied and Environmental Microbiology, vol. 71, no. 7 , pp. 3519-3523 , doi:10.1128/AEM.71.7.3519–3523.2005 <http://dx.doi.org/10.1128/AEM.71.7.3519–3523.2005>. |
op_rights |
cc_utas |
op_doi |
https://doi.org/10.1128/AEM.71.7.3519–3523.2005 |
container_title |
Applied and Environmental Microbiology |
container_volume |
71 |
container_issue |
7 |
container_start_page |
3519 |
op_container_end_page |
3523 |
_version_ |
1766083630109556736 |