Chlorophyll fluorescence imaging analysis of the responses of Antarctic bottom-ice algae to light and salinity during melting
Bottom-ice algae within Antarctic sea ice were examined using chlorophyll fluorescence imaging. The detailed structure of the bottom-ice algal community growing in the platelet and congelation layers of solid pieces of sea ice was evident for the first time in chlorophyll imaging mode. Strands of fl...
Published in: | Journal of Experimental Marine Biology and Ecology |
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Online Access: | https://eprints.utas.edu.au/12121/ https://eprints.utas.edu.au/12121/2/Ryan_UV1.pdf https://doi.org/10.1016/j.jembe.2011.01.006 |
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ftunivtasmania:oai:eprints.utas.edu.au:12121 2023-05-15T13:36:47+02:00 Chlorophyll fluorescence imaging analysis of the responses of Antarctic bottom-ice algae to light and salinity during melting Ryan, KG Tay, ML Martin, A McMinn, A Davy, SK 2011 application/pdf https://eprints.utas.edu.au/12121/ https://eprints.utas.edu.au/12121/2/Ryan_UV1.pdf https://doi.org/10.1016/j.jembe.2011.01.006 en eng https://eprints.utas.edu.au/12121/2/Ryan_UV1.pdf Ryan, KG, Tay, ML, Martin, A, McMinn, A and Davy, SK 2011 , 'Chlorophyll fluorescence imaging analysis of the responses of Antarctic bottom-ice algae to light and salinity during melting' , Journal of Experimental Marine Biology and Ecology, vol. 399 , pp. 156-161 , doi:10.1016/j.jembe.2011.01.006 <http://dx.doi.org/10.1016/j.jembe.2011.01.006>. cc_utas Algae Antarctic Chlorophyll fluorescence Imaging PAM Salinity Sea ice Article PeerReviewed 2011 ftunivtasmania https://doi.org/10.1016/j.jembe.2011.01.006 2020-05-30T07:25:53Z Bottom-ice algae within Antarctic sea ice were examined using chlorophyll fluorescence imaging. The detailed structure of the bottom-ice algal community growing in the platelet and congelation layers of solid pieces of sea ice was evident for the first time in chlorophyll imaging mode. Strands of fluorescence representing algal cells were clearly visible growing upward into brine channels in a fine network. Images of effective quantum yield (ФPSII) revealed that theФPSII of algae embedded in the sea ice was approximately 0.5. Furthermore,ФPSII decreased slightly with distance from the ice–water interface. The response of Antarctic sea ice algae to changes in irradiance and salinity, and the effects of slowly warming and melting the ice block sample were examined using this system. The ФPSII of bottom-ice algae decreased as irradiance increased and salinities decreased. Bottom-ice algae appear to be most vulnerable to changes in their environment during the melting process of the ice, and this suggests that algae from this region of the ice may not be able to cope with the stress of melting during summer. Chlorophyll fluorescence imaging provides unprecedented imagery of chlorophyll distribution in sea ice and allows measurement of the responses of sea ice algae to environmental stresses with minimal disruption to their physical habitat. The results obtained with this method are comparable to those obtained with algae that have been melted into liquid culture and this indicates that previous melting protocols reveal meaningful data. In this chlorophyll imaging study, rapid light curves did not saturate and this may prevent further use of this configuration. Article in Journal/Newspaper Antarc* Antarctic ice algae Sea ice University of Tasmania: UTas ePrints Antarctic Journal of Experimental Marine Biology and Ecology 399 2 156 161 |
institution |
Open Polar |
collection |
University of Tasmania: UTas ePrints |
op_collection_id |
ftunivtasmania |
language |
English |
topic |
Algae Antarctic Chlorophyll fluorescence Imaging PAM Salinity Sea ice |
spellingShingle |
Algae Antarctic Chlorophyll fluorescence Imaging PAM Salinity Sea ice Ryan, KG Tay, ML Martin, A McMinn, A Davy, SK Chlorophyll fluorescence imaging analysis of the responses of Antarctic bottom-ice algae to light and salinity during melting |
topic_facet |
Algae Antarctic Chlorophyll fluorescence Imaging PAM Salinity Sea ice |
description |
Bottom-ice algae within Antarctic sea ice were examined using chlorophyll fluorescence imaging. The detailed structure of the bottom-ice algal community growing in the platelet and congelation layers of solid pieces of sea ice was evident for the first time in chlorophyll imaging mode. Strands of fluorescence representing algal cells were clearly visible growing upward into brine channels in a fine network. Images of effective quantum yield (ФPSII) revealed that theФPSII of algae embedded in the sea ice was approximately 0.5. Furthermore,ФPSII decreased slightly with distance from the ice–water interface. The response of Antarctic sea ice algae to changes in irradiance and salinity, and the effects of slowly warming and melting the ice block sample were examined using this system. The ФPSII of bottom-ice algae decreased as irradiance increased and salinities decreased. Bottom-ice algae appear to be most vulnerable to changes in their environment during the melting process of the ice, and this suggests that algae from this region of the ice may not be able to cope with the stress of melting during summer. Chlorophyll fluorescence imaging provides unprecedented imagery of chlorophyll distribution in sea ice and allows measurement of the responses of sea ice algae to environmental stresses with minimal disruption to their physical habitat. The results obtained with this method are comparable to those obtained with algae that have been melted into liquid culture and this indicates that previous melting protocols reveal meaningful data. In this chlorophyll imaging study, rapid light curves did not saturate and this may prevent further use of this configuration. |
format |
Article in Journal/Newspaper |
author |
Ryan, KG Tay, ML Martin, A McMinn, A Davy, SK |
author_facet |
Ryan, KG Tay, ML Martin, A McMinn, A Davy, SK |
author_sort |
Ryan, KG |
title |
Chlorophyll fluorescence imaging analysis of the responses of Antarctic bottom-ice algae to light and salinity during melting |
title_short |
Chlorophyll fluorescence imaging analysis of the responses of Antarctic bottom-ice algae to light and salinity during melting |
title_full |
Chlorophyll fluorescence imaging analysis of the responses of Antarctic bottom-ice algae to light and salinity during melting |
title_fullStr |
Chlorophyll fluorescence imaging analysis of the responses of Antarctic bottom-ice algae to light and salinity during melting |
title_full_unstemmed |
Chlorophyll fluorescence imaging analysis of the responses of Antarctic bottom-ice algae to light and salinity during melting |
title_sort |
chlorophyll fluorescence imaging analysis of the responses of antarctic bottom-ice algae to light and salinity during melting |
publishDate |
2011 |
url |
https://eprints.utas.edu.au/12121/ https://eprints.utas.edu.au/12121/2/Ryan_UV1.pdf https://doi.org/10.1016/j.jembe.2011.01.006 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic ice algae Sea ice |
genre_facet |
Antarc* Antarctic ice algae Sea ice |
op_relation |
https://eprints.utas.edu.au/12121/2/Ryan_UV1.pdf Ryan, KG, Tay, ML, Martin, A, McMinn, A and Davy, SK 2011 , 'Chlorophyll fluorescence imaging analysis of the responses of Antarctic bottom-ice algae to light and salinity during melting' , Journal of Experimental Marine Biology and Ecology, vol. 399 , pp. 156-161 , doi:10.1016/j.jembe.2011.01.006 <http://dx.doi.org/10.1016/j.jembe.2011.01.006>. |
op_rights |
cc_utas |
op_doi |
https://doi.org/10.1016/j.jembe.2011.01.006 |
container_title |
Journal of Experimental Marine Biology and Ecology |
container_volume |
399 |
container_issue |
2 |
container_start_page |
156 |
op_container_end_page |
161 |
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1766084023645372416 |