Recrystallization in a freezing tolerant Antarctic nematode, Panagrolaimus davidi, and an alpine weta, Hemideina maori (Orthoptera; Stenopelmatidae)

• Published in: Cryobiology
• Main Authors: Ramlov, H, Wharton, DA, Wilson, PW
• Format: Article in Journal/Newspaper
• Language: English
• Published: Academic Press Inc Elsevier Science 1996
• Subjects: Biological Sciences; Biochemistry and Cell Biology; Biochemistry and Cell Biology not elsewhere classified; Antarctic; Ringer; Antarc*
• Online Access: https://doi.org/10.1006/cryo.1996.0064; http://www.ncbi.nlm.nih.gov/pubmed/8975688; http://ecite.utas.edu.au/99883

The ability of haemolymph from the freezing tolerant weta, Hemideina maori, and supernatant from homogenates of the freezing tolerant nematode Panagrolaimus davidi to inhibit the recrystallization of ice was examined using the "splat freezing" technique and annealing on a cryomicroscope stage. There was no recrystallization inhibition in weta haemolymph or in insect ringer controls. Recrystallization inhibition was present in the nematode supernatant but this was destroyed by heating and was absent in controls. P. davidi survives intracellular freezing and recrystallization inhibition may be important for the survival of this stress.