Assignment of parentage in triploid species using microsatellite markers with null alleles, an example from Pacific oysters ( Crassostrea gigas )
Triploid production in aquaculture is increasing because of their more profitable growth and reproduction traits. Triploids are mostly produced through mass spawning techniques, meaning that exact pedigree is unknown. The ability to trace the pedigree of high performing triploids would allow selecti...
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2014
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Online Access: | https://doi.org/10.1111/are.12588 http://ecite.utas.edu.au/95891 |
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ftunivtasecite:oai:ecite.utas.edu.au:95891 2023-05-15T15:58:39+02:00 Assignment of parentage in triploid species using microsatellite markers with null alleles, an example from Pacific oysters ( Crassostrea gigas ) Miller, PA Elliott, NG Vaillancourt, RE Koutoulis, A Henshall, JM 2014 https://doi.org/10.1111/are.12588 http://ecite.utas.edu.au/95891 en eng Blackwell Publishing Ltd http://dx.doi.org/10.1111/are.12588 Miller, PA and Elliott, NG and Vaillancourt, RE and Koutoulis, A and Henshall, JM, Assignment of parentage in triploid species using microsatellite markers with null alleles, an example from Pacific oysters ( Crassostrea gigas ), Aquaculture Research, 47, (4) pp. 1288-1298. ISSN 1355-557X (2014) [Refereed Article] http://ecite.utas.edu.au/95891 Biological Sciences Zoology Animal Cell and Molecular Biology Refereed Article PeerReviewed 2014 ftunivtasecite https://doi.org/10.1111/are.12588 2019-12-13T21:58:17Z Triploid production in aquaculture is increasing because of their more profitable growth and reproduction traits. Triploids are mostly produced through mass spawning techniques, meaning that exact pedigree is unknown. The ability to trace the pedigree of high performing triploids would allow selection of broodstock to perpetuate triploids of greater economic value. This study aimed to develop a method of determining parental assignment in triploids and test its accuracy on triploid oysters. Using a likelihood approach and accounting for null allele frequencies, a method was developed which proved to be efficient at determining the pedigree of triploid oysters. This method was able to provide accurate pedigree on simulated data and two commercial cohorts of triploid oysters. The analysis of the triploid cohorts showed that mass spawning to produce triploid oysters, like that for diploid and tetraploids, results in a strong bias in parental contributions, with the effective population size being 34-49% lower than the census population. This highlights the need for pedigree control in breeding programs and indicates that the ability to determine parentage of triploids will be a valuable tool for breeding programs. Article in Journal/Newspaper Crassostrea gigas eCite UTAS (University of Tasmania) Pacific Aquaculture Research 47 4 1288 1298 |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
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English |
topic |
Biological Sciences Zoology Animal Cell and Molecular Biology |
spellingShingle |
Biological Sciences Zoology Animal Cell and Molecular Biology Miller, PA Elliott, NG Vaillancourt, RE Koutoulis, A Henshall, JM Assignment of parentage in triploid species using microsatellite markers with null alleles, an example from Pacific oysters ( Crassostrea gigas ) |
topic_facet |
Biological Sciences Zoology Animal Cell and Molecular Biology |
description |
Triploid production in aquaculture is increasing because of their more profitable growth and reproduction traits. Triploids are mostly produced through mass spawning techniques, meaning that exact pedigree is unknown. The ability to trace the pedigree of high performing triploids would allow selection of broodstock to perpetuate triploids of greater economic value. This study aimed to develop a method of determining parental assignment in triploids and test its accuracy on triploid oysters. Using a likelihood approach and accounting for null allele frequencies, a method was developed which proved to be efficient at determining the pedigree of triploid oysters. This method was able to provide accurate pedigree on simulated data and two commercial cohorts of triploid oysters. The analysis of the triploid cohorts showed that mass spawning to produce triploid oysters, like that for diploid and tetraploids, results in a strong bias in parental contributions, with the effective population size being 34-49% lower than the census population. This highlights the need for pedigree control in breeding programs and indicates that the ability to determine parentage of triploids will be a valuable tool for breeding programs. |
format |
Article in Journal/Newspaper |
author |
Miller, PA Elliott, NG Vaillancourt, RE Koutoulis, A Henshall, JM |
author_facet |
Miller, PA Elliott, NG Vaillancourt, RE Koutoulis, A Henshall, JM |
author_sort |
Miller, PA |
title |
Assignment of parentage in triploid species using microsatellite markers with null alleles, an example from Pacific oysters ( Crassostrea gigas ) |
title_short |
Assignment of parentage in triploid species using microsatellite markers with null alleles, an example from Pacific oysters ( Crassostrea gigas ) |
title_full |
Assignment of parentage in triploid species using microsatellite markers with null alleles, an example from Pacific oysters ( Crassostrea gigas ) |
title_fullStr |
Assignment of parentage in triploid species using microsatellite markers with null alleles, an example from Pacific oysters ( Crassostrea gigas ) |
title_full_unstemmed |
Assignment of parentage in triploid species using microsatellite markers with null alleles, an example from Pacific oysters ( Crassostrea gigas ) |
title_sort |
assignment of parentage in triploid species using microsatellite markers with null alleles, an example from pacific oysters ( crassostrea gigas ) |
publisher |
Blackwell Publishing Ltd |
publishDate |
2014 |
url |
https://doi.org/10.1111/are.12588 http://ecite.utas.edu.au/95891 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
http://dx.doi.org/10.1111/are.12588 Miller, PA and Elliott, NG and Vaillancourt, RE and Koutoulis, A and Henshall, JM, Assignment of parentage in triploid species using microsatellite markers with null alleles, an example from Pacific oysters ( Crassostrea gigas ), Aquaculture Research, 47, (4) pp. 1288-1298. ISSN 1355-557X (2014) [Refereed Article] http://ecite.utas.edu.au/95891 |
op_doi |
https://doi.org/10.1111/are.12588 |
container_title |
Aquaculture Research |
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47 |
container_issue |
4 |
container_start_page |
1288 |
op_container_end_page |
1298 |
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1766394426284834816 |