A method for the use of the radiotracer 55 Fe for microautoradiography and CARD-FISH of natural bacterial communities
Heterotrophic bacteria are key players in the biogeochemical cycle of iron (Fe) in the ocean, but the capability of different bacterial groups to access this micronutrient is ignored thus far. The aim of our study was to develop a protocol for the combined application of microautoradiography (MICRO)...
Published in: | FEMS Microbiology Letters |
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Online Access: | https://doi.org/10.1111/1574-6968.12022 http://www.ncbi.nlm.nih.gov/pubmed/23050807 http://ecite.utas.edu.au/91585 |
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ftunivtasecite:oai:ecite.utas.edu.au:91585 2023-05-15T18:25:35+02:00 A method for the use of the radiotracer 55 Fe for microautoradiography and CARD-FISH of natural bacterial communities Fourquez, MAC Obernosterer, I Blain, S 2012 https://doi.org/10.1111/1574-6968.12022 http://www.ncbi.nlm.nih.gov/pubmed/23050807 http://ecite.utas.edu.au/91585 en eng Blackwell http://dx.doi.org/10.1111/1574-6968.12022 Fourquez, MAC and Obernosterer, I and Blain, S, A method for the use of the radiotracer 55 Fe for microautoradiography and CARD-FISH of natural bacterial communities, F E M S Microbiology Letters: (Federation of European Microbiological Societies), 337, (2) pp. 132-139. ISSN 0378-1097 (2012) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/23050807 http://ecite.utas.edu.au/91585 Earth Sciences Oceanography Biological Oceanography Refereed Article PeerReviewed 2012 ftunivtasecite https://doi.org/10.1111/1574-6968.12022 2019-12-13T21:54:45Z Heterotrophic bacteria are key players in the biogeochemical cycle of iron (Fe) in the ocean, but the capability of different bacterial groups to access this micronutrient is ignored thus far. The aim of our study was to develop a protocol for the combined application of microautoradiography (MICRO) and catalyzed reporter depositionfluorescence in situ hybridization (CARD-FISH) using the radioisotope 55 Fe. Among the different washing solutions tested, Ti-citrate-EDTA was the most efficient for the removal of extracellular 55 Fe providing sufficiently low background values. We further demonstrate that the washing of cells with Ti-citrate-EDTA and the fixation with paraformaldehyde or formaldehyde do not induce leakage of intracellular 55 Fe. Incubating natural bacterial communities collected from contrasting environments, the NW Mediterranean Sea and the Southern Ocean, with 55 Fe revealed that 329% of bacterial cells were associated with silver grains. Combining microautoradiography with CARD-FISH, we demonstrate that the contribution of different bacterial groups to total 55 Fe-incorporating cells was overall reflected by their relative contribution to abundance. An exception to this pattern was the proportionally higher contribution of Gammaproteobacteria , SAR86 and Alteromonas . Our study demonstrates the feasibility of MICRO-CARD-FISH using the radiotracer 55 Fe and provides the first description of marine bacterial assemblages actively incorporating Fe. Article in Journal/Newspaper Southern Ocean eCite UTAS (University of Tasmania) Southern Ocean FEMS Microbiology Letters 337 2 132 139 |
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Open Polar |
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eCite UTAS (University of Tasmania) |
op_collection_id |
ftunivtasecite |
language |
English |
topic |
Earth Sciences Oceanography Biological Oceanography |
spellingShingle |
Earth Sciences Oceanography Biological Oceanography Fourquez, MAC Obernosterer, I Blain, S A method for the use of the radiotracer 55 Fe for microautoradiography and CARD-FISH of natural bacterial communities |
topic_facet |
Earth Sciences Oceanography Biological Oceanography |
description |
Heterotrophic bacteria are key players in the biogeochemical cycle of iron (Fe) in the ocean, but the capability of different bacterial groups to access this micronutrient is ignored thus far. The aim of our study was to develop a protocol for the combined application of microautoradiography (MICRO) and catalyzed reporter depositionfluorescence in situ hybridization (CARD-FISH) using the radioisotope 55 Fe. Among the different washing solutions tested, Ti-citrate-EDTA was the most efficient for the removal of extracellular 55 Fe providing sufficiently low background values. We further demonstrate that the washing of cells with Ti-citrate-EDTA and the fixation with paraformaldehyde or formaldehyde do not induce leakage of intracellular 55 Fe. Incubating natural bacterial communities collected from contrasting environments, the NW Mediterranean Sea and the Southern Ocean, with 55 Fe revealed that 329% of bacterial cells were associated with silver grains. Combining microautoradiography with CARD-FISH, we demonstrate that the contribution of different bacterial groups to total 55 Fe-incorporating cells was overall reflected by their relative contribution to abundance. An exception to this pattern was the proportionally higher contribution of Gammaproteobacteria , SAR86 and Alteromonas . Our study demonstrates the feasibility of MICRO-CARD-FISH using the radiotracer 55 Fe and provides the first description of marine bacterial assemblages actively incorporating Fe. |
format |
Article in Journal/Newspaper |
author |
Fourquez, MAC Obernosterer, I Blain, S |
author_facet |
Fourquez, MAC Obernosterer, I Blain, S |
author_sort |
Fourquez, MAC |
title |
A method for the use of the radiotracer 55 Fe for microautoradiography and CARD-FISH of natural bacterial communities |
title_short |
A method for the use of the radiotracer 55 Fe for microautoradiography and CARD-FISH of natural bacterial communities |
title_full |
A method for the use of the radiotracer 55 Fe for microautoradiography and CARD-FISH of natural bacterial communities |
title_fullStr |
A method for the use of the radiotracer 55 Fe for microautoradiography and CARD-FISH of natural bacterial communities |
title_full_unstemmed |
A method for the use of the radiotracer 55 Fe for microautoradiography and CARD-FISH of natural bacterial communities |
title_sort |
method for the use of the radiotracer 55 fe for microautoradiography and card-fish of natural bacterial communities |
publisher |
Blackwell |
publishDate |
2012 |
url |
https://doi.org/10.1111/1574-6968.12022 http://www.ncbi.nlm.nih.gov/pubmed/23050807 http://ecite.utas.edu.au/91585 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_relation |
http://dx.doi.org/10.1111/1574-6968.12022 Fourquez, MAC and Obernosterer, I and Blain, S, A method for the use of the radiotracer 55 Fe for microautoradiography and CARD-FISH of natural bacterial communities, F E M S Microbiology Letters: (Federation of European Microbiological Societies), 337, (2) pp. 132-139. ISSN 0378-1097 (2012) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/23050807 http://ecite.utas.edu.au/91585 |
op_doi |
https://doi.org/10.1111/1574-6968.12022 |
container_title |
FEMS Microbiology Letters |
container_volume |
337 |
container_issue |
2 |
container_start_page |
132 |
op_container_end_page |
139 |
_version_ |
1766207147193925632 |