An automated pH-controlled culture system for laboratory-based ocean acidification experiments
Using a spectrophotometric pH measurement system, an automated 12-tank culture system was developed,which is capable of maintaining pH levels between 7.51 and 8.00 (local, in-situ pH) within 0.02 pH units at15.1C. The precise control of pH in the individual culture tanks was achieved through the add...
Published in: | Limnology and Oceanography: Methods |
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Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Amer Soc Limnology Oceanography
2010
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Subjects: | |
Online Access: | http://www.aslo.org/ https://doi.org/10.4319/lom.2010.8.686 http://ecite.utas.edu.au/91477 |
Summary: | Using a spectrophotometric pH measurement system, an automated 12-tank culture system was developed,which is capable of maintaining pH levels between 7.51 and 8.00 (local, in-situ pH) within 0.02 pH units at15.1C. The precise control of pH in the individual culture tanks was achieved through the addition of CO2-enriched seawater to unamended seawater. A feed-back system, which automatically adjusted the mixing ratioof unamended and CO2-enriched seawater, ensured that the pH in each tank was kept within 0.02 pH units oftarget values. After each tank adjustment, the spectrophotometric pH measurement was combined with a dissolvedinorganic carbon measurement to automatically re-calculate carbonate chemistry in each tank. The systemwas used to study the growth of the geniculate coralline alga Arthrocardia corymbosa at pH levels of 7.52,7.76, and 7.98. |
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