Preliminary evidence of transcriptional immunomodulation by praziquantel in bluefin tuna and Atlantic salmon in vitro cultures
Praziquantel (PZQ), long-used in veterinary and human medicine for the treatment of helminth parasites, is known to enhance humoral and cellular immune responsiveness in mammals but has unknown direct immunomodulatory capabilities in fish. In the present study, we examined the ability of PZQ to indu...
Published in: | Fish & Shellfish Immunology |
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Academic Press Ltd Elsevier Science Ltd
2014
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Online Access: | https://doi.org/10.1016/j.fsi.2014.02.023 http://www.ncbi.nlm.nih.gov/pubmed/24632044 http://ecite.utas.edu.au/91204 |
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ftunivtasecite:oai:ecite.utas.edu.au:91204 2023-05-15T15:30:45+02:00 Preliminary evidence of transcriptional immunomodulation by praziquantel in bluefin tuna and Atlantic salmon in vitro cultures Polinski, M Bridle, A Neumann, L Nowak, B 2014 https://doi.org/10.1016/j.fsi.2014.02.023 http://www.ncbi.nlm.nih.gov/pubmed/24632044 http://ecite.utas.edu.au/91204 en eng Academic Press Ltd Elsevier Science Ltd http://dx.doi.org/10.1016/j.fsi.2014.02.023 Polinski, M and Bridle, A and Neumann, L and Nowak, B, Preliminary evidence of transcriptional immunomodulation by praziquantel in bluefin tuna and Atlantic salmon in vitro cultures, Fish and Shellfish Immunology, 38, (1) pp. 42-46. ISSN 1050-4648 (2014) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/24632044 http://ecite.utas.edu.au/91204 Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Refereed Article PeerReviewed 2014 ftunivtasecite https://doi.org/10.1016/j.fsi.2014.02.023 2019-12-13T21:54:26Z Praziquantel (PZQ), long-used in veterinary and human medicine for the treatment of helminth parasites, is known to enhance humoral and cellular immune responsiveness in mammals but has unknown direct immunomodulatory capabilities in fish. In the present study, we examined the ability of PZQ to induce gene transcriptional changes in immune-competent primary tissue/organ cultures of two highly important yet evolutionarily discrete fish species Southern bluefin tuna Thunnus maccoyii and Atlantic salmon Salmo salar . These cultures consisted of mixed blood cell population for both species, as well as intestinal explants from bluefin. Although expression profiles varied between species and tissue/organ type, PZQ induced both T-cell receptor (more than twofold) and IL-8 transcriptional expression (more than fourfold). Additionally, increased expression of other inflammatory cytokines including IL-1β was detected in blood cell cultures from both species, and a general pattern of heightened antiviral signaling was observed. Specifically, elevated transcription of Type I (IFNα) and Type II (IFNγ) interferon in Atlantic salmon blood cultures along with elevated expression of MHC class I in blood cultures of both species. These findings provide preliminary evidence for direct immunomodulation by PZQ in fish and insight into its potential capacity as an immune stimulant/adjuvant in the rapidly expanding aquaculture industry. Article in Journal/Newspaper Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) Fish & Shellfish Immunology 38 1 42 46 |
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eCite UTAS (University of Tasmania) |
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English |
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Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
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Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Polinski, M Bridle, A Neumann, L Nowak, B Preliminary evidence of transcriptional immunomodulation by praziquantel in bluefin tuna and Atlantic salmon in vitro cultures |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
description |
Praziquantel (PZQ), long-used in veterinary and human medicine for the treatment of helminth parasites, is known to enhance humoral and cellular immune responsiveness in mammals but has unknown direct immunomodulatory capabilities in fish. In the present study, we examined the ability of PZQ to induce gene transcriptional changes in immune-competent primary tissue/organ cultures of two highly important yet evolutionarily discrete fish species Southern bluefin tuna Thunnus maccoyii and Atlantic salmon Salmo salar . These cultures consisted of mixed blood cell population for both species, as well as intestinal explants from bluefin. Although expression profiles varied between species and tissue/organ type, PZQ induced both T-cell receptor (more than twofold) and IL-8 transcriptional expression (more than fourfold). Additionally, increased expression of other inflammatory cytokines including IL-1β was detected in blood cell cultures from both species, and a general pattern of heightened antiviral signaling was observed. Specifically, elevated transcription of Type I (IFNα) and Type II (IFNγ) interferon in Atlantic salmon blood cultures along with elevated expression of MHC class I in blood cultures of both species. These findings provide preliminary evidence for direct immunomodulation by PZQ in fish and insight into its potential capacity as an immune stimulant/adjuvant in the rapidly expanding aquaculture industry. |
format |
Article in Journal/Newspaper |
author |
Polinski, M Bridle, A Neumann, L Nowak, B |
author_facet |
Polinski, M Bridle, A Neumann, L Nowak, B |
author_sort |
Polinski, M |
title |
Preliminary evidence of transcriptional immunomodulation by praziquantel in bluefin tuna and Atlantic salmon in vitro cultures |
title_short |
Preliminary evidence of transcriptional immunomodulation by praziquantel in bluefin tuna and Atlantic salmon in vitro cultures |
title_full |
Preliminary evidence of transcriptional immunomodulation by praziquantel in bluefin tuna and Atlantic salmon in vitro cultures |
title_fullStr |
Preliminary evidence of transcriptional immunomodulation by praziquantel in bluefin tuna and Atlantic salmon in vitro cultures |
title_full_unstemmed |
Preliminary evidence of transcriptional immunomodulation by praziquantel in bluefin tuna and Atlantic salmon in vitro cultures |
title_sort |
preliminary evidence of transcriptional immunomodulation by praziquantel in bluefin tuna and atlantic salmon in vitro cultures |
publisher |
Academic Press Ltd Elsevier Science Ltd |
publishDate |
2014 |
url |
https://doi.org/10.1016/j.fsi.2014.02.023 http://www.ncbi.nlm.nih.gov/pubmed/24632044 http://ecite.utas.edu.au/91204 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://dx.doi.org/10.1016/j.fsi.2014.02.023 Polinski, M and Bridle, A and Neumann, L and Nowak, B, Preliminary evidence of transcriptional immunomodulation by praziquantel in bluefin tuna and Atlantic salmon in vitro cultures, Fish and Shellfish Immunology, 38, (1) pp. 42-46. ISSN 1050-4648 (2014) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/24632044 http://ecite.utas.edu.au/91204 |
op_doi |
https://doi.org/10.1016/j.fsi.2014.02.023 |
container_title |
Fish & Shellfish Immunology |
container_volume |
38 |
container_issue |
1 |
container_start_page |
42 |
op_container_end_page |
46 |
_version_ |
1766361199953313792 |