Laser-capture dissection and immunohistochemistry reveals chloride and mucous-cell specific gene expression in gills of seawater acclimated Atlantic salmon Salmo salar

Laser-capture microdissection and immunohistochemistry were used to show that gene and protein expression varied in different cell types in the gills of Atlantic salmon Salmo salar, with chloride cells found to express high levels of sodium potassium ATPase and mucous cells expressing elevated level...

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Bibliographic Details
Published in:Journal of Fish Biology
Main Authors: Nowak, BF, Cadoret, K, Feist, SW, Bean, TP
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2013
Subjects:
Agricultural and Veterinary Sciences
Fisheries Sciences
Aquaculture
Atlantic salmon
Salmo salar
Online Access:https://doi.org/10.1111/jfb.12235
http://www.ncbi.nlm.nih.gov/pubmed/24131303
http://ecite.utas.edu.au/88000
id ftunivtasecite:oai:ecite.utas.edu.au:88000
record_format openpolar
spelling ftunivtasecite:oai:ecite.utas.edu.au:88000 2023-05-15T15:30:04+02:00 Laser-capture dissection and immunohistochemistry reveals chloride and mucous-cell specific gene expression in gills of seawater acclimated Atlantic salmon Salmo salar Nowak, BF Cadoret, K Feist, SW Bean, TP 2013 https://doi.org/10.1111/jfb.12235 http://www.ncbi.nlm.nih.gov/pubmed/24131303 http://ecite.utas.edu.au/88000 en eng Wiley http://dx.doi.org/10.1111/jfb.12235 Nowak, BF and Cadoret, K and Feist, SW and Bean, TP, Laser-capture dissection and immunohistochemistry reveals chloride and mucous-cell specific gene expression in gills of seawater acclimated Atlantic salmon Salmo salar , Journal of Fish Biology, 83, (5) pp. 1459-1467. ISSN 1095-8649 (2013) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/24131303 http://ecite.utas.edu.au/88000 Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Refereed Article PeerReviewed 2013 ftunivtasecite https://doi.org/10.1111/jfb.12235 2019-12-13T21:51:45Z Laser-capture microdissection and immunohistochemistry were used to show that gene and protein expression varied in different cell types in the gills of Atlantic salmon Salmo salar, with chloride cells found to express high levels of sodium potassium ATPase and mucous cells expressing elevated levels of anterior gradient protein. It is therefore important that studies of gene expression in gill tissue take account of the proportion of the various cell types present. Article in Journal/Newspaper Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) Journal of Fish Biology 83 5 1459 1467
institution Open Polar
collection eCite UTAS (University of Tasmania)
op_collection_id ftunivtasecite
language English
topic Agricultural and Veterinary Sciences
Fisheries Sciences
Aquaculture
spellingShingle Agricultural and Veterinary Sciences
Fisheries Sciences
Aquaculture
Nowak, BF
Cadoret, K
Feist, SW
Bean, TP
Laser-capture dissection and immunohistochemistry reveals chloride and mucous-cell specific gene expression in gills of seawater acclimated Atlantic salmon Salmo salar
topic_facet Agricultural and Veterinary Sciences
Fisheries Sciences
Aquaculture
description Laser-capture microdissection and immunohistochemistry were used to show that gene and protein expression varied in different cell types in the gills of Atlantic salmon Salmo salar, with chloride cells found to express high levels of sodium potassium ATPase and mucous cells expressing elevated levels of anterior gradient protein. It is therefore important that studies of gene expression in gill tissue take account of the proportion of the various cell types present.
format Article in Journal/Newspaper
author Nowak, BF
Cadoret, K
Feist, SW
Bean, TP
author_facet Nowak, BF
Cadoret, K
Feist, SW
Bean, TP
author_sort Nowak, BF
title Laser-capture dissection and immunohistochemistry reveals chloride and mucous-cell specific gene expression in gills of seawater acclimated Atlantic salmon Salmo salar
title_short Laser-capture dissection and immunohistochemistry reveals chloride and mucous-cell specific gene expression in gills of seawater acclimated Atlantic salmon Salmo salar
title_full Laser-capture dissection and immunohistochemistry reveals chloride and mucous-cell specific gene expression in gills of seawater acclimated Atlantic salmon Salmo salar
title_fullStr Laser-capture dissection and immunohistochemistry reveals chloride and mucous-cell specific gene expression in gills of seawater acclimated Atlantic salmon Salmo salar
title_full_unstemmed Laser-capture dissection and immunohistochemistry reveals chloride and mucous-cell specific gene expression in gills of seawater acclimated Atlantic salmon Salmo salar
title_sort laser-capture dissection and immunohistochemistry reveals chloride and mucous-cell specific gene expression in gills of seawater acclimated atlantic salmon salmo salar
publisher Wiley
publishDate 2013
url https://doi.org/10.1111/jfb.12235
http://www.ncbi.nlm.nih.gov/pubmed/24131303
http://ecite.utas.edu.au/88000
genre Atlantic salmon
Salmo salar
genre_facet Atlantic salmon
Salmo salar
op_relation http://dx.doi.org/10.1111/jfb.12235
Nowak, BF and Cadoret, K and Feist, SW and Bean, TP, Laser-capture dissection and immunohistochemistry reveals chloride and mucous-cell specific gene expression in gills of seawater acclimated Atlantic salmon Salmo salar , Journal of Fish Biology, 83, (5) pp. 1459-1467. ISSN 1095-8649 (2013) [Refereed Article]
http://www.ncbi.nlm.nih.gov/pubmed/24131303
http://ecite.utas.edu.au/88000
op_doi https://doi.org/10.1111/jfb.12235
container_title Journal of Fish Biology
container_volume 83
container_issue 5
container_start_page 1459
op_container_end_page 1467
_version_ 1766360509768007680

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