Potency of pinnatoxins produced by dinoflagellates isolated from New Zealand and South Australia

• Main Authors: Rhodes, L, Smith, K, Munday, R, Hallegraeff, GM, Selwood, A, Molenaar, S, McNabb, P, Adamson, J, Wilkinson, C
• Format: Conference Object
• Language: English
• Published: International Society for the Study of Harmful Algae and Intergovernmental Oceanographic Commission of UNESCO 2013
• Subjects: Biological Sciences; Plant Biology; Phycology (incl. Marine Grasses); Gand; New Zealand; Pacific; Pacific oyster
• Online Access: http://www.issha.org/Welcome-to-ISSHA/Conferences/ICHA-conference-proceedings/ICHA14-Proceedings; http://ecite.utas.edu.au/86695

Vulcanodinium rugosum., the scrippsielloid dinoflagellate producer of pinnatoxins, wasisolated from surface sediments collected from Northland, New Zealand, in summer2008/2009. The dinoflagellate was isolated and cultured predominantly in cyst form, with theNew Zealand isolates producing pinnatoxins E and F and Australian isolates predominantly Gand less E and F. V. rugosum killed sea urchin and paua larvae and caused morbitity in seaslug larvae; Pacific oyster and Artemia salina larvae became slightly moribund. GreenshellTMmussel larvae were unaffected. Depuration rates of 50% of pinnatoxin E and F from Pacificoysters were 14.5 and 16 d respectively. Crude extracts of mass cultures of the New Zealandand Australian isolates tested for toxicity in mice by intraperitoneal injection, gavage andvoluntary consumption resulted in toxicity ratios of 1.0: 1.8: 4.5 and 1.0: 2.9: 7.8 respectively.