Effect of immunization route on mucosal and systemic immune response in Atlantic salmon ( Salmo salar )
This study aimed to assess systemic and mucosal immune responses of Atlantic salmon ( Salmo salar ) exposed to two protein-hapten antigens dinitrophenol (DNP) and fluorescein isothiocyanate (FITC) each conjugated with keyhole limpet haemocyanin (KLH) administered using different delivery strategies....
Published in: | Veterinary Immunology and Immunopathology |
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Online Access: | https://doi.org/10.1016/j.vetimm.2012.10.010 http://www.ncbi.nlm.nih.gov/pubmed/23206403 http://ecite.utas.edu.au/86381 |
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ftunivtasecite:oai:ecite.utas.edu.au:86381 2023-05-15T15:30:52+02:00 Effect of immunization route on mucosal and systemic immune response in Atlantic salmon ( Salmo salar ) Valdenegro-Vega, VA Crosbie, P Vincent, B Cain, KD Nowak, BF 2013 https://doi.org/10.1016/j.vetimm.2012.10.010 http://www.ncbi.nlm.nih.gov/pubmed/23206403 http://ecite.utas.edu.au/86381 en eng Elsevier Science Bv http://dx.doi.org/10.1016/j.vetimm.2012.10.010 Valdenegro-Vega, VA and Crosbie, P and Vincent, B and Cain, KD and Nowak, BF, Effect of immunization route on mucosal and systemic immune response in Atlantic salmon ( Salmo salar ), Veterinary Immunology and Immunopathology, 151, (1-2) pp. 113-123. ISSN 0165-2427 (2013) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/23206403 http://ecite.utas.edu.au/86381 Agricultural Veterinary and Food Sciences Fisheries sciences Fish pests and diseases Refereed Article PeerReviewed 2013 ftunivtasecite https://doi.org/10.1016/j.vetimm.2012.10.010 2021-03-22T23:16:29Z This study aimed to assess systemic and mucosal immune responses of Atlantic salmon ( Salmo salar ) exposed to two protein-hapten antigens dinitrophenol (DNP) and fluorescein isothiocyanate (FITC) each conjugated with keyhole limpet haemocyanin (KLH) administered using different delivery strategies. Fish were exposed to the antigens through different routes, and were given a booster 4 weeks post initial exposure. Both systemic and mucosal antibody responses were measured for a period of 12 weeks using an enzyme-linked immunosorbent assay (ELISA). Only fish exposed to both antigens via intraperitoneal (IP) injection showed increased systemic antibody response starting 6 weeks post immunization. No treatment was able to produce a mucosal antibody response; however there was an increase in antibody levels in the tissue supernatant from skin explants obtained 12 weeks post immunization from fish injected with FITC. Western blots probed with serum and culture supernatant from skin explants showed a specific response against the antigens. In conclusion, IP injection of hapten-antigen in Atlantic salmon was the best delivery route for inducing an antibody response against these antigens in this species. Even though IP injection did not induce an increase in antibody levels in the skin mucus, there was an increased systemic antibody response and an apparent increase of antibody production in mucosal tissues as demonstrated by the increased level of specific antibody levels in supernatants from the tissue explants. Article in Journal/Newspaper Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) Keyhole ENVELOPE(-67.338,-67.338,-68.785,-68.785) Veterinary Immunology and Immunopathology 151 1-2 113 123 |
institution |
Open Polar |
collection |
eCite UTAS (University of Tasmania) |
op_collection_id |
ftunivtasecite |
language |
English |
topic |
Agricultural Veterinary and Food Sciences Fisheries sciences Fish pests and diseases |
spellingShingle |
Agricultural Veterinary and Food Sciences Fisheries sciences Fish pests and diseases Valdenegro-Vega, VA Crosbie, P Vincent, B Cain, KD Nowak, BF Effect of immunization route on mucosal and systemic immune response in Atlantic salmon ( Salmo salar ) |
topic_facet |
Agricultural Veterinary and Food Sciences Fisheries sciences Fish pests and diseases |
description |
This study aimed to assess systemic and mucosal immune responses of Atlantic salmon ( Salmo salar ) exposed to two protein-hapten antigens dinitrophenol (DNP) and fluorescein isothiocyanate (FITC) each conjugated with keyhole limpet haemocyanin (KLH) administered using different delivery strategies. Fish were exposed to the antigens through different routes, and were given a booster 4 weeks post initial exposure. Both systemic and mucosal antibody responses were measured for a period of 12 weeks using an enzyme-linked immunosorbent assay (ELISA). Only fish exposed to both antigens via intraperitoneal (IP) injection showed increased systemic antibody response starting 6 weeks post immunization. No treatment was able to produce a mucosal antibody response; however there was an increase in antibody levels in the tissue supernatant from skin explants obtained 12 weeks post immunization from fish injected with FITC. Western blots probed with serum and culture supernatant from skin explants showed a specific response against the antigens. In conclusion, IP injection of hapten-antigen in Atlantic salmon was the best delivery route for inducing an antibody response against these antigens in this species. Even though IP injection did not induce an increase in antibody levels in the skin mucus, there was an increased systemic antibody response and an apparent increase of antibody production in mucosal tissues as demonstrated by the increased level of specific antibody levels in supernatants from the tissue explants. |
format |
Article in Journal/Newspaper |
author |
Valdenegro-Vega, VA Crosbie, P Vincent, B Cain, KD Nowak, BF |
author_facet |
Valdenegro-Vega, VA Crosbie, P Vincent, B Cain, KD Nowak, BF |
author_sort |
Valdenegro-Vega, VA |
title |
Effect of immunization route on mucosal and systemic immune response in Atlantic salmon ( Salmo salar ) |
title_short |
Effect of immunization route on mucosal and systemic immune response in Atlantic salmon ( Salmo salar ) |
title_full |
Effect of immunization route on mucosal and systemic immune response in Atlantic salmon ( Salmo salar ) |
title_fullStr |
Effect of immunization route on mucosal and systemic immune response in Atlantic salmon ( Salmo salar ) |
title_full_unstemmed |
Effect of immunization route on mucosal and systemic immune response in Atlantic salmon ( Salmo salar ) |
title_sort |
effect of immunization route on mucosal and systemic immune response in atlantic salmon ( salmo salar ) |
publisher |
Elsevier Science Bv |
publishDate |
2013 |
url |
https://doi.org/10.1016/j.vetimm.2012.10.010 http://www.ncbi.nlm.nih.gov/pubmed/23206403 http://ecite.utas.edu.au/86381 |
long_lat |
ENVELOPE(-67.338,-67.338,-68.785,-68.785) |
geographic |
Keyhole |
geographic_facet |
Keyhole |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://dx.doi.org/10.1016/j.vetimm.2012.10.010 Valdenegro-Vega, VA and Crosbie, P and Vincent, B and Cain, KD and Nowak, BF, Effect of immunization route on mucosal and systemic immune response in Atlantic salmon ( Salmo salar ), Veterinary Immunology and Immunopathology, 151, (1-2) pp. 113-123. ISSN 0165-2427 (2013) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/23206403 http://ecite.utas.edu.au/86381 |
op_doi |
https://doi.org/10.1016/j.vetimm.2012.10.010 |
container_title |
Veterinary Immunology and Immunopathology |
container_volume |
151 |
container_issue |
1-2 |
container_start_page |
113 |
op_container_end_page |
123 |
_version_ |
1766361328476225536 |