Molecular analysis of the bacterial communities in the live Pacific oyster ( Crassostrea gigas ) and the influence of postharvest temperature on its structure
Aims: To evaluate the effect of postharvest temperature on bacterial communities in live Pacific oysters ( Crassostrea gigas ) using nonculture-based methods. Methods and Results: Live oysters were compared before and after storage at 4, 6, 15, 20 and 30 Celsius Degree using terminal restriction fra...
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Online Access: | https://doi.org/10.1111/j.1365-2672.2012.05287.x http://www.ncbi.nlm.nih.gov/pubmed/22429335 http://ecite.utas.edu.au/77419 |
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ftunivtasecite:oai:ecite.utas.edu.au:77419 2023-05-15T15:58:15+02:00 Molecular analysis of the bacterial communities in the live Pacific oyster ( Crassostrea gigas ) and the influence of postharvest temperature on its structure Fernandez Piquer, J Bowman, JP Ross, T Tamplin, ML 2012 application/pdf https://doi.org/10.1111/j.1365-2672.2012.05287.x http://www.ncbi.nlm.nih.gov/pubmed/22429335 http://ecite.utas.edu.au/77419 en eng Blackwell Publishing Ltd http://ecite.utas.edu.au/77419/2/PES 77419 Fernandez et al JAM 2012 Final.pdf http://dx.doi.org/10.1111/j.1365-2672.2012.05287.x Fernandez Piquer, J and Bowman, JP and Ross, T and Tamplin, ML, Molecular analysis of the bacterial communities in the live Pacific oyster ( Crassostrea gigas ) and the influence of postharvest temperature on its structure, Journal of Applied Microbiology, 112, (6) pp. 1134-1143. ISSN 1364-5072 (2012) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/22429335 http://ecite.utas.edu.au/77419 Biological Sciences Microbiology Microbiology not elsewhere classified Refereed Article PeerReviewed 2012 ftunivtasecite https://doi.org/10.1111/j.1365-2672.2012.05287.x 2022-08-29T22:16:46Z Aims: To evaluate the effect of postharvest temperature on bacterial communities in live Pacific oysters ( Crassostrea gigas ) using nonculture-based methods. Methods and Results: Live oysters were compared before and after storage at 4, 6, 15, 20 and 30 Celsius Degree using terminal restriction fragment length polymorphism (T-RFLP). Bacterial communities in freshly harvested (control) vs stored oysters were significantly different. Changes in bacterial communities at 4, 15 and 30 Celsius Degree observed by T-RFLP were further investigated by clone library analysis. Members of the Proteobacteria predominated (43.0-57.0% of clones) in control oysters, while storage altered the bacterial profile. At 4 Celsius Degree, Psychrilyobacter spp.(phylum Fusobacteria ) predominated (43.8% of clones), while at 15 and 30 Celsius Degree, members of the phylum Bacteroidetes represented 63.0 and 60.2% of clones, respectively. High microbial diversity in oysters was observed, with at least 73 different genera-related clones among all samples. Conclusions: Changes in the overall bacterial community of Pacific oysters were influenced by storage temperature and would likely not be detected by standard culture-based methods currently used to assess oyster quality. Certain dominant genera, such as Psychrilyobacter , Polynucleobacter and a bacterial group related to Alkaliflexus , should be further studied as possible indicators for postharvest temperature control. Significance and Impact of the Study: This work is the first report describing the effect of different storage temperatures on bacterial diversity in postharvest live Pacific oysters using molecular-based methods. Article in Journal/Newspaper Crassostrea gigas Pacific oyster eCite UTAS (University of Tasmania) Pacific Journal of Applied Microbiology 112 6 1134 1143 |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Biological Sciences Microbiology Microbiology not elsewhere classified |
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Biological Sciences Microbiology Microbiology not elsewhere classified Fernandez Piquer, J Bowman, JP Ross, T Tamplin, ML Molecular analysis of the bacterial communities in the live Pacific oyster ( Crassostrea gigas ) and the influence of postharvest temperature on its structure |
topic_facet |
Biological Sciences Microbiology Microbiology not elsewhere classified |
description |
Aims: To evaluate the effect of postharvest temperature on bacterial communities in live Pacific oysters ( Crassostrea gigas ) using nonculture-based methods. Methods and Results: Live oysters were compared before and after storage at 4, 6, 15, 20 and 30 Celsius Degree using terminal restriction fragment length polymorphism (T-RFLP). Bacterial communities in freshly harvested (control) vs stored oysters were significantly different. Changes in bacterial communities at 4, 15 and 30 Celsius Degree observed by T-RFLP were further investigated by clone library analysis. Members of the Proteobacteria predominated (43.0-57.0% of clones) in control oysters, while storage altered the bacterial profile. At 4 Celsius Degree, Psychrilyobacter spp.(phylum Fusobacteria ) predominated (43.8% of clones), while at 15 and 30 Celsius Degree, members of the phylum Bacteroidetes represented 63.0 and 60.2% of clones, respectively. High microbial diversity in oysters was observed, with at least 73 different genera-related clones among all samples. Conclusions: Changes in the overall bacterial community of Pacific oysters were influenced by storage temperature and would likely not be detected by standard culture-based methods currently used to assess oyster quality. Certain dominant genera, such as Psychrilyobacter , Polynucleobacter and a bacterial group related to Alkaliflexus , should be further studied as possible indicators for postharvest temperature control. Significance and Impact of the Study: This work is the first report describing the effect of different storage temperatures on bacterial diversity in postharvest live Pacific oysters using molecular-based methods. |
format |
Article in Journal/Newspaper |
author |
Fernandez Piquer, J Bowman, JP Ross, T Tamplin, ML |
author_facet |
Fernandez Piquer, J Bowman, JP Ross, T Tamplin, ML |
author_sort |
Fernandez Piquer, J |
title |
Molecular analysis of the bacterial communities in the live Pacific oyster ( Crassostrea gigas ) and the influence of postharvest temperature on its structure |
title_short |
Molecular analysis of the bacterial communities in the live Pacific oyster ( Crassostrea gigas ) and the influence of postharvest temperature on its structure |
title_full |
Molecular analysis of the bacterial communities in the live Pacific oyster ( Crassostrea gigas ) and the influence of postharvest temperature on its structure |
title_fullStr |
Molecular analysis of the bacterial communities in the live Pacific oyster ( Crassostrea gigas ) and the influence of postharvest temperature on its structure |
title_full_unstemmed |
Molecular analysis of the bacterial communities in the live Pacific oyster ( Crassostrea gigas ) and the influence of postharvest temperature on its structure |
title_sort |
molecular analysis of the bacterial communities in the live pacific oyster ( crassostrea gigas ) and the influence of postharvest temperature on its structure |
publisher |
Blackwell Publishing Ltd |
publishDate |
2012 |
url |
https://doi.org/10.1111/j.1365-2672.2012.05287.x http://www.ncbi.nlm.nih.gov/pubmed/22429335 http://ecite.utas.edu.au/77419 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
http://ecite.utas.edu.au/77419/2/PES 77419 Fernandez et al JAM 2012 Final.pdf http://dx.doi.org/10.1111/j.1365-2672.2012.05287.x Fernandez Piquer, J and Bowman, JP and Ross, T and Tamplin, ML, Molecular analysis of the bacterial communities in the live Pacific oyster ( Crassostrea gigas ) and the influence of postharvest temperature on its structure, Journal of Applied Microbiology, 112, (6) pp. 1134-1143. ISSN 1364-5072 (2012) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/22429335 http://ecite.utas.edu.au/77419 |
op_doi |
https://doi.org/10.1111/j.1365-2672.2012.05287.x |
container_title |
Journal of Applied Microbiology |
container_volume |
112 |
container_issue |
6 |
container_start_page |
1134 |
op_container_end_page |
1143 |
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1766393977521569792 |