Validation and quantification of extractable age pigments for determining the age of Antarctic krill (Euphausia superba)
Antarctic krill, Euphausia superba, hatchedfrom eggs and maintained for four years, were sampledperiodically for age-pigment analysis. Extractable pigmentsfrom the eye and eyestalk ganglia were quantifiedusing fluorescence intensity and standardised against protein.Three peak fluorescence intensitie...
Published in: | Marine Biology |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Springer-Verlag
2011
|
Subjects: | |
Online Access: | https://doi.org/10.1007/s00227-011-1688-5 http://ecite.utas.edu.au/76372 |
id |
ftunivtasecite:oai:ecite.utas.edu.au:76372 |
---|---|
record_format |
openpolar |
spelling |
ftunivtasecite:oai:ecite.utas.edu.au:76372 2023-05-15T14:02:30+02:00 Validation and quantification of extractable age pigments for determining the age of Antarctic krill (Euphausia superba) McGaffin, AF Nicol, S Virtue, P Hirano, Y Matsuda, T Uchida, I Candy, SG Kawaguchi, S 2011 application/pdf https://doi.org/10.1007/s00227-011-1688-5 http://ecite.utas.edu.au/76372 en eng Springer-Verlag http://ecite.utas.edu.au/76372/1/Ange's paper.pdf http://dx.doi.org/10.1007/s00227-011-1688-5 McGaffin, AF and Nicol, S and Virtue, P and Hirano, Y and Matsuda, T and Uchida, I and Candy, SG and Kawaguchi, S, Validation and quantification of extractable age pigments for determining the age of Antarctic krill (Euphausia superba) , Marine Biology: International Journal on Life in Oceans and Coastal Waters, 158, (8) pp. 1743-1755. ISSN 0025-3162 (2011) [Refereed Article] http://ecite.utas.edu.au/76372 Biological Sciences Ecology Marine and Estuarine Ecology (incl. Marine Ichthyology) Refereed Article PeerReviewed 2011 ftunivtasecite https://doi.org/10.1007/s00227-011-1688-5 2019-12-13T21:42:45Z Antarctic krill, Euphausia superba, hatchedfrom eggs and maintained for four years, were sampledperiodically for age-pigment analysis. Extractable pigmentsfrom the eye and eyestalk ganglia were quantifiedusing fluorescence intensity and standardised against protein.Three peak fluorescence intensities were detected atwavelengths of excitation 280 nm, emission 625 nm (pigment1); excitation 355 nm, emission 510 nm (pigment 2);and excitation 463 nm, emission 620 nm (pigment 3).There was a positive correlation between the quantity ofpigments 1 and 3 and the age of Antarctic krill. A modelwas developed to predict age from pigment 3 and tocompare it with other age proxies (carapace length andeyeball diameter). The quantity of pigment 3 was the bestpredictor of age. The pigment method can discriminatebetween similar sized krill aged 12 and 36 months. Agepigments provide an improved tool for age estimation inAntarctic krill, particularly if used in conjunction withother demographic information. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Euphausia superba eCite UTAS (University of Tasmania) Antarctic Marine Biology 158 8 1743 1755 |
institution |
Open Polar |
collection |
eCite UTAS (University of Tasmania) |
op_collection_id |
ftunivtasecite |
language |
English |
topic |
Biological Sciences Ecology Marine and Estuarine Ecology (incl. Marine Ichthyology) |
spellingShingle |
Biological Sciences Ecology Marine and Estuarine Ecology (incl. Marine Ichthyology) McGaffin, AF Nicol, S Virtue, P Hirano, Y Matsuda, T Uchida, I Candy, SG Kawaguchi, S Validation and quantification of extractable age pigments for determining the age of Antarctic krill (Euphausia superba) |
topic_facet |
Biological Sciences Ecology Marine and Estuarine Ecology (incl. Marine Ichthyology) |
description |
Antarctic krill, Euphausia superba, hatchedfrom eggs and maintained for four years, were sampledperiodically for age-pigment analysis. Extractable pigmentsfrom the eye and eyestalk ganglia were quantifiedusing fluorescence intensity and standardised against protein.Three peak fluorescence intensities were detected atwavelengths of excitation 280 nm, emission 625 nm (pigment1); excitation 355 nm, emission 510 nm (pigment 2);and excitation 463 nm, emission 620 nm (pigment 3).There was a positive correlation between the quantity ofpigments 1 and 3 and the age of Antarctic krill. A modelwas developed to predict age from pigment 3 and tocompare it with other age proxies (carapace length andeyeball diameter). The quantity of pigment 3 was the bestpredictor of age. The pigment method can discriminatebetween similar sized krill aged 12 and 36 months. Agepigments provide an improved tool for age estimation inAntarctic krill, particularly if used in conjunction withother demographic information. |
format |
Article in Journal/Newspaper |
author |
McGaffin, AF Nicol, S Virtue, P Hirano, Y Matsuda, T Uchida, I Candy, SG Kawaguchi, S |
author_facet |
McGaffin, AF Nicol, S Virtue, P Hirano, Y Matsuda, T Uchida, I Candy, SG Kawaguchi, S |
author_sort |
McGaffin, AF |
title |
Validation and quantification of extractable age pigments for determining the age of Antarctic krill (Euphausia superba) |
title_short |
Validation and quantification of extractable age pigments for determining the age of Antarctic krill (Euphausia superba) |
title_full |
Validation and quantification of extractable age pigments for determining the age of Antarctic krill (Euphausia superba) |
title_fullStr |
Validation and quantification of extractable age pigments for determining the age of Antarctic krill (Euphausia superba) |
title_full_unstemmed |
Validation and quantification of extractable age pigments for determining the age of Antarctic krill (Euphausia superba) |
title_sort |
validation and quantification of extractable age pigments for determining the age of antarctic krill (euphausia superba) |
publisher |
Springer-Verlag |
publishDate |
2011 |
url |
https://doi.org/10.1007/s00227-011-1688-5 http://ecite.utas.edu.au/76372 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba |
op_relation |
http://ecite.utas.edu.au/76372/1/Ange's paper.pdf http://dx.doi.org/10.1007/s00227-011-1688-5 McGaffin, AF and Nicol, S and Virtue, P and Hirano, Y and Matsuda, T and Uchida, I and Candy, SG and Kawaguchi, S, Validation and quantification of extractable age pigments for determining the age of Antarctic krill (Euphausia superba) , Marine Biology: International Journal on Life in Oceans and Coastal Waters, 158, (8) pp. 1743-1755. ISSN 0025-3162 (2011) [Refereed Article] http://ecite.utas.edu.au/76372 |
op_doi |
https://doi.org/10.1007/s00227-011-1688-5 |
container_title |
Marine Biology |
container_volume |
158 |
container_issue |
8 |
container_start_page |
1743 |
op_container_end_page |
1755 |
_version_ |
1766272787274530816 |