Studies on triploid oysters in Australia. VII. Assessment of two methods for determining triploidy in oysters: adductor muscle diameter and nuclear size
Two methods for distinguishing triploid Pacific oysters [Crassostrea gigas (Thunberg)] from diploid oysters were assessed. Adductor muscle diameter in relation to valve height was significantly (p < 0.001) greater in samples of triploid oysters than in diploid samples and was influenced by site....
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1996
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ftunivtasecite:oai:ecite.utas.edu.au:7607 2023-05-15T15:58:35+02:00 Studies on triploid oysters in Australia. VII. Assessment of two methods for determining triploidy in oysters: adductor muscle diameter and nuclear size Gardner, C Maguire, GB Kent, GN 1996 http://ecite.utas.edu.au/7607 en eng Natl Shellfisheries Assoc Gardner, C and Maguire, GB and Kent, GN, Studies on triploid oysters in Australia. VII. Assessment of two methods for determining triploidy in oysters: adductor muscle diameter and nuclear size, Journal of Shellfish Research, 15, (3) pp. 609-615. ISSN 0730-8000 (1996) [Refereed Article] http://ecite.utas.edu.au/7607 Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Refereed Article PeerReviewed 1996 ftunivtasecite 2019-12-13T20:54:16Z Two methods for distinguishing triploid Pacific oysters [Crassostrea gigas (Thunberg)] from diploid oysters were assessed. Adductor muscle diameter in relation to valve height was significantly (p < 0.001) greater in samples of triploid oysters than in diploid samples and was influenced by site. However, variation in this measure was too large to allow individual oysters to be distinguished as either triploid or diploid. A second method was assessed that used differences in the nuclear size of hemocytes and the intensity of staining of hemocyte nuclei to distinguish between diploids and triploids. Histological sections, prepared by standard paraffin histology, were stained for nuclear histones with Gill's hematoxylin. Integrated nuclear optical density and nuclear area were recorded with image analysis. This method was effective in distinguishing individual oysters as diploid or triploid. When histological specimens are required, this method is less expensive than other techniques used to determine triploidy. Article in Journal/Newspaper Crassostrea gigas eCite UTAS (University of Tasmania) Pacific |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
spellingShingle |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Gardner, C Maguire, GB Kent, GN Studies on triploid oysters in Australia. VII. Assessment of two methods for determining triploidy in oysters: adductor muscle diameter and nuclear size |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
description |
Two methods for distinguishing triploid Pacific oysters [Crassostrea gigas (Thunberg)] from diploid oysters were assessed. Adductor muscle diameter in relation to valve height was significantly (p < 0.001) greater in samples of triploid oysters than in diploid samples and was influenced by site. However, variation in this measure was too large to allow individual oysters to be distinguished as either triploid or diploid. A second method was assessed that used differences in the nuclear size of hemocytes and the intensity of staining of hemocyte nuclei to distinguish between diploids and triploids. Histological sections, prepared by standard paraffin histology, were stained for nuclear histones with Gill's hematoxylin. Integrated nuclear optical density and nuclear area were recorded with image analysis. This method was effective in distinguishing individual oysters as diploid or triploid. When histological specimens are required, this method is less expensive than other techniques used to determine triploidy. |
format |
Article in Journal/Newspaper |
author |
Gardner, C Maguire, GB Kent, GN |
author_facet |
Gardner, C Maguire, GB Kent, GN |
author_sort |
Gardner, C |
title |
Studies on triploid oysters in Australia. VII. Assessment of two methods for determining triploidy in oysters: adductor muscle diameter and nuclear size |
title_short |
Studies on triploid oysters in Australia. VII. Assessment of two methods for determining triploidy in oysters: adductor muscle diameter and nuclear size |
title_full |
Studies on triploid oysters in Australia. VII. Assessment of two methods for determining triploidy in oysters: adductor muscle diameter and nuclear size |
title_fullStr |
Studies on triploid oysters in Australia. VII. Assessment of two methods for determining triploidy in oysters: adductor muscle diameter and nuclear size |
title_full_unstemmed |
Studies on triploid oysters in Australia. VII. Assessment of two methods for determining triploidy in oysters: adductor muscle diameter and nuclear size |
title_sort |
studies on triploid oysters in australia. vii. assessment of two methods for determining triploidy in oysters: adductor muscle diameter and nuclear size |
publisher |
Natl Shellfisheries Assoc |
publishDate |
1996 |
url |
http://ecite.utas.edu.au/7607 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
Gardner, C and Maguire, GB and Kent, GN, Studies on triploid oysters in Australia. VII. Assessment of two methods for determining triploidy in oysters: adductor muscle diameter and nuclear size, Journal of Shellfish Research, 15, (3) pp. 609-615. ISSN 0730-8000 (1996) [Refereed Article] http://ecite.utas.edu.au/7607 |
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