Proteorhodospin-bearing bacteria in Antarctic sea ice
Proteorhodopsins (PRs) are widespread bacterial integral membrane proteins that function as light-drivenproton pumps. Antarctic sea ice supports a complex community of autotrophic algae, heterotrophic bacteria,viruses, and protists that are an important food source for higher trophic levels in ice-c...
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ftunivtasecite:oai:ecite.utas.edu.au:68331 2023-05-15T13:35:38+02:00 Proteorhodospin-bearing bacteria in Antarctic sea ice Koh, EY Atamna-Ismaeel, N Martin, AR Cowie, ROM Beja, O Davy, SK Maas, EW Ryan, KG 2010 application/pdf http://aem.asm.org/cgi/content/abstract/76/17/5918 https://doi.org/10.1128/AEM.00562-10 http://www.ncbi.nlm.nih.gov/pubmed/20601510 http://ecite.utas.edu.au/68331 en eng Amer Soc Microbiology http://ecite.utas.edu.au/68331/1/Koh et al, 2010 - PR-bearing bacteria in antarctic sea ice.pdf http://dx.doi.org/10.1128/AEM.00562-10 Koh, EY and Atamna-Ismaeel, N and Martin, AR and Cowie, ROM and Beja, O and Davy, SK and Maas, EW and Ryan, KG, Proteorhodospin-bearing bacteria in Antarctic sea ice, Applied and Environmental Microbiology, 76, (17) pp. 5918-5925. ISSN 0099-2240 (2010) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/20601510 http://ecite.utas.edu.au/68331 Biological Sciences Microbiology Microbial Ecology Refereed Article PeerReviewed 2010 ftunivtasecite https://doi.org/10.1128/AEM.00562-10 2019-12-13T21:36:45Z Proteorhodopsins (PRs) are widespread bacterial integral membrane proteins that function as light-drivenproton pumps. Antarctic sea ice supports a complex community of autotrophic algae, heterotrophic bacteria,viruses, and protists that are an important food source for higher trophic levels in ice-covered regions of theSouthern Ocean. Here, we present the first report of PR-bearing bacteria, both dormant and active, inAntarctic sea ice from a series of sites in the Ross Sea using gene-specific primers. Positive PR sequences weregenerated from genomic DNA at all depths in sea ice, and these sequences aligned with the classes Alphaproteobacteria,Gammaproteobacteria, and Flavobacteria. The sequences showed some similarity to previouslyreported PR sequences, although most of the sequences were generally distinct. Positive PR sequences werealso observed from cDNA reverse transcribed from RNA isolated from sea ice samples. This finding indicatesthat these sequences were generated from metabolically active cells and suggests that the PR gene is functionalwithin sea ice. Both blue-absorbing and green-absorbing forms of PRs were detected, and only a limitednumber of blue-absorbing forms were found and were in the midsection of the sea ice profile in this study.Questions still remain regarding the proteins ecological functions, and ultimately, field experiments will beneeded to establish the ecological and functional role of PRs in the sea ice ecosystem. Article in Journal/Newspaper Antarc* Antarctic Ross Sea Sea ice eCite UTAS (University of Tasmania) Antarctic Ross Sea Applied and Environmental Microbiology 76 17 5918 5925 |
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Open Polar |
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eCite UTAS (University of Tasmania) |
op_collection_id |
ftunivtasecite |
language |
English |
topic |
Biological Sciences Microbiology Microbial Ecology |
spellingShingle |
Biological Sciences Microbiology Microbial Ecology Koh, EY Atamna-Ismaeel, N Martin, AR Cowie, ROM Beja, O Davy, SK Maas, EW Ryan, KG Proteorhodospin-bearing bacteria in Antarctic sea ice |
topic_facet |
Biological Sciences Microbiology Microbial Ecology |
description |
Proteorhodopsins (PRs) are widespread bacterial integral membrane proteins that function as light-drivenproton pumps. Antarctic sea ice supports a complex community of autotrophic algae, heterotrophic bacteria,viruses, and protists that are an important food source for higher trophic levels in ice-covered regions of theSouthern Ocean. Here, we present the first report of PR-bearing bacteria, both dormant and active, inAntarctic sea ice from a series of sites in the Ross Sea using gene-specific primers. Positive PR sequences weregenerated from genomic DNA at all depths in sea ice, and these sequences aligned with the classes Alphaproteobacteria,Gammaproteobacteria, and Flavobacteria. The sequences showed some similarity to previouslyreported PR sequences, although most of the sequences were generally distinct. Positive PR sequences werealso observed from cDNA reverse transcribed from RNA isolated from sea ice samples. This finding indicatesthat these sequences were generated from metabolically active cells and suggests that the PR gene is functionalwithin sea ice. Both blue-absorbing and green-absorbing forms of PRs were detected, and only a limitednumber of blue-absorbing forms were found and were in the midsection of the sea ice profile in this study.Questions still remain regarding the proteins ecological functions, and ultimately, field experiments will beneeded to establish the ecological and functional role of PRs in the sea ice ecosystem. |
format |
Article in Journal/Newspaper |
author |
Koh, EY Atamna-Ismaeel, N Martin, AR Cowie, ROM Beja, O Davy, SK Maas, EW Ryan, KG |
author_facet |
Koh, EY Atamna-Ismaeel, N Martin, AR Cowie, ROM Beja, O Davy, SK Maas, EW Ryan, KG |
author_sort |
Koh, EY |
title |
Proteorhodospin-bearing bacteria in Antarctic sea ice |
title_short |
Proteorhodospin-bearing bacteria in Antarctic sea ice |
title_full |
Proteorhodospin-bearing bacteria in Antarctic sea ice |
title_fullStr |
Proteorhodospin-bearing bacteria in Antarctic sea ice |
title_full_unstemmed |
Proteorhodospin-bearing bacteria in Antarctic sea ice |
title_sort |
proteorhodospin-bearing bacteria in antarctic sea ice |
publisher |
Amer Soc Microbiology |
publishDate |
2010 |
url |
http://aem.asm.org/cgi/content/abstract/76/17/5918 https://doi.org/10.1128/AEM.00562-10 http://www.ncbi.nlm.nih.gov/pubmed/20601510 http://ecite.utas.edu.au/68331 |
geographic |
Antarctic Ross Sea |
geographic_facet |
Antarctic Ross Sea |
genre |
Antarc* Antarctic Ross Sea Sea ice |
genre_facet |
Antarc* Antarctic Ross Sea Sea ice |
op_relation |
http://ecite.utas.edu.au/68331/1/Koh et al, 2010 - PR-bearing bacteria in antarctic sea ice.pdf http://dx.doi.org/10.1128/AEM.00562-10 Koh, EY and Atamna-Ismaeel, N and Martin, AR and Cowie, ROM and Beja, O and Davy, SK and Maas, EW and Ryan, KG, Proteorhodospin-bearing bacteria in Antarctic sea ice, Applied and Environmental Microbiology, 76, (17) pp. 5918-5925. ISSN 0099-2240 (2010) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/20601510 http://ecite.utas.edu.au/68331 |
op_doi |
https://doi.org/10.1128/AEM.00562-10 |
container_title |
Applied and Environmental Microbiology |
container_volume |
76 |
container_issue |
17 |
container_start_page |
5918 |
op_container_end_page |
5925 |
_version_ |
1766068358424297472 |