Physical characterisation of Tenacibaculum maritimum for vaccine development
Aims: Tenacibaculum maritimum is a well known fish pathogen worldwide, affecting many fish species including Atlantic salmon in Tasmania, Australia. The aim of this study was to characterise and understand the similarities and differences between the isolates in order to select isolates for later pa...
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Online Access: | https://doi.org/10.1111/j.1365-2672.2010.04795.x http://www.ncbi.nlm.nih.gov/pubmed/20636343 http://ecite.utas.edu.au/65529 |
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ftunivtasecite:oai:ecite.utas.edu.au:65529 2023-05-15T15:32:22+02:00 Physical characterisation of Tenacibaculum maritimum for vaccine development van Gelderen, R Carson, J Gudkovs, N Nowak, B 2010 https://doi.org/10.1111/j.1365-2672.2010.04795.x http://www.ncbi.nlm.nih.gov/pubmed/20636343 http://ecite.utas.edu.au/65529 en eng Blackwell Publishing Ltd http://dx.doi.org/10.1111/j.1365-2672.2010.04795.x van Gelderen, R and Carson, J and Gudkovs, N and Nowak, B, Physical characterisation of Tenacibaculum maritimum for vaccine development, Journal of Applied Microbiology, 109, (5) pp. 1668-1676. ISSN 1364-5072 (2010) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/20636343 http://ecite.utas.edu.au/65529 Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases Refereed Article PeerReviewed 2010 ftunivtasecite https://doi.org/10.1111/j.1365-2672.2010.04795.x 2019-12-13T21:34:50Z Aims: Tenacibaculum maritimum is a well known fish pathogen worldwide, affecting many fish species including Atlantic salmon in Tasmania, Australia. The aim of this study was to characterise and understand the similarities and differences between the isolates in order to select isolates for later pathogenicity and vaccination trials. Methods and Results: Several physical characterisation tests were carried out: whole cell protein profiles, lipopolysaccharide profiles (LPS), extracellular product profiles (ECP), indirect immunofluorescent antibody test (IFAT) and hydrophobicity. The Tasmanian strains of T. maritimum appear relatively homogeneous physically, but antigenically different. All isolates were hydrophobic and produce a variety of ECP profiles. There were two isolates that stand out in all assays (89/4747 and 01/0356-7) and showed great variation from the other isolates. All isolates have been confirmed as T. maritimum . Conclusions: Based on the tests carried out three isolates were chosen for in vivo trials: 89/4747, 89/4762, 00/3280. Significance and Impact of the Study: This is the first study to characterise T. maritimum isolates from Tasmanian waters. The opportunity to develop vaccines for the Tasmanian salmonid aquaculture industry is enhanced by a greater understanding of the physical characteristics of pathogens. Article in Journal/Newspaper Atlantic salmon eCite UTAS (University of Tasmania) Journal of Applied Microbiology no no |
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eCite UTAS (University of Tasmania) |
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English |
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Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases |
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Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases van Gelderen, R Carson, J Gudkovs, N Nowak, B Physical characterisation of Tenacibaculum maritimum for vaccine development |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases |
description |
Aims: Tenacibaculum maritimum is a well known fish pathogen worldwide, affecting many fish species including Atlantic salmon in Tasmania, Australia. The aim of this study was to characterise and understand the similarities and differences between the isolates in order to select isolates for later pathogenicity and vaccination trials. Methods and Results: Several physical characterisation tests were carried out: whole cell protein profiles, lipopolysaccharide profiles (LPS), extracellular product profiles (ECP), indirect immunofluorescent antibody test (IFAT) and hydrophobicity. The Tasmanian strains of T. maritimum appear relatively homogeneous physically, but antigenically different. All isolates were hydrophobic and produce a variety of ECP profiles. There were two isolates that stand out in all assays (89/4747 and 01/0356-7) and showed great variation from the other isolates. All isolates have been confirmed as T. maritimum . Conclusions: Based on the tests carried out three isolates were chosen for in vivo trials: 89/4747, 89/4762, 00/3280. Significance and Impact of the Study: This is the first study to characterise T. maritimum isolates from Tasmanian waters. The opportunity to develop vaccines for the Tasmanian salmonid aquaculture industry is enhanced by a greater understanding of the physical characteristics of pathogens. |
format |
Article in Journal/Newspaper |
author |
van Gelderen, R Carson, J Gudkovs, N Nowak, B |
author_facet |
van Gelderen, R Carson, J Gudkovs, N Nowak, B |
author_sort |
van Gelderen, R |
title |
Physical characterisation of Tenacibaculum maritimum for vaccine development |
title_short |
Physical characterisation of Tenacibaculum maritimum for vaccine development |
title_full |
Physical characterisation of Tenacibaculum maritimum for vaccine development |
title_fullStr |
Physical characterisation of Tenacibaculum maritimum for vaccine development |
title_full_unstemmed |
Physical characterisation of Tenacibaculum maritimum for vaccine development |
title_sort |
physical characterisation of tenacibaculum maritimum for vaccine development |
publisher |
Blackwell Publishing Ltd |
publishDate |
2010 |
url |
https://doi.org/10.1111/j.1365-2672.2010.04795.x http://www.ncbi.nlm.nih.gov/pubmed/20636343 http://ecite.utas.edu.au/65529 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://dx.doi.org/10.1111/j.1365-2672.2010.04795.x van Gelderen, R and Carson, J and Gudkovs, N and Nowak, B, Physical characterisation of Tenacibaculum maritimum for vaccine development, Journal of Applied Microbiology, 109, (5) pp. 1668-1676. ISSN 1364-5072 (2010) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/20636343 http://ecite.utas.edu.au/65529 |
op_doi |
https://doi.org/10.1111/j.1365-2672.2010.04795.x |
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Journal of Applied Microbiology |
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1766362881690959872 |