Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways
Amoebic gill disease (AGD) is a parasite-mediated proliferative gill disease capable of affecting a range of teleost hosts. While a moderate heritability for AGD resistance in Atlantic salmon has been reported previously, the mechanisms by which individuals resist the proliferative effects remain po...
Published in: | Developmental & Comparative Immunology |
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Online Access: | https://doi.org/10.1016/j.dci.2008.05.013 http://www.ncbi.nlm.nih.gov/pubmed/18621418 http://ecite.utas.edu.au/55463 |
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ftunivtasecite:oai:ecite.utas.edu.au:55463 2023-05-15T15:30:50+02:00 Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways Wynne, JW O'Sullivan, MG Stone, G Cook, MT Nowak, BF Lovell, DR Taylor, RS Elliott, NG 2008 https://doi.org/10.1016/j.dci.2008.05.013 http://www.ncbi.nlm.nih.gov/pubmed/18621418 http://ecite.utas.edu.au/55463 en eng Elsevier Sci Ltd http://dx.doi.org/10.1016/j.dci.2008.05.013 Wynne, JW and O'Sullivan, MG and Stone, G and Cook, MT and Nowak, BF and Lovell, DR and Taylor, RS and Elliott, NG, Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways, Developmental and Comparative Immunology, 32, (12) pp. 1539 - 1560. ISSN 0145-305X (2008) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/18621418 http://ecite.utas.edu.au/55463 Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases Refereed Article PeerReviewed 2008 ftunivtasecite https://doi.org/10.1016/j.dci.2008.05.013 2019-12-13T21:28:04Z Amoebic gill disease (AGD) is a parasite-mediated proliferative gill disease capable of affecting a range of teleost hosts. While a moderate heritability for AGD resistance in Atlantic salmon has been reported previously, the mechanisms by which individuals resist the proliferative effects remain poorly understood. To gain more knowledge of this commercially important trait, we compared gill transcriptomes of two groups of Atlantic salmon, one designated putatively resistant, and one designated putatively susceptible to AGD. Utilising a 17k Atlantic salmon cDNA microarray we identified 196 transcripts that were differentially expressed between the two groups. Expression of 11 transcripts were further examined with real-time quantitative RT-PCR (qPCR) in the AGD-resistant and AGD-susceptible animals, as well as non-infected nave fish. Gene expression determined by qPCR was in strong agreement with the microarray analysis. A large number of differentially expressed genes were involved in immune and cell cycle responses. Resistant individuals displayed significantly higher expression of genes involved in adaptive immunity and negative regulation of the cell cycle. In contrast, AGD-susceptible individuals showed higher expression of acute phase proteins and positive regulators of the cell cycle. Combined with the gill histopathology, our results suggest AGD resistance is acquired rather than innately present, and that this resistance is for the most part associated with the dysregulation of immune and cell cycle pathways. 2008 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Atlantic salmon eCite UTAS (University of Tasmania) Developmental & Comparative Immunology 32 12 1539 1560 |
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Open Polar |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases |
spellingShingle |
Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases Wynne, JW O'Sullivan, MG Stone, G Cook, MT Nowak, BF Lovell, DR Taylor, RS Elliott, NG Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases |
description |
Amoebic gill disease (AGD) is a parasite-mediated proliferative gill disease capable of affecting a range of teleost hosts. While a moderate heritability for AGD resistance in Atlantic salmon has been reported previously, the mechanisms by which individuals resist the proliferative effects remain poorly understood. To gain more knowledge of this commercially important trait, we compared gill transcriptomes of two groups of Atlantic salmon, one designated putatively resistant, and one designated putatively susceptible to AGD. Utilising a 17k Atlantic salmon cDNA microarray we identified 196 transcripts that were differentially expressed between the two groups. Expression of 11 transcripts were further examined with real-time quantitative RT-PCR (qPCR) in the AGD-resistant and AGD-susceptible animals, as well as non-infected nave fish. Gene expression determined by qPCR was in strong agreement with the microarray analysis. A large number of differentially expressed genes were involved in immune and cell cycle responses. Resistant individuals displayed significantly higher expression of genes involved in adaptive immunity and negative regulation of the cell cycle. In contrast, AGD-susceptible individuals showed higher expression of acute phase proteins and positive regulators of the cell cycle. Combined with the gill histopathology, our results suggest AGD resistance is acquired rather than innately present, and that this resistance is for the most part associated with the dysregulation of immune and cell cycle pathways. 2008 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Wynne, JW O'Sullivan, MG Stone, G Cook, MT Nowak, BF Lovell, DR Taylor, RS Elliott, NG |
author_facet |
Wynne, JW O'Sullivan, MG Stone, G Cook, MT Nowak, BF Lovell, DR Taylor, RS Elliott, NG |
author_sort |
Wynne, JW |
title |
Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways |
title_short |
Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways |
title_full |
Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways |
title_fullStr |
Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways |
title_full_unstemmed |
Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways |
title_sort |
resistance to amoebic gill disease (agd) is characterised by the transcriptional dysregulation of immune and cell cycle pathways |
publisher |
Elsevier Sci Ltd |
publishDate |
2008 |
url |
https://doi.org/10.1016/j.dci.2008.05.013 http://www.ncbi.nlm.nih.gov/pubmed/18621418 http://ecite.utas.edu.au/55463 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://dx.doi.org/10.1016/j.dci.2008.05.013 Wynne, JW and O'Sullivan, MG and Stone, G and Cook, MT and Nowak, BF and Lovell, DR and Taylor, RS and Elliott, NG, Resistance to amoebic gill disease (AGD) is characterised by the transcriptional dysregulation of immune and cell cycle pathways, Developmental and Comparative Immunology, 32, (12) pp. 1539 - 1560. ISSN 0145-305X (2008) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/18621418 http://ecite.utas.edu.au/55463 |
op_doi |
https://doi.org/10.1016/j.dci.2008.05.013 |
container_title |
Developmental & Comparative Immunology |
container_volume |
32 |
container_issue |
12 |
container_start_page |
1539 |
op_container_end_page |
1560 |
_version_ |
1766361306487586816 |