Identification and characterization of a novel intraepithelial lymphoid tissue in the gills of Atlantic salmon
In addition to being the respiratory organ in fish, the gills form a barrier against the external milieu. Innate and adaptive immune system components have been detected in the gills, but lymphoid cell accumulations similar to that seen in the mammalian mucosa have not been described. The present in...
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ftunivtasecite:oai:ecite.utas.edu.au:55455 2023-05-15T15:31:23+02:00 Identification and characterization of a novel intraepithelial lymphoid tissue in the gills of Atlantic salmon Haugarvoll, E Bjerkas, I Nowak, BF Hordvik, I Koppang, EO 2008 http://dx.doi.org/10.111/j.1469-7580.2008.00943.x http://www.ncbi.nlm.nih.gov/pubmed/19172734 http://ecite.utas.edu.au/55455 en eng Blackwell Publishing http://dx.doi.org/10.111/j.1469-7580.2008.00943.x Haugarvoll, E and Bjerkas, I and Nowak, BF and Hordvik, I and Koppang, EO, Identification and characterization of a novel intraepithelial lymphoid tissue in the gills of Atlantic salmon, Journal of Anatomy, 213, (2) pp. 202 - 209. ISSN 0021-8782 (2008) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/19172734 http://ecite.utas.edu.au/55455 Agricultural and Veterinary Sciences Fisheries Sciences Fish Physiology and Genetics Refereed Article PeerReviewed 2008 ftunivtasecite 2019-12-13T21:28:04Z In addition to being the respiratory organ in fish, the gills form a barrier against the external milieu. Innate and adaptive immune system components have been detected in the gills, but lymphoid cell accumulations similar to that seen in the mammalian mucosa have not been described. The present investigations revealed cell accumulations on the caudal edge of interbranchial septum at the base of the gill filaments in the Atlantic salmon. Cytokeratin immunohistochemical staining and identification of a basal membrane and desmosome cell junctions by electron microscopy showed that the cell accumulation was located intraepithelially. Major histocompatibility complex (MHC) class II+ cells were detected by immunohistochemistry, and laser capture micro-dissection and subsequent RT-PCR analysis revealed expression of T-cell receptor transcripts in the investigated tissue, suggesting the presence of T cells. The intraepithelial tissue reported here may be a suitable location for immune surveillance of gill infections, as well as a target site for new vaccine approaches and investigations of epithelial immunity. This is the first description of a lymphocyte cell aggregation within a teleostian gill epithelium network, illustrating a phylogenetically early form of leukocyte accumulations in a respiratory organ. Journal compilation 2008 Anatomical Society of Great Britain and Ireland. Article in Journal/Newspaper Atlantic salmon eCite UTAS (University of Tasmania) |
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Open Polar |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Fish Physiology and Genetics |
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Agricultural and Veterinary Sciences Fisheries Sciences Fish Physiology and Genetics Haugarvoll, E Bjerkas, I Nowak, BF Hordvik, I Koppang, EO Identification and characterization of a novel intraepithelial lymphoid tissue in the gills of Atlantic salmon |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Fish Physiology and Genetics |
description |
In addition to being the respiratory organ in fish, the gills form a barrier against the external milieu. Innate and adaptive immune system components have been detected in the gills, but lymphoid cell accumulations similar to that seen in the mammalian mucosa have not been described. The present investigations revealed cell accumulations on the caudal edge of interbranchial septum at the base of the gill filaments in the Atlantic salmon. Cytokeratin immunohistochemical staining and identification of a basal membrane and desmosome cell junctions by electron microscopy showed that the cell accumulation was located intraepithelially. Major histocompatibility complex (MHC) class II+ cells were detected by immunohistochemistry, and laser capture micro-dissection and subsequent RT-PCR analysis revealed expression of T-cell receptor transcripts in the investigated tissue, suggesting the presence of T cells. The intraepithelial tissue reported here may be a suitable location for immune surveillance of gill infections, as well as a target site for new vaccine approaches and investigations of epithelial immunity. This is the first description of a lymphocyte cell aggregation within a teleostian gill epithelium network, illustrating a phylogenetically early form of leukocyte accumulations in a respiratory organ. Journal compilation 2008 Anatomical Society of Great Britain and Ireland. |
format |
Article in Journal/Newspaper |
author |
Haugarvoll, E Bjerkas, I Nowak, BF Hordvik, I Koppang, EO |
author_facet |
Haugarvoll, E Bjerkas, I Nowak, BF Hordvik, I Koppang, EO |
author_sort |
Haugarvoll, E |
title |
Identification and characterization of a novel intraepithelial lymphoid tissue in the gills of Atlantic salmon |
title_short |
Identification and characterization of a novel intraepithelial lymphoid tissue in the gills of Atlantic salmon |
title_full |
Identification and characterization of a novel intraepithelial lymphoid tissue in the gills of Atlantic salmon |
title_fullStr |
Identification and characterization of a novel intraepithelial lymphoid tissue in the gills of Atlantic salmon |
title_full_unstemmed |
Identification and characterization of a novel intraepithelial lymphoid tissue in the gills of Atlantic salmon |
title_sort |
identification and characterization of a novel intraepithelial lymphoid tissue in the gills of atlantic salmon |
publisher |
Blackwell Publishing |
publishDate |
2008 |
url |
http://dx.doi.org/10.111/j.1469-7580.2008.00943.x http://www.ncbi.nlm.nih.gov/pubmed/19172734 http://ecite.utas.edu.au/55455 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://dx.doi.org/10.111/j.1469-7580.2008.00943.x Haugarvoll, E and Bjerkas, I and Nowak, BF and Hordvik, I and Koppang, EO, Identification and characterization of a novel intraepithelial lymphoid tissue in the gills of Atlantic salmon, Journal of Anatomy, 213, (2) pp. 202 - 209. ISSN 0021-8782 (2008) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/19172734 http://ecite.utas.edu.au/55455 |
_version_ |
1766361878445948928 |