Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar , with Amoebic Gill Disease
Gills from Atlantic salmon with experimentally induced amoebic gill disease (Neoparamoeba spp.) were examined with transmission electron microscopy to assess pathology and host-cell responses. Amoebae were found either on the surface epithelium or with pseudopodia extending deeply into invaginations...
Published in: | Veterinary Pathology |
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American College of Veterinary Pathologists
2007
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Online Access: | https://doi.org/10.1354/vp.44-5-663 http://www.ncbi.nlm.nih.gov/pubmed/17846238 http://ecite.utas.edu.au/49809 |
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ftunivtasecite:oai:ecite.utas.edu.au:49809 2023-05-15T15:31:18+02:00 Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar , with Amoebic Gill Disease Lovy, J Becker, J Speare, J Wadowski, DW Wright, GM Powell, MD 2007 https://doi.org/10.1354/vp.44-5-663 http://www.ncbi.nlm.nih.gov/pubmed/17846238 http://ecite.utas.edu.au/49809 en eng American College of Veterinary Pathologists http://dx.doi.org/10.1354/vp.44-5-663 Lovy, J and Becker, J and Speare, J and Wadowski, DW and Wright, GM and Powell, MD, Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar , with Amoebic Gill Disease, Veterinary Pathology, 44, (5) pp. 663-671. ISSN 0300-9858 (2007) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/17846238 http://ecite.utas.edu.au/49809 Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Refereed Article PeerReviewed 2007 ftunivtasecite https://doi.org/10.1354/vp.44-5-663 2019-12-13T21:24:15Z Gills from Atlantic salmon with experimentally induced amoebic gill disease (Neoparamoeba spp.) were examined with transmission electron microscopy to assess pathology and host-cell responses. Amoebae were found either on the surface epithelium or with pseudopodia extending deeply into invaginations of epithelial cells. The amoebae had various densities along the plasma membrane and contained electron-dense deposits within their cytoplasm. Surface epithelial cells sloughed from the gills and had features consistent with apoptosis, including rounded shape, loss of surface microridges, and hypercondensation of nuclear chromatin. Affected areas of gills had fusion of secondary lamellae with interlamellar spaces occupied by mitotic epithelial cells and eosinophils. Eosinophils contained abundant fusiform-shaped granules that measured approximately 1 m long and 360 nm wide. The granule consisted of an electron-dense matrix with a central inclusion that was less electron-dense, consisting of particulate and fibrillar material. In many instances, the central inclusion appeared empty and 90% of the eosinophils had morphology suggestive of piecemeal degranulation. Also observed within affected areas were a few neutrophils, mucous cells releasing mucus, and a small number of dendritic-like cells. Article in Journal/Newspaper Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) Veterinary Pathology 44 5 663 671 |
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eCite UTAS (University of Tasmania) |
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language |
English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
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Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Lovy, J Becker, J Speare, J Wadowski, DW Wright, GM Powell, MD Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar , with Amoebic Gill Disease |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
description |
Gills from Atlantic salmon with experimentally induced amoebic gill disease (Neoparamoeba spp.) were examined with transmission electron microscopy to assess pathology and host-cell responses. Amoebae were found either on the surface epithelium or with pseudopodia extending deeply into invaginations of epithelial cells. The amoebae had various densities along the plasma membrane and contained electron-dense deposits within their cytoplasm. Surface epithelial cells sloughed from the gills and had features consistent with apoptosis, including rounded shape, loss of surface microridges, and hypercondensation of nuclear chromatin. Affected areas of gills had fusion of secondary lamellae with interlamellar spaces occupied by mitotic epithelial cells and eosinophils. Eosinophils contained abundant fusiform-shaped granules that measured approximately 1 m long and 360 nm wide. The granule consisted of an electron-dense matrix with a central inclusion that was less electron-dense, consisting of particulate and fibrillar material. In many instances, the central inclusion appeared empty and 90% of the eosinophils had morphology suggestive of piecemeal degranulation. Also observed within affected areas were a few neutrophils, mucous cells releasing mucus, and a small number of dendritic-like cells. |
format |
Article in Journal/Newspaper |
author |
Lovy, J Becker, J Speare, J Wadowski, DW Wright, GM Powell, MD |
author_facet |
Lovy, J Becker, J Speare, J Wadowski, DW Wright, GM Powell, MD |
author_sort |
Lovy, J |
title |
Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar , with Amoebic Gill Disease |
title_short |
Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar , with Amoebic Gill Disease |
title_full |
Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar , with Amoebic Gill Disease |
title_fullStr |
Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar , with Amoebic Gill Disease |
title_full_unstemmed |
Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar , with Amoebic Gill Disease |
title_sort |
ultrastructural examination of the host cellular response in the gills of atlantic salmon, salmo salar , with amoebic gill disease |
publisher |
American College of Veterinary Pathologists |
publishDate |
2007 |
url |
https://doi.org/10.1354/vp.44-5-663 http://www.ncbi.nlm.nih.gov/pubmed/17846238 http://ecite.utas.edu.au/49809 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://dx.doi.org/10.1354/vp.44-5-663 Lovy, J and Becker, J and Speare, J and Wadowski, DW and Wright, GM and Powell, MD, Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar , with Amoebic Gill Disease, Veterinary Pathology, 44, (5) pp. 663-671. ISSN 0300-9858 (2007) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/17846238 http://ecite.utas.edu.au/49809 |
op_doi |
https://doi.org/10.1354/vp.44-5-663 |
container_title |
Veterinary Pathology |
container_volume |
44 |
container_issue |
5 |
container_start_page |
663 |
op_container_end_page |
671 |
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1766361790584717312 |