Phylogeny of Neoparamoeba strains isolated from marine fish and invertebrates as inferred from SSU rDNA sequences
We characterised 9 strains selected from primary isolates referable to Paramoeba/Neoparamoeba spp. Based on ultrastructural study, 5 strains isolated from fish (amoebic gill disease [AGD]-affected Atlantic salmon and dead southern bluefin tuna), 1 strain from netting of a floating sea cage and 3 str...
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ftunivtasecite:oai:ecite.utas.edu.au:45384 2023-05-15T15:32:46+02:00 Phylogeny of Neoparamoeba strains isolated from marine fish and invertebrates as inferred from SSU rDNA sequences Dykova, I Nowak, BF Peckova, H Fiala, I Crosbie, PBB Dvorakova, H 2007 https://doi.org/10.3354/dao074057 http://www.ncbi.nlm.nih.gov/pubmed/17425264 http://ecite.utas.edu.au/45384 en eng Inter-Research http://dx.doi.org/10.3354/dao074057 Dykova, I and Nowak, BF and Peckova, H and Fiala, I and Crosbie, PBB and Dvorakova, H, Phylogeny of Neoparamoeba strains isolated from marine fish and invertebrates as inferred from SSU rDNA sequences, Diseases of Aquatic Organisms, 74, (1) pp. 57-65. ISSN 0177-5103 (2007) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/17425264 http://ecite.utas.edu.au/45384 Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Refereed Article PeerReviewed 2007 ftunivtasecite https://doi.org/10.3354/dao074057 2019-12-13T21:21:14Z We characterised 9 strains selected from primary isolates referable to Paramoeba/Neoparamoeba spp. Based on ultrastructural study, 5 strains isolated from fish (amoebic gill disease [AGD]-affected Atlantic salmon and dead southern bluefin tuna), 1 strain from netting of a floating sea cage and 3 strains isolated from invertebrates (sea urchins and crab) were assigned to the genus Neoparamoeba Page, 1987. Phylogenetic analyses based on SSU rDNA sequences revealed affiliations of newly introduced and previously analysed Neoparamoeba strains. Three strains from the invertebrates and 2 out of 3 strains from gills of southern bluefin tunas were members of the N. branchiphila clade, while the remaining, fish-isolated strains, as well as the fish cage strain, clustered within the clade of N. pemaquidensis. These findings and previous reports point to the possibility that N. pemaquidensis and N. branchiphila can affect both fish and invertebrates. A new potential fish host, southern bluefin tuna, was included in the list of farmed fish endangered by N. branchiphila. The sequence of P. eilhardi (Culture Collection of Algae and Protozoa [CCAP] strain 1560/2) appeared in all analyses among sequences of strain representatives of Neoparamoeba species, in a position well supported by bootstrap value, Bremer index and Bayesian posterior probability. Our research shows that isolation of additional strains from invertebrates and further analyses of relations between molecular data and morphological characters of the genera Paramoeba and Neoparamoeba are required. This complexity needs to be considered when attempting to define molecular markers for identification of Paramoeba/Neoparamoeba species in tissues of fish and invertebrates. Inter-Research 2007. Article in Journal/Newspaper Atlantic salmon eCite UTAS (University of Tasmania) Diseases of Aquatic Organisms 74 57 65 |
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Open Polar |
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eCite UTAS (University of Tasmania) |
op_collection_id |
ftunivtasecite |
language |
English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
spellingShingle |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Dykova, I Nowak, BF Peckova, H Fiala, I Crosbie, PBB Dvorakova, H Phylogeny of Neoparamoeba strains isolated from marine fish and invertebrates as inferred from SSU rDNA sequences |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
description |
We characterised 9 strains selected from primary isolates referable to Paramoeba/Neoparamoeba spp. Based on ultrastructural study, 5 strains isolated from fish (amoebic gill disease [AGD]-affected Atlantic salmon and dead southern bluefin tuna), 1 strain from netting of a floating sea cage and 3 strains isolated from invertebrates (sea urchins and crab) were assigned to the genus Neoparamoeba Page, 1987. Phylogenetic analyses based on SSU rDNA sequences revealed affiliations of newly introduced and previously analysed Neoparamoeba strains. Three strains from the invertebrates and 2 out of 3 strains from gills of southern bluefin tunas were members of the N. branchiphila clade, while the remaining, fish-isolated strains, as well as the fish cage strain, clustered within the clade of N. pemaquidensis. These findings and previous reports point to the possibility that N. pemaquidensis and N. branchiphila can affect both fish and invertebrates. A new potential fish host, southern bluefin tuna, was included in the list of farmed fish endangered by N. branchiphila. The sequence of P. eilhardi (Culture Collection of Algae and Protozoa [CCAP] strain 1560/2) appeared in all analyses among sequences of strain representatives of Neoparamoeba species, in a position well supported by bootstrap value, Bremer index and Bayesian posterior probability. Our research shows that isolation of additional strains from invertebrates and further analyses of relations between molecular data and morphological characters of the genera Paramoeba and Neoparamoeba are required. This complexity needs to be considered when attempting to define molecular markers for identification of Paramoeba/Neoparamoeba species in tissues of fish and invertebrates. Inter-Research 2007. |
format |
Article in Journal/Newspaper |
author |
Dykova, I Nowak, BF Peckova, H Fiala, I Crosbie, PBB Dvorakova, H |
author_facet |
Dykova, I Nowak, BF Peckova, H Fiala, I Crosbie, PBB Dvorakova, H |
author_sort |
Dykova, I |
title |
Phylogeny of Neoparamoeba strains isolated from marine fish and invertebrates as inferred from SSU rDNA sequences |
title_short |
Phylogeny of Neoparamoeba strains isolated from marine fish and invertebrates as inferred from SSU rDNA sequences |
title_full |
Phylogeny of Neoparamoeba strains isolated from marine fish and invertebrates as inferred from SSU rDNA sequences |
title_fullStr |
Phylogeny of Neoparamoeba strains isolated from marine fish and invertebrates as inferred from SSU rDNA sequences |
title_full_unstemmed |
Phylogeny of Neoparamoeba strains isolated from marine fish and invertebrates as inferred from SSU rDNA sequences |
title_sort |
phylogeny of neoparamoeba strains isolated from marine fish and invertebrates as inferred from ssu rdna sequences |
publisher |
Inter-Research |
publishDate |
2007 |
url |
https://doi.org/10.3354/dao074057 http://www.ncbi.nlm.nih.gov/pubmed/17425264 http://ecite.utas.edu.au/45384 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://dx.doi.org/10.3354/dao074057 Dykova, I and Nowak, BF and Peckova, H and Fiala, I and Crosbie, PBB and Dvorakova, H, Phylogeny of Neoparamoeba strains isolated from marine fish and invertebrates as inferred from SSU rDNA sequences, Diseases of Aquatic Organisms, 74, (1) pp. 57-65. ISSN 0177-5103 (2007) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/17425264 http://ecite.utas.edu.au/45384 |
op_doi |
https://doi.org/10.3354/dao074057 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
74 |
container_start_page |
57 |
op_container_end_page |
65 |
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1766363251212288000 |